Recombinant vesicular stomatitis Indiana virus rVSV-G/GFP

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Vesiculovirus; unclassified Vesiculovirus; recombinant Vesiculovirus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B7UCZ4|B7UCZ4_9RHAB Matrix protein OS=Recombinant vesicular stomatitis Indiana virus rVSV-G/GFP OX=582817 GN=M PE=3 SV=1
MM1 pKa = 8.26DD2 pKa = 5.12NLTKK6 pKa = 10.36VRR8 pKa = 11.84EE9 pKa = 4.07YY10 pKa = 10.74LKK12 pKa = 10.75SYY14 pKa = 10.66SRR16 pKa = 11.84LDD18 pKa = 3.47QAVGEE23 pKa = 4.08IDD25 pKa = 3.96EE26 pKa = 5.27IEE28 pKa = 3.97AQRR31 pKa = 11.84AEE33 pKa = 3.95KK34 pKa = 10.72SNYY37 pKa = 9.35EE38 pKa = 4.03LFQEE42 pKa = 4.94DD43 pKa = 3.8GVEE46 pKa = 4.08EE47 pKa = 4.23HH48 pKa = 6.6TKK50 pKa = 10.39PSYY53 pKa = 10.31FQAADD58 pKa = 3.66DD59 pKa = 4.51SDD61 pKa = 4.18TEE63 pKa = 4.31SEE65 pKa = 5.06PEE67 pKa = 3.81IEE69 pKa = 4.88DD70 pKa = 3.6NQGLYY75 pKa = 10.79APDD78 pKa = 4.29PEE80 pKa = 5.69AEE82 pKa = 4.11QVEE85 pKa = 4.84GFIQGPLDD93 pKa = 4.24DD94 pKa = 4.68YY95 pKa = 11.81ADD97 pKa = 3.85EE98 pKa = 4.35EE99 pKa = 4.7VDD101 pKa = 4.84VVFTSDD107 pKa = 2.64WKK109 pKa = 10.68QPEE112 pKa = 4.5LEE114 pKa = 4.1SDD116 pKa = 3.51EE117 pKa = 4.45HH118 pKa = 6.73GKK120 pKa = 7.3TLRR123 pKa = 11.84LTSPEE128 pKa = 4.05GLSGEE133 pKa = 4.78QKK135 pKa = 10.45SQWLSTIKK143 pKa = 10.75AVVQSAKK150 pKa = 9.7YY151 pKa = 8.96WNLAEE156 pKa = 4.46CTFEE160 pKa = 4.44ASGEE164 pKa = 4.14GVIMKK169 pKa = 9.27EE170 pKa = 3.92RR171 pKa = 11.84QITPDD176 pKa = 3.32VYY178 pKa = 11.16KK179 pKa = 9.69VTPVMNTHH187 pKa = 7.32PSQSEE192 pKa = 3.94AVSDD196 pKa = 3.56VWSLSKK202 pKa = 9.99TSMTFQPKK210 pKa = 9.52KK211 pKa = 10.78ASLQPLTISLDD222 pKa = 3.55EE223 pKa = 4.62LFSSRR228 pKa = 11.84GEE230 pKa = 4.17FISVGGDD237 pKa = 2.49GRR239 pKa = 11.84MSHH242 pKa = 6.7KK243 pKa = 10.31EE244 pKa = 3.81AILLGLRR251 pKa = 11.84YY252 pKa = 9.68KK253 pKa = 10.6KK254 pKa = 10.43LYY256 pKa = 8.53NQARR260 pKa = 11.84VKK262 pKa = 11.01YY263 pKa = 9.84SLL265 pKa = 3.74

Molecular weight: 29.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B7UCZ6|B7UCZ6_9RHAB G protein/GFP fusion protein OS=Recombinant vesicular stomatitis Indiana virus rVSV-G/GFP OX=582817 GN=G PE=3 SV=1
MM1 pKa = 7.66SSLKK5 pKa = 10.5KK6 pKa = 10.4ILGLKK11 pKa = 10.33GKK13 pKa = 9.61GKK15 pKa = 10.22KK16 pKa = 9.31SKK18 pKa = 10.43KK19 pKa = 10.13LGIAPPPYY27 pKa = 10.54EE28 pKa = 4.65EE29 pKa = 4.38DD30 pKa = 2.89TSMEE34 pKa = 4.29YY35 pKa = 10.77APSAPIDD42 pKa = 3.43KK43 pKa = 10.59SYY45 pKa = 11.26FGVDD49 pKa = 3.22EE50 pKa = 4.6MDD52 pKa = 3.53TYY54 pKa = 11.79DD55 pKa = 4.07PNQLRR60 pKa = 11.84YY61 pKa = 9.4EE62 pKa = 4.19KK63 pKa = 10.82FFFTVKK69 pKa = 7.98MTVRR73 pKa = 11.84SNRR76 pKa = 11.84PFRR79 pKa = 11.84TYY81 pKa = 11.11SDD83 pKa = 3.1VAAAVSHH90 pKa = 6.89WDD92 pKa = 3.04HH93 pKa = 6.65MYY95 pKa = 10.57IGMAGKK101 pKa = 10.33RR102 pKa = 11.84PFYY105 pKa = 10.56KK106 pKa = 9.97ILAFLGSSNLKK117 pKa = 8.58ATPAVLADD125 pKa = 3.47QGQPEE130 pKa = 4.26YY131 pKa = 10.24HH132 pKa = 6.14AHH134 pKa = 6.04CEE136 pKa = 3.81GRR138 pKa = 11.84AYY140 pKa = 10.7LPHH143 pKa = 7.54RR144 pKa = 11.84MGKK147 pKa = 7.15TPPMLNVPEE156 pKa = 4.26HH157 pKa = 5.81FRR159 pKa = 11.84RR160 pKa = 11.84PFNIGLYY167 pKa = 10.12KK168 pKa = 10.06GTIEE172 pKa = 4.18LTMTIYY178 pKa = 10.98DD179 pKa = 4.26DD180 pKa = 4.44EE181 pKa = 4.86SLEE184 pKa = 4.19AAPMIWDD191 pKa = 4.28HH192 pKa = 6.34FNSSKK197 pKa = 10.57FSDD200 pKa = 3.82FRR202 pKa = 11.84EE203 pKa = 3.96KK204 pKa = 11.25ALMFGLIVEE213 pKa = 5.14KK214 pKa = 10.15KK215 pKa = 10.74ASGAWVLDD223 pKa = 4.09SIGHH227 pKa = 5.98FKK229 pKa = 10.86

Molecular weight: 26.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

1

6

4286

229

2109

714.3

81.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.946 ± 0.477

1.703 ± 0.27

6.09 ± 0.331

5.576 ± 0.561

4.573 ± 0.2

6.696 ± 0.419

2.893 ± 0.26

6.556 ± 0.568

6.533 ± 0.32

9.263 ± 0.664

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.753 ± 0.198

4.06 ± 0.377

4.923 ± 0.183

3.383 ± 0.221

4.643 ± 0.743

8.283 ± 0.283

5.833 ± 0.142

5.413 ± 0.316

2.03 ± 0.158

3.85 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski