Cucumber mosaic virus (strain Q) (CMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Cucumovirus; Cucumber mosaic virus

Average proteome isoelectric point is 7.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q66125|2B_CMVQ Suppressor of silencing 2b OS=Cucumber mosaic virus (strain Q) OX=12310 GN=ORF2b PE=1 SV=1
MM1 pKa = 7.72ISPPPTFSFANLLNGSYY18 pKa = 10.84GVDD21 pKa = 3.02TPEE24 pKa = 3.53EE25 pKa = 4.2VEE27 pKa = 4.06RR28 pKa = 11.84VRR30 pKa = 11.84RR31 pKa = 11.84EE32 pKa = 3.74QRR34 pKa = 11.84EE35 pKa = 4.0DD36 pKa = 3.18AEE38 pKa = 4.31AALRR42 pKa = 11.84NYY44 pKa = 10.07KK45 pKa = 9.64PLPAVDD51 pKa = 3.8VSEE54 pKa = 4.26SVPRR58 pKa = 11.84DD59 pKa = 3.49EE60 pKa = 5.85PIVSQTVTAAPVTSVDD76 pKa = 3.5DD77 pKa = 4.2AFVSFGAEE85 pKa = 4.44DD86 pKa = 4.17YY87 pKa = 11.74LEE89 pKa = 4.32MSPSEE94 pKa = 3.96LLSAFEE100 pKa = 5.25LMVKK104 pKa = 9.47PLRR107 pKa = 11.84VGEE110 pKa = 4.19VLCSSFDD117 pKa = 3.29RR118 pKa = 11.84SLFISSVAMARR129 pKa = 11.84TLLLAPLTSTRR140 pKa = 11.84TLKK143 pKa = 10.69RR144 pKa = 11.84FEE146 pKa = 4.9DD147 pKa = 4.1LVAAIYY153 pKa = 10.48LKK155 pKa = 10.06TDD157 pKa = 4.17FFLEE161 pKa = 4.39DD162 pKa = 5.21DD163 pKa = 4.56GPQTDD168 pKa = 3.82VSQSDD173 pKa = 3.83VPGYY177 pKa = 10.1IFEE180 pKa = 5.44PGQHH184 pKa = 6.05SSGFEE189 pKa = 3.84PPPICAKK196 pKa = 10.32CDD198 pKa = 4.26LILYY202 pKa = 7.3QCPCFDD208 pKa = 4.27FNALRR213 pKa = 11.84EE214 pKa = 4.32SCAEE218 pKa = 3.78KK219 pKa = 10.15TFSHH223 pKa = 7.35DD224 pKa = 3.74YY225 pKa = 10.68VIEE228 pKa = 4.31GLDD231 pKa = 3.65GVIDD235 pKa = 3.91NATLLSNLGPFLLPVHH251 pKa = 6.63CSYY254 pKa = 11.74SKK256 pKa = 9.93TEE258 pKa = 4.45DD259 pKa = 3.45PDD261 pKa = 4.25FVVDD265 pKa = 4.49PSLARR270 pKa = 11.84PTDD273 pKa = 3.41RR274 pKa = 11.84VDD276 pKa = 3.14VHH278 pKa = 6.64VVQAVCDD285 pKa = 3.95TTLPTHH291 pKa = 6.64GNYY294 pKa = 10.09DD295 pKa = 3.56DD296 pKa = 5.53SFHH299 pKa = 6.76QVFVDD304 pKa = 3.45SADD307 pKa = 3.39YY308 pKa = 9.48STDD311 pKa = 3.1MDD313 pKa = 3.96HH314 pKa = 7.27VRR316 pKa = 11.84LRR318 pKa = 11.84QSDD321 pKa = 4.44LVAKK325 pKa = 10.26IPDD328 pKa = 4.31GGHH331 pKa = 6.04MLPVLNTGSGHH342 pKa = 5.29QRR344 pKa = 11.84VGTTKK349 pKa = 10.48EE350 pKa = 3.66VLTAIKK356 pKa = 9.97KK357 pKa = 10.04RR358 pKa = 11.84NADD361 pKa = 3.49VPEE364 pKa = 5.43LGDD367 pKa = 3.75SVNLSRR373 pKa = 11.84LSKK376 pKa = 10.62AVAEE380 pKa = 4.41RR381 pKa = 11.84FRR383 pKa = 11.84LSYY386 pKa = 10.7MNVDD390 pKa = 4.39ALAKK394 pKa = 10.03SNFVNVVSNFHH405 pKa = 7.84AYY407 pKa = 6.3MQKK410 pKa = 9.45WPSSGLSYY418 pKa = 11.44DD419 pKa = 4.67DD420 pKa = 5.94LPDD423 pKa = 3.54LHH425 pKa = 8.26AEE427 pKa = 3.82NLQFYY432 pKa = 10.96DD433 pKa = 4.19HH434 pKa = 6.9MIKK437 pKa = 10.37SDD439 pKa = 3.7VKK441 pKa = 10.7PVVTDD446 pKa = 3.18TLNVDD451 pKa = 3.69RR452 pKa = 11.84PVPATITFHH461 pKa = 7.02KK462 pKa = 9.73KK463 pKa = 8.81TITSQFSPLFISLFEE478 pKa = 4.05RR479 pKa = 11.84FQRR482 pKa = 11.84CLRR485 pKa = 11.84EE486 pKa = 4.05RR487 pKa = 11.84VVLPVGKK494 pKa = 9.96ISSLEE499 pKa = 3.79MTGFSVLNKK508 pKa = 9.86HH509 pKa = 6.15CLEE512 pKa = 4.71IDD514 pKa = 3.33LSKK517 pKa = 10.66FDD519 pKa = 3.64KK520 pKa = 11.1SQGEE524 pKa = 4.13FHH526 pKa = 7.92LMIQEE531 pKa = 5.03HH532 pKa = 6.55ILNDD536 pKa = 4.6LGCPAPITKK545 pKa = 8.63WWCDD549 pKa = 3.85FHH551 pKa = 8.08RR552 pKa = 11.84FSYY555 pKa = 10.65IKK557 pKa = 10.24DD558 pKa = 3.05KK559 pKa = 10.9RR560 pKa = 11.84AGVGMPISFQRR571 pKa = 11.84RR572 pKa = 11.84TGDD575 pKa = 2.78AFTYY579 pKa = 10.13FGNTIVTMAEE589 pKa = 4.01FAWCYY594 pKa = 10.05DD595 pKa = 2.91TDD597 pKa = 3.68QFDD600 pKa = 3.97RR601 pKa = 11.84LLFSGDD607 pKa = 3.51DD608 pKa = 3.31SLAFSKK614 pKa = 10.59LPPVGDD620 pKa = 3.47PSKK623 pKa = 10.39FTTLFNMEE631 pKa = 4.46AKK633 pKa = 10.42VMEE636 pKa = 4.39PAVPYY641 pKa = 9.94ICSKK645 pKa = 10.49FYY647 pKa = 11.44SLMSLVTRR655 pKa = 11.84FQSPTIRR662 pKa = 11.84EE663 pKa = 4.02IQRR666 pKa = 11.84LGTKK670 pKa = 10.02KK671 pKa = 10.19IPYY674 pKa = 9.41SDD676 pKa = 4.15NNDD679 pKa = 2.96FLFAHH684 pKa = 6.57FMSFVDD690 pKa = 3.62RR691 pKa = 11.84LKK693 pKa = 11.3FMDD696 pKa = 5.36RR697 pKa = 11.84MSQSCIDD704 pKa = 3.38QLSIFFEE711 pKa = 4.35LKK713 pKa = 9.87YY714 pKa = 10.59KK715 pKa = 10.81KK716 pKa = 10.27SGNEE720 pKa = 3.43AALVLGAFKK729 pKa = 10.8KK730 pKa = 9.51YY731 pKa = 7.93TANFNAYY738 pKa = 9.36KK739 pKa = 10.08EE740 pKa = 4.67LYY742 pKa = 10.2YY743 pKa = 10.53SDD745 pKa = 5.12RR746 pKa = 11.84QQCDD750 pKa = 3.15LVNTFCISEE759 pKa = 3.88FRR761 pKa = 11.84VIRR764 pKa = 11.84RR765 pKa = 11.84TTVKK769 pKa = 10.08KK770 pKa = 10.53KK771 pKa = 10.91KK772 pKa = 9.79NGCVDD777 pKa = 2.87SSGVDD782 pKa = 3.17RR783 pKa = 11.84RR784 pKa = 11.84PPLSQFAGGEE794 pKa = 4.1TSKK797 pKa = 10.06TKK799 pKa = 10.49VSRR802 pKa = 11.84QKK804 pKa = 10.15PASEE808 pKa = 4.46GLQKK812 pKa = 10.23SQRR815 pKa = 11.84EE816 pKa = 3.99SAIYY820 pKa = 10.56SEE822 pKa = 4.64TFPDD826 pKa = 3.42VTIPRR831 pKa = 11.84SRR833 pKa = 11.84SRR835 pKa = 11.84GLVSS839 pKa = 3.41

Molecular weight: 94.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q83267|Q83267_CMVQ Capsid protein OS=Cucumber mosaic virus (strain Q) OX=12310 PE=3 SV=1
MM1 pKa = 7.95DD2 pKa = 5.07KK3 pKa = 10.83SGSPNASRR11 pKa = 11.84TSGRR15 pKa = 11.84RR16 pKa = 11.84PRR18 pKa = 11.84RR19 pKa = 11.84GSRR22 pKa = 11.84SASGADD28 pKa = 2.93AGLRR32 pKa = 11.84ALTQQMLRR40 pKa = 11.84LNKK43 pKa = 8.8TLAIGRR49 pKa = 11.84PTLNHH54 pKa = 5.13QPSWVVKK61 pKa = 9.82AVNPGYY67 pKa = 8.78TFTSITLKK75 pKa = 10.54PPEE78 pKa = 4.38IEE80 pKa = 4.22KK81 pKa = 10.65GSCFGRR87 pKa = 11.84RR88 pKa = 11.84LSLPDD93 pKa = 3.75SVTDD97 pKa = 3.63YY98 pKa = 11.42DD99 pKa = 3.77KK100 pKa = 11.56KK101 pKa = 10.74LVSRR105 pKa = 11.84IQIRR109 pKa = 11.84INPLPKK115 pKa = 9.55FDD117 pKa = 3.58STVWVTVRR125 pKa = 11.84KK126 pKa = 9.96VPSSSDD132 pKa = 3.29LSVAAISAMFGDD144 pKa = 4.59GNSPVLVYY152 pKa = 9.68QYY154 pKa = 11.09AASGVQANNKK164 pKa = 9.16LLYY167 pKa = 10.29DD168 pKa = 4.29LSEE171 pKa = 3.92MRR173 pKa = 11.84ADD175 pKa = 3.14IGDD178 pKa = 3.55MRR180 pKa = 11.84KK181 pKa = 9.47YY182 pKa = 10.88AVLVYY187 pKa = 10.91SKK189 pKa = 10.91DD190 pKa = 3.64DD191 pKa = 3.61KK192 pKa = 11.49LEE194 pKa = 3.97KK195 pKa = 10.77DD196 pKa = 4.07EE197 pKa = 4.32IVFMSTSSINEE208 pKa = 4.08FLSHH212 pKa = 6.06GCSRR216 pKa = 11.84LSPCVYY222 pKa = 10.17RR223 pKa = 11.84RR224 pKa = 11.84PKK226 pKa = 8.57TLNYY230 pKa = 8.95TLNRR234 pKa = 11.84EE235 pKa = 4.37CC236 pKa = 5.61

Molecular weight: 26.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2663

100

991

443.8

49.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.834 ± 0.45

2.103 ± 0.355

6.647 ± 0.504

4.732 ± 0.463

4.957 ± 0.868

5.407 ± 0.367

2.253 ± 0.304

4.318 ± 0.349

5.745 ± 0.382

9.05 ± 0.167

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.591 ± 0.237

3.567 ± 0.448

4.957 ± 0.723

3.154 ± 0.236

6.234 ± 0.837

9.688 ± 0.825

5.971 ± 0.389

7.773 ± 0.276

0.901 ± 0.208

3.117 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski