African cassava mosaic virus (isolate West Kenyan 844) (ACMV) (Cassava latent virus (isolate West Kenyan 844))

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; African cassava mosaic virus

Average proteome isoelectric point is 8.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P14982|REP_CLVK Replication-associated protein OS=African cassava mosaic virus (isolate West Kenyan 844) OX=10818 GN=AC1 PE=3 SV=1
MM1 pKa = 7.47DD2 pKa = 3.97TSVPVISSDD11 pKa = 3.91YY12 pKa = 8.59IHH14 pKa = 7.09SARR17 pKa = 11.84TEE19 pKa = 3.99YY20 pKa = 11.33KK21 pKa = 9.37LTNDD25 pKa = 3.85EE26 pKa = 5.06SPITLQFPSTLEE38 pKa = 3.82RR39 pKa = 11.84TRR41 pKa = 11.84VRR43 pKa = 11.84IMGKK47 pKa = 9.13CMKK50 pKa = 9.42VDD52 pKa = 3.32HH53 pKa = 6.42VVIEE57 pKa = 4.18YY58 pKa = 10.56RR59 pKa = 11.84NQVPFNAQGSVIVTIRR75 pKa = 11.84DD76 pKa = 3.29TRR78 pKa = 11.84LSDD81 pKa = 3.52EE82 pKa = 4.56QQDD85 pKa = 3.6QAQFTFPIGCNVDD98 pKa = 2.92LHH100 pKa = 6.37YY101 pKa = 10.89FSASYY106 pKa = 9.98FSIDD110 pKa = 5.73DD111 pKa = 3.72NVPWQLLYY119 pKa = 10.6KK120 pKa = 10.56VEE122 pKa = 4.46DD123 pKa = 3.88SNVKK127 pKa = 10.56NGITFAQIKK136 pKa = 10.36AKK138 pKa = 10.61LKK140 pKa = 10.61LSAAKK145 pKa = 10.11HH146 pKa = 4.81STDD149 pKa = 3.87IKK151 pKa = 10.67FKK153 pKa = 10.86QPTIKK158 pKa = 10.14ILSKK162 pKa = 10.75DD163 pKa = 3.81YY164 pKa = 11.52GPDD167 pKa = 3.6CVDD170 pKa = 4.09FWSVGKK176 pKa = 9.26PKK178 pKa = 10.29PIRR181 pKa = 11.84RR182 pKa = 11.84LIQNEE187 pKa = 4.1PGTDD191 pKa = 3.36YY192 pKa = 10.14DD193 pKa = 3.64TGPKK197 pKa = 9.31YY198 pKa = 10.55RR199 pKa = 11.84PITVQPGEE207 pKa = 3.88TWATKK212 pKa = 8.57STIGRR217 pKa = 11.84YY218 pKa = 7.16TSMRR222 pKa = 11.84YY223 pKa = 7.26TRR225 pKa = 11.84PNPIDD230 pKa = 3.84IDD232 pKa = 4.1DD233 pKa = 4.53SSSKK237 pKa = 10.61QYY239 pKa = 10.31TSEE242 pKa = 4.31AEE244 pKa = 3.91FPLRR248 pKa = 11.84GLHH251 pKa = 5.61QLPEE255 pKa = 4.2ASLDD259 pKa = 3.78PGDD262 pKa = 5.63SISQTQSMSKK272 pKa = 10.41KK273 pKa = 9.69DD274 pKa = 3.24IEE276 pKa = 4.67SIIEE280 pKa = 3.86QTVNKK285 pKa = 9.95CLIAHH290 pKa = 7.22RR291 pKa = 11.84GSSHH295 pKa = 7.39KK296 pKa = 10.74DD297 pKa = 3.0LL298 pKa = 5.31

Molecular weight: 33.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P14975|AV2_CLVK Protein AV2 OS=African cassava mosaic virus (isolate West Kenyan 844) OX=10818 PE=3 SV=1
MM1 pKa = 8.05PFRR4 pKa = 11.84DD5 pKa = 4.21TYY7 pKa = 11.02IMSPINRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84CSRR19 pKa = 11.84VSLVEE24 pKa = 3.91CLQLTWSTXEE34 pKa = 4.15LLVFEE39 pKa = 5.45FKK41 pKa = 10.66PRR43 pKa = 11.84MSFSHH48 pKa = 5.46TQSVLYY54 pKa = 9.45PKK56 pKa = 9.15NTYY59 pKa = 9.51CHH61 pKa = 6.02SFKK64 pKa = 10.88HH65 pKa = 5.53FLSNQTLSSLKK76 pKa = 10.5SVEE79 pKa = 3.91SCIRR83 pKa = 11.84MGNLTCMPSFNSRR96 pKa = 11.84VKK98 pKa = 10.55SRR100 pKa = 11.84LRR102 pKa = 11.84TIVSSIVYY110 pKa = 7.12TQAVAPVSTPTFKK123 pKa = 11.01VPNQARR129 pKa = 11.84MSSPIWIRR137 pKa = 11.84TATPSNGDD145 pKa = 3.33NFKK148 pKa = 11.51SMDD151 pKa = 4.11DD152 pKa = 3.52LLEE155 pKa = 4.22AVNNQRR161 pKa = 11.84MMLTPKK167 pKa = 10.4RR168 pKa = 11.84LTAAVSQKK176 pKa = 11.4LLMSLGNN183 pKa = 3.61

Molecular weight: 20.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1735

113

358

216.9

24.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.611 ± 0.423

2.017 ± 0.207

4.784 ± 0.631

4.035 ± 0.412

4.496 ± 0.373

5.014 ± 0.483

3.804 ± 0.587

6.052 ± 0.794

5.764 ± 0.452

7.205 ± 0.534

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.017 ± 0.448

5.821 ± 0.591

5.937 ± 0.35

5.13 ± 0.398

6.744 ± 0.755

8.818 ± 1.299

5.937 ± 0.581

6.571 ± 0.57

1.21 ± 0.176

3.977 ± 0.505
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski