Hubei permutotetra-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KHP2|A0A1L3KHP2_9VIRU RdRp OS=Hubei permutotetra-like virus 1 OX=1923073 PE=4 SV=1
MM1 pKa = 7.98DD2 pKa = 4.25VPDD5 pKa = 4.59FNLDD9 pKa = 3.21KK10 pKa = 10.9STGDD14 pKa = 3.55LAIIAMSPTFSPFAKK29 pKa = 9.39NWTKK33 pKa = 10.19MRR35 pKa = 11.84VLDD38 pKa = 3.91AVSIKK43 pKa = 9.63WRR45 pKa = 11.84TNFVISQGGFTSLACAIKK63 pKa = 9.89PSKK66 pKa = 10.42DD67 pKa = 3.43YY68 pKa = 10.76TVMWTGQPLVDD79 pKa = 4.18TYY81 pKa = 10.29LTRR84 pKa = 11.84AGKK87 pKa = 10.58YY88 pKa = 7.05MVSALVDD95 pKa = 3.79VIFTCKK101 pKa = 9.59GAKK104 pKa = 9.59PLVFDD109 pKa = 6.0LEE111 pKa = 4.5WQLHH115 pKa = 5.89FGSHH119 pKa = 5.99WRR121 pKa = 11.84DD122 pKa = 2.59GWNFIIDD129 pKa = 3.74TAITAVSNSWFDD141 pKa = 3.78FSMTPLMPVRR151 pKa = 11.84KK152 pKa = 8.84PRR154 pKa = 11.84EE155 pKa = 3.63FPHH158 pKa = 6.73RR159 pKa = 11.84VRR161 pKa = 11.84KK162 pKa = 9.54RR163 pKa = 11.84DD164 pKa = 3.5LNAPFPPMTPPEE176 pKa = 4.22PAHH179 pKa = 4.87TQFYY183 pKa = 10.2FVDD186 pKa = 4.63DD187 pKa = 4.47YY188 pKa = 11.99YY189 pKa = 11.87SDD191 pKa = 4.51LGSTPP196 pKa = 3.25

Molecular weight: 22.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KHZ6|A0A1L3KHZ6_9VIRU Capsid protein OS=Hubei permutotetra-like virus 1 OX=1923073 PE=3 SV=1
MM1 pKa = 8.28DD2 pKa = 4.04KK3 pKa = 11.05RR4 pKa = 11.84KK5 pKa = 8.79MRR7 pKa = 11.84NQKK10 pKa = 9.75SRR12 pKa = 11.84SKK14 pKa = 10.85APQPQQRR21 pKa = 11.84RR22 pKa = 11.84KK23 pKa = 9.05QKK25 pKa = 9.77QKK27 pKa = 10.11PRR29 pKa = 11.84VVNITAGAPVTGKK42 pKa = 8.23EE43 pKa = 3.88TLLALKK49 pKa = 10.11VAANATTSSYY59 pKa = 11.04IKK61 pKa = 10.05TFTLVDD67 pKa = 3.99ASPILKK73 pKa = 9.97KK74 pKa = 10.76YY75 pKa = 8.31ATIYY79 pKa = 10.71DD80 pKa = 3.76SYY82 pKa = 10.64KK83 pKa = 9.77IKK85 pKa = 10.54SVAYY89 pKa = 10.07RR90 pKa = 11.84FVTDD94 pKa = 3.45EE95 pKa = 4.7ASTKK99 pKa = 10.39SGNVSLGIDD108 pKa = 3.47YY109 pKa = 8.96GKK111 pKa = 9.89PPSSLSRR118 pKa = 11.84DD119 pKa = 4.0SVCKK123 pKa = 9.48LTPHH127 pKa = 5.17YY128 pKa = 9.08TGPIKK133 pKa = 10.72KK134 pKa = 9.51NSPWVEE140 pKa = 3.52VSNRR144 pKa = 11.84FFNPNLVRR152 pKa = 11.84YY153 pKa = 10.18VSDD156 pKa = 3.34TDD158 pKa = 3.63TNTAPFSLCILLSCEE173 pKa = 3.74PTGAEE178 pKa = 3.84RR179 pKa = 11.84TLGALEE185 pKa = 4.08IKK187 pKa = 10.69YY188 pKa = 10.06SLEE191 pKa = 3.88MLGLQPP197 pKa = 5.32

Molecular weight: 21.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1438

196

1045

479.3

53.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.858 ± 0.461

0.695 ± 0.279

6.12 ± 0.576

5.702 ± 1.485

3.894 ± 0.893

5.424 ± 0.392

1.669 ± 0.287

4.242 ± 0.157

8.345 ± 0.665

8.554 ± 0.503

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.851 ± 0.36

3.964 ± 0.22

6.328 ± 0.75

3.408 ± 0.348

4.937 ± 0.158

6.537 ± 1.081

7.65 ± 0.355

6.954 ± 0.087

1.808 ± 0.439

3.06 ± 0.416
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski