Nostoc phage A1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A191SAY2|A0A191SAY2_9CAUD LurX regulator protein OS=Nostoc phage A1 OX=1775256 PE=4 SV=1
MM1 pKa = 7.63SISDD5 pKa = 3.97IDD7 pKa = 3.56IEE9 pKa = 5.79IYY11 pKa = 10.85GEE13 pKa = 4.14WEE15 pKa = 3.66NDD17 pKa = 3.07TSKK20 pKa = 10.5FWNWCDD26 pKa = 2.93HH27 pKa = 7.04CDD29 pKa = 4.25RR30 pKa = 11.84ITTVICTVDD39 pKa = 3.39PYY41 pKa = 10.83IDD43 pKa = 3.93EE44 pKa = 5.01LYY46 pKa = 10.87KK47 pKa = 10.8DD48 pKa = 4.75DD49 pKa = 5.93EE50 pKa = 5.0IDD52 pKa = 3.43NTEE55 pKa = 4.36SNWCRR60 pKa = 11.84VCYY63 pKa = 9.99CKK65 pKa = 10.4RR66 pKa = 11.84KK67 pKa = 10.27EE68 pKa = 4.13EE69 pKa = 4.07II70 pKa = 3.73

Molecular weight: 8.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A191SB12|A0A191SB12_9CAUD Uncharacterized protein OS=Nostoc phage A1 OX=1775256 PE=4 SV=1
MM1 pKa = 7.62GKK3 pKa = 10.69GIGQSGYY10 pKa = 9.44RR11 pKa = 11.84GVRR14 pKa = 11.84KK15 pKa = 9.71HH16 pKa = 4.01YY17 pKa = 7.83TLWKK21 pKa = 10.29AVLPDD26 pKa = 3.54EE27 pKa = 4.64TVLGLYY33 pKa = 7.61ATPLDD38 pKa = 3.62AAIVRR43 pKa = 11.84EE44 pKa = 4.26RR45 pKa = 11.84FLHH48 pKa = 4.52EE49 pKa = 4.31HH50 pKa = 6.21RR51 pKa = 11.84DD52 pKa = 3.46EE53 pKa = 4.52FKK55 pKa = 10.9EE56 pKa = 3.89VEE58 pKa = 4.1RR59 pKa = 11.84YY60 pKa = 9.75LNHH63 pKa = 6.29VDD65 pKa = 4.15LAPGVIINTYY75 pKa = 10.2RR76 pKa = 11.84KK77 pKa = 8.19PKK79 pKa = 9.58KK80 pKa = 10.12VKK82 pKa = 8.43PQKK85 pKa = 9.5RR86 pKa = 11.84QPRR89 pKa = 11.84YY90 pKa = 8.87GKK92 pKa = 8.33YY93 pKa = 8.92VYY95 pKa = 10.39RR96 pKa = 11.84NRR98 pKa = 11.84HH99 pKa = 5.39HH100 pKa = 6.73NSFFVRR106 pKa = 11.84IGNYY110 pKa = 7.28QSKK113 pKa = 10.0CFPFVSYY120 pKa = 10.93AIAHH124 pKa = 6.38RR125 pKa = 11.84DD126 pKa = 3.12EE127 pKa = 4.37YY128 pKa = 11.11LQNNRR133 pKa = 11.84KK134 pKa = 9.14

Molecular weight: 15.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

19140

35

1021

199.4

22.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.899 ± 0.304

1.202 ± 0.117

5.841 ± 0.191

6.432 ± 0.347

4.718 ± 0.187

5.961 ± 0.362

1.594 ± 0.149

7.445 ± 0.265

7.356 ± 0.413

8.662 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.98 ± 0.123

6.118 ± 0.188

3.673 ± 0.152

3.809 ± 0.185

4.237 ± 0.242

6.708 ± 0.251

6.364 ± 0.247

6.609 ± 0.238

1.144 ± 0.08

4.248 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski