Octopus bimaculoides (California two-spotted octopus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Spiralia; Lophotrochozoa; Mollusca; Cephalopoda; Coleoidea; Octopodiformes; Octopoda; Incirrata; Octopodidae; Octopus

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36239 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L8G9Y1|A0A0L8G9Y1_OCTBM Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22037958mg PE=4 SV=1
MM1 pKa = 7.73ADD3 pKa = 3.17EE4 pKa = 5.96GINNMTQPDD13 pKa = 4.13SVGNLLGDD21 pKa = 3.65SHH23 pKa = 8.04LGNLDD28 pKa = 3.72NYY30 pKa = 10.64SSSAFQSEE38 pKa = 4.32PLQPQPSAPHH48 pKa = 6.72QMMSSGSISSSNPLDD63 pKa = 4.12DD64 pKa = 5.91DD65 pKa = 4.58FGFDD69 pKa = 4.02SHH71 pKa = 6.28ITSSNTVNPSSNTTSSGSNNSAQSSSSYY99 pKa = 10.76TPGEE103 pKa = 4.5LISNTYY109 pKa = 10.64NPVDD113 pKa = 3.51HH114 pKa = 7.63DD115 pKa = 4.67SNYY118 pKa = 9.97NASRR122 pKa = 11.84VQIATHH128 pKa = 5.91DD129 pKa = 3.43TGEE132 pKa = 4.24NFNLDD137 pKa = 3.21NSNYY141 pKa = 9.91GMSDD145 pKa = 3.34SSPPPPLSQYY155 pKa = 7.42QQHH158 pKa = 6.61HH159 pKa = 6.54LNEE162 pKa = 4.54PSSKK166 pKa = 10.14VDD168 pKa = 3.49EE169 pKa = 4.49VDD171 pKa = 4.82SGDD174 pKa = 4.06SDD176 pKa = 4.07SDD178 pKa = 3.84DD179 pKa = 3.79YY180 pKa = 11.88EE181 pKa = 6.24KK182 pKa = 11.22YY183 pKa = 10.85DD184 pKa = 3.42HH185 pKa = 7.6DD186 pKa = 4.38SLVRR190 pKa = 11.84GQTVLTSKK198 pKa = 10.56HH199 pKa = 6.8SEE201 pKa = 4.2DD202 pKa = 3.64QQHH205 pKa = 6.06SQDD208 pKa = 4.22EE209 pKa = 4.43YY210 pKa = 11.66DD211 pKa = 5.67DD212 pKa = 4.84EE213 pKa = 7.61DD214 pKa = 6.16DD215 pKa = 5.24DD216 pKa = 5.65GEE218 pKa = 4.48SSLIEE223 pKa = 4.06YY224 pKa = 10.81NDD226 pKa = 3.7IEE228 pKa = 4.91DD229 pKa = 5.1DD230 pKa = 3.82EE231 pKa = 5.3VDD233 pKa = 3.79VVKK236 pKa = 11.18DD237 pKa = 3.34NDD239 pKa = 3.72FVYY242 pKa = 9.71STSAVADD249 pKa = 3.54SANAVDD255 pKa = 4.86KK256 pKa = 11.36FPDD259 pKa = 3.58NVQAPQLSPLDD270 pKa = 4.03SSLSPQTSPTHH281 pKa = 4.76QAPFHH286 pKa = 6.58SEE288 pKa = 3.48IPSFGSSEE296 pKa = 4.27ANDD299 pKa = 3.36VATISQSDD307 pKa = 3.51EE308 pKa = 4.15GKK310 pKa = 10.65VVDD313 pKa = 5.04SPSFMPSDD321 pKa = 3.61HH322 pKa = 7.15FITSTSDD329 pKa = 2.71AGTPSPSSVPVTSFGYY345 pKa = 10.42DD346 pKa = 3.32DD347 pKa = 4.36PLKK350 pKa = 10.76LSGVASSVEE359 pKa = 3.98TAAPSGQFMNDD370 pKa = 3.36LAPEE374 pKa = 4.31VIGGNDD380 pKa = 3.5GVEE383 pKa = 4.3VAGSEE388 pKa = 4.24NDD390 pKa = 3.96DD391 pKa = 4.02ADD393 pKa = 3.76SSDD396 pKa = 3.6EE397 pKa = 3.96QRR399 pKa = 11.84CEE401 pKa = 3.95IPAHH405 pKa = 6.48SSLSMSTPVVASANPSFSQPKK426 pKa = 8.28STSPPISDD434 pKa = 3.72PQLSVLNSDD443 pKa = 4.93PYY445 pKa = 10.49CTTIKK450 pKa = 10.08HH451 pKa = 4.98QQPSTFYY458 pKa = 8.3NTQSINEE465 pKa = 4.04NRR467 pKa = 11.84FSSEE471 pKa = 3.95PDD473 pKa = 3.11SSSEE477 pKa = 3.9PTVQTGPNFTEE488 pKa = 4.66PKK490 pKa = 10.16

Molecular weight: 52.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L8GMG5|A0A0L8GMG5_OCTBM Chitin-binding type-4 domain-containing protein (Fragment) OS=Octopus bimaculoides OX=37653 GN=OCBIM_22031095mg PE=4 SV=1
EEE2 pKa = 4.34EE3 pKa = 3.99KK4 pKa = 9.69AKKK7 pKa = 8.33RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84WRR27 pKa = 11.84RR28 pKa = 11.84WRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KKK38 pKa = 7.93RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84WRR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84RR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84RR77 pKa = 11.84KKK79 pKa = 9.13EE80 pKa = 3.49SMKKK84 pKa = 10.95NDDD87 pKa = 2.92VAISKKK93 pKa = 8.68K

Molecular weight: 13.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

32794

3445

36239

12291891

8

11437

339.2

38.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.069 ± 0.014

2.44 ± 0.016

5.42 ± 0.018

6.562 ± 0.027

3.911 ± 0.014

4.945 ± 0.017

3.066 ± 0.015

5.969 ± 0.018

7.152 ± 0.021

8.581 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.274 ± 0.007

5.44 ± 0.014

4.568 ± 0.016

4.309 ± 0.019

4.905 ± 0.016

9.067 ± 0.029

6.421 ± 0.026

5.803 ± 0.013

0.934 ± 0.005

3.154 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski