Lachnospiraceae bacterium 2_1_46FAA

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2056 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F3BD92|F3BD92_9FIRM Aldo_ket_red domain-containing protein OS=Lachnospiraceae bacterium 2_1_46FAA OX=742723 GN=HMPREF9477_01912 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.16KK3 pKa = 10.12YY4 pKa = 10.24VCEE7 pKa = 4.06PCGYY11 pKa = 10.75VYY13 pKa = 10.63DD14 pKa = 4.92PEE16 pKa = 6.71LGDD19 pKa = 4.01PDD21 pKa = 4.87GGIAPGTAFEE31 pKa = 5.97DD32 pKa = 4.28IPDD35 pKa = 3.79DD36 pKa = 4.06WVCPICGLGKK46 pKa = 10.3DD47 pKa = 3.93VFVEE51 pKa = 4.39EE52 pKa = 4.45

Molecular weight: 5.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F3BCC1|F3BCC1_9FIRM DNA gyrase subunit A OS=Lachnospiraceae bacterium 2_1_46FAA OX=742723 GN=gyrA PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 4.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2056

0

2056

667679

20

3699

324.7

36.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.663 ± 0.056

1.32 ± 0.021

5.278 ± 0.049

8.539 ± 0.075

4.058 ± 0.04

6.955 ± 0.051

1.659 ± 0.023

7.637 ± 0.065

8.277 ± 0.048

8.616 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.975 ± 0.029

4.63 ± 0.046

3.005 ± 0.026

3.2 ± 0.027

4.023 ± 0.045

5.45 ± 0.036

5.599 ± 0.056

7.14 ± 0.043

0.862 ± 0.022

4.115 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski