Cupriavidus sp. UYMMa02A

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Cupriavidus; unclassified Cupriavidus

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4000 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E4QNG5|A0A1E4QNG5_9BURK Gly-zipper_OmpA domain-containing protein OS=Cupriavidus sp. UYMMa02A OX=1774745 GN=AWV79_01930 PE=4 SV=1
MM1 pKa = 7.11AQCDD5 pKa = 3.85ATDD8 pKa = 3.98GVQDD12 pKa = 4.85GVIQTPGACNLDD24 pKa = 3.67MNKK27 pKa = 9.98LVASGVVTADD37 pKa = 3.23EE38 pKa = 4.44ASALSEE44 pKa = 4.2YY45 pKa = 10.42FSAVRR50 pKa = 11.84DD51 pKa = 3.41EE52 pKa = 4.91DD53 pKa = 4.23GNVIFPGSPLSAMNGPLPTPPLPTNVTGLDD83 pKa = 3.03SFMPINKK90 pKa = 9.64KK91 pKa = 10.42LVDD94 pKa = 3.96PNGFLPYY101 pKa = 10.25AGAIGDD107 pKa = 3.85QPINWVLLYY116 pKa = 10.79GYY118 pKa = 10.22VGLLAYY124 pKa = 10.7QNDD127 pKa = 4.43DD128 pKa = 3.71GGHH131 pKa = 5.45GG132 pKa = 3.35

Molecular weight: 13.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E4QS54|A0A1E4QS54_9BURK Transcription elongation factor GreA OS=Cupriavidus sp. UYMMa02A OX=1774745 GN=greA PE=3 SV=1
MM1 pKa = 7.75LSPPAARR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.97KK11 pKa = 10.61LLLRR15 pKa = 11.84PLTLLLRR22 pKa = 11.84LQPLPPLKK30 pKa = 10.62LRR32 pKa = 11.84LRR34 pKa = 11.84LLKK37 pKa = 10.04PRR39 pKa = 11.84PLRR42 pKa = 11.84LLKK45 pKa = 10.11PRR47 pKa = 11.84PRR49 pKa = 11.84LLKK52 pKa = 10.14PRR54 pKa = 11.84LRR56 pKa = 11.84PLTLRR61 pKa = 11.84PPAAKK66 pKa = 9.78QQ67 pKa = 3.07

Molecular weight: 7.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4000

0

4000

957226

29

1610

239.3

26.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.495 ± 0.061

1.11 ± 0.016

5.235 ± 0.03

5.264 ± 0.039

3.567 ± 0.028

8.226 ± 0.036

2.379 ± 0.02

4.618 ± 0.032

3.123 ± 0.038

10.205 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.682 ± 0.021

2.698 ± 0.025

5.15 ± 0.031

3.747 ± 0.025

7.234 ± 0.045

5.562 ± 0.032

5.22 ± 0.028

7.647 ± 0.036

1.417 ± 0.017

2.418 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski