Bean yellow dwarf virus (BeYDV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|O39522|REP_BEYDV Replication-associated protein OS=Bean yellow dwarf virus OX=57119 GN=C1/C2 PE=3 SV=2
MM1 pKa = 7.74PSASKK6 pKa = 10.72NFRR9 pKa = 11.84LQSKK13 pKa = 8.91YY14 pKa = 10.73VFLTYY19 pKa = 9.4PKK21 pKa = 10.21CSSQRR26 pKa = 11.84DD27 pKa = 3.76DD28 pKa = 4.51LFQFLWEE35 pKa = 4.41KK36 pKa = 9.4LTPFLIFFLGVASEE50 pKa = 3.94LHH52 pKa = 5.81QDD54 pKa = 3.44GTTHH58 pKa = 5.38YY59 pKa = 9.89HH60 pKa = 7.28ALIQLDD66 pKa = 4.16KK67 pKa = 10.33KK68 pKa = 10.7PCIRR72 pKa = 11.84DD73 pKa = 3.12PSFFDD78 pKa = 4.7FEE80 pKa = 4.9GNHH83 pKa = 6.71PNIQPARR90 pKa = 11.84NSKK93 pKa = 10.03QVLDD97 pKa = 4.6YY98 pKa = 10.62ISKK101 pKa = 11.02DD102 pKa = 3.01GDD104 pKa = 3.15IKK106 pKa = 10.39TRR108 pKa = 11.84GDD110 pKa = 3.45FRR112 pKa = 11.84DD113 pKa = 3.69HH114 pKa = 6.43KK115 pKa = 10.64VSPRR119 pKa = 11.84KK120 pKa = 9.71SDD122 pKa = 3.27ARR124 pKa = 11.84WRR126 pKa = 11.84TIIQTATSKK135 pKa = 11.17EE136 pKa = 4.18EE137 pKa = 3.74YY138 pKa = 10.43LDD140 pKa = 3.78MIKK143 pKa = 10.68EE144 pKa = 4.22EE145 pKa = 5.22FPHH148 pKa = 6.19EE149 pKa = 4.13WATKK153 pKa = 8.91LQWLEE158 pKa = 3.62YY159 pKa = 9.89SANKK163 pKa = 9.86LFPPQPEE170 pKa = 4.31QYY172 pKa = 10.21VSPFTEE178 pKa = 3.71SDD180 pKa = 3.68LRR182 pKa = 11.84CHH184 pKa = 6.95EE185 pKa = 5.28DD186 pKa = 3.03LHH188 pKa = 6.66NWRR191 pKa = 11.84EE192 pKa = 3.71THH194 pKa = 6.76LYY196 pKa = 9.34HH197 pKa = 8.28VSIDD201 pKa = 3.45AYY203 pKa = 9.35TFIHH207 pKa = 6.3PVSYY211 pKa = 10.78DD212 pKa = 3.32QAQSDD217 pKa = 4.79LEE219 pKa = 4.29WMADD223 pKa = 3.48LTRR226 pKa = 11.84MRR228 pKa = 11.84EE229 pKa = 4.18GLGSDD234 pKa = 3.77TPASTSADD242 pKa = 3.21QLVPEE247 pKa = 5.18RR248 pKa = 11.84PPGLEE253 pKa = 3.68VSGDD257 pKa = 3.57TTTGTGPSTSPTTMNTPPIISSTTSPSSSSHH288 pKa = 6.28CGSNN292 pKa = 3.08

Molecular weight: 33.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|O39521|REPA_BEYDV Replication-associated protein A OS=Bean yellow dwarf virus OX=57119 GN=C1 PE=1 SV=1
MM1 pKa = 7.24STVTWGKK8 pKa = 9.37KK9 pKa = 7.77RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84SGGDD16 pKa = 2.89SKK18 pKa = 11.57AKK20 pKa = 10.32SRR22 pKa = 11.84GSSSYY27 pKa = 9.95IPRR30 pKa = 11.84SVSSRR35 pKa = 11.84RR36 pKa = 11.84EE37 pKa = 3.91SLQVATFSWTSSGSGIKK54 pKa = 10.46FSPGGAAYY62 pKa = 10.05LVSNFPQGANDD73 pKa = 3.63NCRR76 pKa = 11.84HH77 pKa = 4.98TNKK80 pKa = 9.3TVLYY84 pKa = 10.59KK85 pKa = 10.37FMAKK89 pKa = 8.15NTIYY93 pKa = 10.59LDD95 pKa = 3.23SSHH98 pKa = 6.39YY99 pKa = 9.54MKK101 pKa = 10.76VFRR104 pKa = 11.84APFHH108 pKa = 5.97FWLVYY113 pKa = 10.42DD114 pKa = 4.69KK115 pKa = 11.5SPGANVPSTGDD126 pKa = 3.02IFEE129 pKa = 4.83GPSLFPHH136 pKa = 6.57NPWTWTVSRR145 pKa = 11.84AACHH149 pKa = 5.68RR150 pKa = 11.84FVVKK154 pKa = 9.21KK155 pKa = 8.11TWSCVVEE162 pKa = 4.47SNGIDD167 pKa = 3.43PTKK170 pKa = 10.86GQGATYY176 pKa = 9.79YY177 pKa = 11.18GPGPCNQVKK186 pKa = 10.24SCNKK190 pKa = 8.07FFKK193 pKa = 10.55RR194 pKa = 11.84LGVSTEE200 pKa = 3.88WKK202 pKa = 10.26NSATGDD208 pKa = 3.43VGDD211 pKa = 4.03IKK213 pKa = 10.92EE214 pKa = 4.06GALYY218 pKa = 10.17IVGAPSQKK226 pKa = 10.34SDD228 pKa = 3.54VYY230 pKa = 9.4VNGYY234 pKa = 8.88FRR236 pKa = 11.84VYY238 pKa = 10.14FKK240 pKa = 11.17SVGNQQ245 pKa = 2.8

Molecular weight: 27.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

963

92

334

240.8

27.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.569 ± 0.576

1.869 ± 0.262

6.438 ± 0.886

4.465 ± 0.86

5.815 ± 0.246

6.023 ± 1.161

3.427 ± 0.599

4.569 ± 0.566

6.854 ± 0.727

6.542 ± 0.968

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.454 ± 0.099

3.738 ± 0.537

6.542 ± 0.557

4.258 ± 0.556

4.777 ± 0.214

9.657 ± 1.172

7.373 ± 0.533

4.984 ± 1.404

2.285 ± 0.355

4.361 ± 0.306
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski