Bifidobacterium simiarum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2025 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2M9HE10|A0A2M9HE10_9BIFI Cell filamentation protein Fic OS=Bifidobacterium simiarum OX=2045441 GN=CSQ87_07335 PE=4 SV=1
MM1 pKa = 8.4DD2 pKa = 6.74DD3 pKa = 3.56DD4 pKa = 4.21TLFKK8 pKa = 10.44EE9 pKa = 4.78FCEE12 pKa = 4.39EE13 pKa = 4.31GEE15 pKa = 4.42SMSLGDD21 pKa = 5.38LLEE24 pKa = 4.72EE25 pKa = 4.3YY26 pKa = 11.38ANVFHH31 pKa = 7.36AAFFIMGEE39 pKa = 4.05DD40 pKa = 3.72GPYY43 pKa = 10.67VSDD46 pKa = 4.25KK47 pKa = 10.8EE48 pKa = 4.58LRR50 pKa = 11.84DD51 pKa = 3.49WLNWCVFYY59 pKa = 10.81GKK61 pKa = 9.59PRR63 pKa = 11.84DD64 pKa = 3.95EE65 pKa = 4.48YY66 pKa = 10.88PLTNQDD72 pKa = 2.88

Molecular weight: 8.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2M9HCH3|A0A2M9HCH3_9BIFI Beta sliding clamp OS=Bifidobacterium simiarum OX=2045441 GN=CSQ87_09995 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.79MKK15 pKa = 9.36HH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84ALINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.79GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2025

0

2025

751035

29

3159

370.9

40.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.965 ± 0.059

0.88 ± 0.016

6.72 ± 0.053

5.607 ± 0.055

3.423 ± 0.033

8.405 ± 0.053

2.181 ± 0.024

5.252 ± 0.043

3.646 ± 0.048

8.736 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.592 ± 0.026

3.156 ± 0.035

4.7 ± 0.035

3.09 ± 0.032

6.497 ± 0.067

6.099 ± 0.054

6.042 ± 0.05

7.885 ± 0.048

1.362 ± 0.023

2.762 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski