Phomopsis longicolla circular virus 1

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5QNP2|A0A1S5QNP2_9VIRU Putative coat protein OS=Phomopsis longicolla circular virus 1 OX=1808960 PE=4 SV=1
MM1 pKa = 8.2DD2 pKa = 4.6LTQLFLTSSPPPSHH16 pKa = 6.29QPNPLTLNIRR26 pKa = 11.84DD27 pKa = 4.1LSQDD31 pKa = 4.45DD32 pKa = 4.47FPQDD36 pKa = 3.69DD37 pKa = 3.91LSSFVVPDD45 pKa = 3.46SQFPGPEE52 pKa = 4.0ALPLSSPQFMTPLLHH67 pKa = 6.23TEE69 pKa = 4.3SLFVSQPDD77 pKa = 3.69EE78 pKa = 4.58DD79 pKa = 4.76DD80 pKa = 3.76QPEE83 pKa = 4.18DD84 pKa = 3.3QQDD87 pKa = 3.46AVGSDD92 pKa = 3.95DD93 pKa = 4.12EE94 pKa = 5.57LGLVDD99 pKa = 5.66PLDD102 pKa = 3.65EE103 pKa = 4.33TAGKK107 pKa = 9.76FRR109 pKa = 11.84LAAKK113 pKa = 10.05SVFLTYY119 pKa = 9.55PKK121 pKa = 10.34CHH123 pKa = 7.1LDD125 pKa = 3.17KK126 pKa = 11.06EE127 pKa = 4.82VFVKK131 pKa = 10.81AIEE134 pKa = 4.15KK135 pKa = 10.49FKK137 pKa = 10.86FDD139 pKa = 2.66QFYY142 pKa = 10.99AVRR145 pKa = 11.84EE146 pKa = 4.05DD147 pKa = 4.1HH148 pKa = 7.58KK149 pKa = 11.24DD150 pKa = 3.43GTPHH154 pKa = 5.17YY155 pKa = 9.51HH156 pKa = 7.2VIGEE160 pKa = 4.05WSVKK164 pKa = 10.54KK165 pKa = 10.4NIKK168 pKa = 9.77NPRR171 pKa = 11.84HH172 pKa = 5.88FDD174 pKa = 3.02IQKK177 pKa = 7.5YY178 pKa = 9.06HH179 pKa = 6.61PNIGRR184 pKa = 11.84TRR186 pKa = 11.84NRR188 pKa = 11.84LAAWRR193 pKa = 11.84YY194 pKa = 5.22VHH196 pKa = 5.81KK197 pKa = 9.66TKK199 pKa = 10.48GRR201 pKa = 11.84STHH204 pKa = 6.17VGGDD208 pKa = 3.22MPEE211 pKa = 3.8PVGIRR216 pKa = 11.84KK217 pKa = 7.79EE218 pKa = 4.13AKK220 pKa = 9.58DD221 pKa = 4.39DD222 pKa = 3.68FWKK225 pKa = 10.76EE226 pKa = 3.35AVTIKK231 pKa = 10.7DD232 pKa = 3.19RR233 pKa = 11.84NTFISRR239 pKa = 11.84FRR241 pKa = 11.84QEE243 pKa = 3.84APRR246 pKa = 11.84DD247 pKa = 4.0LIVHH251 pKa = 5.88YY252 pKa = 10.99SNVKK256 pKa = 10.3AYY258 pKa = 10.38ADD260 pKa = 3.92DD261 pKa = 3.92EE262 pKa = 5.08FKK264 pKa = 11.35VEE266 pKa = 3.91APEE269 pKa = 4.05YY270 pKa = 7.35CTPEE274 pKa = 3.62MSGYY278 pKa = 9.54WDD280 pKa = 3.84LPEE283 pKa = 4.38EE284 pKa = 3.98LKK286 pKa = 10.92EE287 pKa = 4.03WVEE290 pKa = 4.1EE291 pKa = 4.06NLTHH295 pKa = 7.66KK296 pKa = 10.29SARR299 pKa = 11.84PKK301 pKa = 10.48SLCLYY306 pKa = 10.94GEE308 pKa = 3.98TRR310 pKa = 11.84LYY312 pKa = 8.41KK313 pKa = 9.74TIWARR318 pKa = 11.84SLGPHH323 pKa = 6.62SYY325 pKa = 10.24MSGCWNARR333 pKa = 11.84LLDD336 pKa = 4.15DD337 pKa = 4.63DD338 pKa = 4.07KK339 pKa = 11.58QYY341 pKa = 11.79VVIDD345 pKa = 4.62DD346 pKa = 4.33VPLDD350 pKa = 4.1DD351 pKa = 5.99KK352 pKa = 12.17DD353 pKa = 3.64MFKK356 pKa = 10.63HH357 pKa = 5.86FKK359 pKa = 10.54QMLGCQNNFSVTDD372 pKa = 3.71KK373 pKa = 10.89YY374 pKa = 11.41VKK376 pKa = 10.13KK377 pKa = 10.59LHH379 pKa = 5.41FAKK382 pKa = 10.12WGLPCIYY389 pKa = 10.0LANQDD394 pKa = 3.36PRR396 pKa = 11.84EE397 pKa = 3.94YY398 pKa = 9.96STIDD402 pKa = 3.06GTHH405 pKa = 5.89RR406 pKa = 11.84RR407 pKa = 11.84WLNGNCVFVEE417 pKa = 4.17VEE419 pKa = 4.07KK420 pKa = 10.7PLFVASSGPGTPDD433 pKa = 2.49SRR435 pKa = 11.84QEE437 pKa = 3.9HH438 pKa = 6.8LSQFLQEE445 pKa = 4.4AEE447 pKa = 3.93QDD449 pKa = 3.44EE450 pKa = 5.0SFF452 pKa = 4.09

Molecular weight: 52.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5QNP2|A0A1S5QNP2_9VIRU Putative coat protein OS=Phomopsis longicolla circular virus 1 OX=1808960 PE=4 SV=1
MM1 pKa = 7.19VFGRR5 pKa = 11.84RR6 pKa = 11.84GGYY9 pKa = 8.94RR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 6.63FYY14 pKa = 10.81NSRR17 pKa = 11.84PYY19 pKa = 10.89YY20 pKa = 10.29RR21 pKa = 11.84FASAVNSVGQEE32 pKa = 3.99KK33 pKa = 10.62KK34 pKa = 10.74NKK36 pKa = 8.04TVSLAGPGSASAHH49 pKa = 6.08CEE51 pKa = 3.77MHH53 pKa = 7.14AGDD56 pKa = 4.7PDD58 pKa = 3.9SGFVLPATEE67 pKa = 4.26VCRR70 pKa = 11.84SGDD73 pKa = 3.22RR74 pKa = 11.84SSGRR78 pKa = 11.84EE79 pKa = 3.58VHH81 pKa = 6.77LRR83 pKa = 11.84GAKK86 pKa = 9.95LSFEE90 pKa = 4.14ICVSRR95 pKa = 11.84TCSVRR100 pKa = 11.84VLSVVLDD107 pKa = 3.65TDD109 pKa = 4.34RR110 pKa = 11.84NWFNVTGDD118 pKa = 3.62SVGLKK123 pKa = 8.36NTQNGGFGPLSGTGHH138 pKa = 7.01GIVHH142 pKa = 6.86FLDD145 pKa = 3.62VNNGVFSSEE154 pKa = 4.47DD155 pKa = 3.14LRR157 pKa = 11.84PWFFRR162 pKa = 11.84SVSKK166 pKa = 9.7RR167 pKa = 11.84TKK169 pKa = 10.63GRR171 pKa = 11.84ILLDD175 pKa = 3.35QVQHH179 pKa = 5.23FTVGDD184 pKa = 3.91RR185 pKa = 11.84FQFRR189 pKa = 11.84PVSAWLDD196 pKa = 3.28LHH198 pKa = 7.04GKK200 pKa = 7.7VTYY203 pKa = 10.08QQTSDD208 pKa = 2.86IVDD211 pKa = 3.37RR212 pKa = 11.84DD213 pKa = 4.31GVCTRR218 pKa = 11.84GIDD221 pKa = 3.43EE222 pKa = 4.72KK223 pKa = 10.77PYY225 pKa = 9.39NVYY228 pKa = 10.6FIVLVHH234 pKa = 6.09VPSFSTEE241 pKa = 3.7QVVRR245 pKa = 11.84QSIGMPDD252 pKa = 3.9VGRR255 pKa = 11.84MDD257 pKa = 3.56VDD259 pKa = 3.78DD260 pKa = 4.34PRR262 pKa = 11.84VPKK265 pKa = 10.33RR266 pKa = 11.84EE267 pKa = 3.79RR268 pKa = 11.84GIPGYY273 pKa = 10.07GSRR276 pKa = 11.84PSTAEE281 pKa = 3.88SEE283 pKa = 4.28ASTIILDD290 pKa = 3.88RR291 pKa = 11.84KK292 pKa = 9.64GVAVPAIEE300 pKa = 3.94MRR302 pKa = 11.84NISSALYY309 pKa = 9.03WRR311 pKa = 11.84EE312 pKa = 3.46

Molecular weight: 34.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

764

312

452

382.0

43.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.712 ± 0.132

1.571 ± 0.019

8.115 ± 0.999

5.759 ± 0.933

5.366 ± 0.14

6.806 ± 1.833

3.403 ± 0.304

3.403 ± 0.26

5.759 ± 1.308

7.199 ± 1.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.571 ± 0.019

3.403 ± 0.072

6.283 ± 0.864

3.927 ± 0.61

6.806 ± 1.645

7.984 ± 1.143

4.712 ± 0.056

8.246 ± 1.741

1.702 ± 0.246

3.272 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski