Staphylococcus phage vB_SauS-phiIPLA88

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Dubowvirus; Staphylococcus virus IPLA88

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B7T0C0|B7T0C0_9CAUD Gp30 OS=Staphylococcus phage vB_SauS-phiIPLA88 OX=2681608 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 10.36SFNVQITYY10 pKa = 9.73TGMIEE15 pKa = 3.91EE16 pKa = 4.54TIEE19 pKa = 4.21AEE21 pKa = 4.2SLEE24 pKa = 4.2EE25 pKa = 4.29AEE27 pKa = 5.02NEE29 pKa = 4.01ARR31 pKa = 11.84DD32 pKa = 3.74IAMMEE37 pKa = 4.13VPFDD41 pKa = 3.87CDD43 pKa = 2.9EE44 pKa = 4.33YY45 pKa = 10.98EE46 pKa = 4.3INVEE50 pKa = 4.07EE51 pKa = 4.55NEE53 pKa = 4.08

Molecular weight: 6.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B7T0B5|B7T0B5_9CAUD Gp25 OS=Staphylococcus phage vB_SauS-phiIPLA88 OX=2681608 PE=4 SV=1
MM1 pKa = 7.26GSVVIINNKK10 pKa = 8.88PYY12 pKa = 10.71KK13 pKa = 10.17FNNFEE18 pKa = 4.16RR19 pKa = 11.84EE20 pKa = 4.17LMSKK24 pKa = 10.29RR25 pKa = 11.84GINAGIVSKK34 pKa = 10.43RR35 pKa = 11.84VRR37 pKa = 11.84GCWEE41 pKa = 3.53FSEE44 pKa = 5.81ALDD47 pKa = 4.19APYY50 pKa = 11.24GMHH53 pKa = 6.2LKK55 pKa = 10.12EE56 pKa = 4.03YY57 pKa = 10.85RR58 pKa = 11.84EE59 pKa = 4.09MKK61 pKa = 10.5QMEE64 pKa = 4.66KK65 pKa = 10.56VKK67 pKa = 10.07QARR70 pKa = 11.84LEE72 pKa = 4.06RR73 pKa = 11.84EE74 pKa = 3.62IARR77 pKa = 11.84QRR79 pKa = 11.84RR80 pKa = 11.84KK81 pKa = 9.2EE82 pKa = 3.79AEE84 pKa = 3.84LRR86 pKa = 11.84RR87 pKa = 11.84KK88 pKa = 9.75KK89 pKa = 10.2PHH91 pKa = 6.56LFNVPQKK98 pKa = 10.53HH99 pKa = 6.08SRR101 pKa = 11.84DD102 pKa = 3.97PYY104 pKa = 10.08WFDD107 pKa = 3.01VTYY110 pKa = 11.33NQMFKK115 pKa = 10.23KK116 pKa = 9.86WSEE119 pKa = 3.76AA120 pKa = 3.32

Molecular weight: 14.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

12877

53

1155

214.6

24.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.848 ± 0.423

0.45 ± 0.087

6.345 ± 0.326

7.129 ± 0.522

4.326 ± 0.172

5.584 ± 0.257

1.561 ± 0.152

7.075 ± 0.35

9.07 ± 0.375

7.339 ± 0.323

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.64 ± 0.182

7.129 ± 0.324

2.827 ± 0.231

4.085 ± 0.188

4.054 ± 0.248

5.762 ± 0.235

6.71 ± 0.295

6.438 ± 0.266

1.258 ± 0.177

4.372 ± 0.343
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski