Marinicauda pacifica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Maricaulales; Maricaulaceae; Marinicauda

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2862 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S2H6Z3|A0A4S2H6Z3_9PROT DNA ligase OS=Marinicauda pacifica OX=1133559 GN=ligA PE=3 SV=1
TT1 pKa = 7.46IIATSATLGLAGTAAHH17 pKa = 6.93ANHH20 pKa = 6.53HH21 pKa = 5.9MSGDD25 pKa = 3.52EE26 pKa = 4.28HH27 pKa = 6.57GASSDD32 pKa = 3.25AAAMFDD38 pKa = 3.72SHH40 pKa = 6.92LQQAFQAIDD49 pKa = 3.32TDD51 pKa = 4.56GNGSISQQEE60 pKa = 3.96WGNWQADD67 pKa = 3.65DD68 pKa = 3.76GFYY71 pKa = 10.72AARR74 pKa = 11.84FDD76 pKa = 4.97EE77 pKa = 4.85FATDD81 pKa = 4.3DD82 pKa = 4.08ADD84 pKa = 3.98GVDD87 pKa = 2.85WDD89 pKa = 4.69EE90 pKa = 3.92YY91 pKa = 10.39HH92 pKa = 6.93GAVTAMYY99 pKa = 7.52DD100 pKa = 3.67TSSLDD105 pKa = 3.19QQ106 pKa = 4.13

Molecular weight: 11.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S2HD73|A0A4S2HD73_9PROT Long-chain fatty acid--CoA ligase OS=Marinicauda pacifica OX=1133559 GN=E5162_01590 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.48AGRR28 pKa = 11.84QVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.49GRR39 pKa = 11.84KK40 pKa = 8.72RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2862

0

2862

911846

33

1732

318.6

34.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.984 ± 0.066

0.761 ± 0.015

6.222 ± 0.034

6.747 ± 0.039

3.735 ± 0.032

8.797 ± 0.046

1.894 ± 0.022

4.767 ± 0.03

2.662 ± 0.044

9.947 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.304 ± 0.023

2.348 ± 0.027

4.993 ± 0.033

3.032 ± 0.027

7.459 ± 0.049

5.56 ± 0.034

5.142 ± 0.03

7.112 ± 0.036

1.327 ± 0.022

2.207 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski