Sclerotium hydrophilum virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Curvulaviridae; Orthocurvulavirus; Sclerotium hydrophilum orthocurvulavirus 1

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B3SH34|A0A1B3SH34_9VIRU Putative RNA-dependent RNA polymerase OS=Sclerotium hydrophilum virus 1 OX=1895000 PE=4 SV=1
MM1 pKa = 7.14GWANGRR7 pKa = 11.84RR8 pKa = 11.84SPQDD12 pKa = 3.26VTWACFLYY20 pKa = 9.56YY21 pKa = 9.15WSRR24 pKa = 11.84MSAPPPLDD32 pKa = 3.86LLILEE37 pKa = 4.38EE38 pKa = 4.48HH39 pKa = 6.55AGEE42 pKa = 4.11PQVVISPEE50 pKa = 3.93APLVWRR56 pKa = 11.84MVAAWILRR64 pKa = 11.84VVGGDD69 pKa = 3.4VEE71 pKa = 4.18QLRR74 pKa = 11.84NLVGIVRR81 pKa = 11.84RR82 pKa = 11.84GDD84 pKa = 3.09MLTWIRR90 pKa = 11.84RR91 pKa = 11.84KK92 pKa = 10.24DD93 pKa = 3.23GRR95 pKa = 11.84AFFGNPSQPQAEE107 pKa = 4.25ALSRR111 pKa = 11.84LPEE114 pKa = 4.24PQWVEE119 pKa = 3.59ATEE122 pKa = 4.09EE123 pKa = 4.28EE124 pKa = 4.74EE125 pKa = 4.19GVEE128 pKa = 4.0MQEE131 pKa = 4.84GGTDD135 pKa = 3.04

Molecular weight: 15.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B3SH26|A0A1B3SH26_9VIRU Uncharacterized protein OS=Sclerotium hydrophilum virus 1 OX=1895000 PE=4 SV=1
MM1 pKa = 7.99ADD3 pKa = 3.49FQDD6 pKa = 3.17IPAARR11 pKa = 11.84RR12 pKa = 11.84AQGAGARR19 pKa = 11.84PTMAARR25 pKa = 11.84FGGAGGPVDD34 pKa = 3.83YY35 pKa = 10.24TDD37 pKa = 5.32RR38 pKa = 11.84PAHH41 pKa = 6.96DD42 pKa = 5.59DD43 pKa = 4.17EE44 pKa = 5.18ILPLPSPDD52 pKa = 4.04DD53 pKa = 3.72SFEE56 pKa = 4.17VLVDD60 pKa = 3.39KK61 pKa = 11.19LEE63 pKa = 4.31RR64 pKa = 11.84LAKK67 pKa = 9.91VVRR70 pKa = 11.84DD71 pKa = 3.93NKK73 pKa = 10.85SLALYY78 pKa = 9.19TGFHH82 pKa = 6.29ATPGGAINPVDD93 pKa = 3.47VAKK96 pKa = 10.7RR97 pKa = 11.84QFTTKK102 pKa = 9.91EE103 pKa = 3.62RR104 pKa = 11.84EE105 pKa = 4.08QYY107 pKa = 11.16AEE109 pKa = 3.96WEE111 pKa = 4.53AGSEE115 pKa = 4.34LPPFDD120 pKa = 3.86WNRR123 pKa = 11.84DD124 pKa = 3.59RR125 pKa = 11.84LPVAGKK131 pKa = 10.02DD132 pKa = 3.7GNPATLSKK140 pKa = 9.85WAEE143 pKa = 3.92ALSILWDD150 pKa = 3.54TKK152 pKa = 11.04GADD155 pKa = 3.34NGNVVWFKK163 pKa = 10.85KK164 pKa = 9.02SHH166 pKa = 7.01PYY168 pKa = 10.54LIPLVTAMVGVINARR183 pKa = 11.84KK184 pKa = 9.79GLTSGQAALSEE195 pKa = 4.35MEE197 pKa = 3.92MAEE200 pKa = 4.03YY201 pKa = 8.74QTARR205 pKa = 11.84KK206 pKa = 8.7IAAEE210 pKa = 3.88ATDD213 pKa = 4.02NIRR216 pKa = 11.84ITMEE220 pKa = 3.44AMRR223 pKa = 11.84RR224 pKa = 11.84PTEE227 pKa = 3.9RR228 pKa = 11.84MSAHH232 pKa = 5.69IQVIQEE238 pKa = 4.27RR239 pKa = 11.84SRR241 pKa = 11.84QLGNKK246 pKa = 9.35IPGKK250 pKa = 10.32DD251 pKa = 3.17RR252 pKa = 11.84GDD254 pKa = 3.44GPFNKK259 pKa = 9.43EE260 pKa = 3.5RR261 pKa = 11.84IALGKK266 pKa = 10.13RR267 pKa = 11.84RR268 pKa = 11.84VGVDD272 pKa = 3.6FEE274 pKa = 4.58VTHH277 pKa = 5.94NRR279 pKa = 11.84GAGRR283 pKa = 11.84EE284 pKa = 3.82VRR286 pKa = 11.84TRR288 pKa = 11.84HH289 pKa = 7.0DD290 pKa = 3.34IQQALAMPQRR300 pKa = 11.84PVASTSTTAPRR311 pKa = 11.84APPAPPTGSGSGTGAPGGFARR332 pKa = 11.84WGFGGSTGGFGSSLSQPAPPEE353 pKa = 4.23APPAGAQSPTYY364 pKa = 9.98RR365 pKa = 11.84PASPPPPSDD374 pKa = 3.37VPMAPGHH381 pKa = 5.91

Molecular weight: 40.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1140

135

624

380.0

42.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.298 ± 1.705

1.14 ± 0.595

5.965 ± 0.438

7.193 ± 1.117

4.123 ± 0.775

8.684 ± 0.778

2.018 ± 0.372

4.035 ± 0.216

4.737 ± 1.158

7.544 ± 1.099

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.07 ± 0.243

3.158 ± 0.31

6.93 ± 1.732

3.246 ± 0.603

7.719 ± 0.412

5.351 ± 0.279

4.912 ± 0.663

6.316 ± 0.759

1.754 ± 0.899

2.807 ± 0.771
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski