Halomonas sp. JB37

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas; unclassified Halomonas

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3369 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A3XBN5|A0A2A3XBN5_9GAMM Phenylacetic acid degradation protein OS=Halomonas sp. JB37 OX=2024405 GN=CIK78_02950 PE=4 SV=1
MM1 pKa = 7.76KK2 pKa = 9.33LTLSLTAATLLAASTAYY19 pKa = 9.74AQSYY23 pKa = 8.83PSEE26 pKa = 4.31PVTLVVPYY34 pKa = 10.47GPGGASDD41 pKa = 3.63LAGRR45 pKa = 11.84AFAEE49 pKa = 4.43SARR52 pKa = 11.84EE53 pKa = 3.9YY54 pKa = 10.41LGEE57 pKa = 5.04PITVVNQAGAGGMTGARR74 pKa = 11.84DD75 pKa = 3.66VTRR78 pKa = 11.84ADD80 pKa = 3.47PDD82 pKa = 4.05GYY84 pKa = 9.32TLLLARR90 pKa = 11.84VGMALSPAVNDD101 pKa = 3.64NSSVDD106 pKa = 2.92WDD108 pKa = 4.0EE109 pKa = 4.06YY110 pKa = 10.9TFISPLEE117 pKa = 4.22ATPMILAVADD127 pKa = 4.08DD128 pKa = 4.13SPYY131 pKa = 11.09EE132 pKa = 4.14SVEE135 pKa = 3.79ALLEE139 pKa = 4.72GIEE142 pKa = 4.12NDD144 pKa = 3.87PGAMSYY150 pKa = 10.49AASGATAIDD159 pKa = 4.15GFTVQALLADD169 pKa = 4.16AEE171 pKa = 4.37MDD173 pKa = 4.03PLTAATLIPYY183 pKa = 10.21RR184 pKa = 11.84GGGEE188 pKa = 4.09LATALLGGHH197 pKa = 6.08VDD199 pKa = 4.47FLAIAAASLMPHH211 pKa = 6.57IEE213 pKa = 4.21SGDD216 pKa = 3.59MRR218 pKa = 11.84PLMVFSPEE226 pKa = 3.91RR227 pKa = 11.84MATLPDD233 pKa = 3.43VPTAEE238 pKa = 4.01EE239 pKa = 4.23LGYY242 pKa = 10.54EE243 pKa = 4.36LAGQVIGWSALYY255 pKa = 10.18GPPDD259 pKa = 3.59LPEE262 pKa = 4.09EE263 pKa = 4.9VVTTWANVVEE273 pKa = 4.92QVAEE277 pKa = 4.17DD278 pKa = 4.19EE279 pKa = 4.62LWLSRR284 pKa = 11.84TEE286 pKa = 4.05DD287 pKa = 3.21RR288 pKa = 11.84GSISTIGSIDD298 pKa = 3.39MEE300 pKa = 4.76TYY302 pKa = 10.76AKK304 pKa = 9.87EE305 pKa = 3.66QYY307 pKa = 10.15EE308 pKa = 4.79FYY310 pKa = 10.72RR311 pKa = 11.84SLAEE315 pKa = 3.6QFGYY319 pKa = 11.1LEE321 pKa = 4.2

Molecular weight: 33.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A3XA83|A0A2A3XA83_9GAMM Uncharacterized protein OS=Halomonas sp. JB37 OX=2024405 GN=CIK78_17665 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.04RR14 pKa = 11.84AHH16 pKa = 6.12GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3369

0

3369

1110597

37

3262

329.7

36.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.878 ± 0.038

0.948 ± 0.014

5.463 ± 0.041

6.006 ± 0.042

3.626 ± 0.027

7.837 ± 0.035

2.43 ± 0.025

5.301 ± 0.04

3.044 ± 0.033

11.325 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.663 ± 0.021

2.998 ± 0.025

4.741 ± 0.025

4.277 ± 0.035

6.2 ± 0.041

6.001 ± 0.032

5.179 ± 0.025

7.147 ± 0.037

1.462 ± 0.018

2.475 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski