Tomato leaf curl Anjouan virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7DXH8|A7DXH8_9GEMI C4 protein OS=Tomato leaf curl Anjouan virus OX=439422 GN=c4 PE=3 SV=1
MM1 pKa = 7.34PLEE4 pKa = 3.93YY5 pKa = 9.82TLYY8 pKa = 10.13IVPLYY13 pKa = 10.54TDD15 pKa = 3.54ILVSCVYY22 pKa = 10.16PDD24 pKa = 3.92SKK26 pKa = 10.91RR27 pKa = 11.84QLKK30 pKa = 8.83MPRR33 pKa = 11.84AGRR36 pKa = 11.84FKK38 pKa = 10.78INAKK42 pKa = 10.25NYY44 pKa = 9.22FITYY48 pKa = 6.44PQCSITKK55 pKa = 9.75EE56 pKa = 3.63EE57 pKa = 4.24ALAQIKK63 pKa = 10.39SFSYY67 pKa = 7.82PTNIKK72 pKa = 10.01FIRR75 pKa = 11.84VCRR78 pKa = 11.84EE79 pKa = 3.4LHH81 pKa = 6.27QDD83 pKa = 3.85GLPHH87 pKa = 6.4LHH89 pKa = 6.55VLIQFEE95 pKa = 5.32GKK97 pKa = 9.21FQCTNQRR104 pKa = 11.84FFDD107 pKa = 4.08LVSQTRR113 pKa = 11.84SAHH116 pKa = 4.86FHH118 pKa = 6.57PNIQGAKK125 pKa = 9.45SSSDD129 pKa = 2.98VKK131 pKa = 11.11AYY133 pKa = 9.72IEE135 pKa = 4.28KK136 pKa = 10.72GGEE139 pKa = 3.8FLDD142 pKa = 3.8YY143 pKa = 11.32GVFQVDD149 pKa = 2.95ARR151 pKa = 11.84SARR154 pKa = 11.84GEE156 pKa = 4.23GQHH159 pKa = 6.45LAQVYY164 pKa = 10.81ADD166 pKa = 4.28ALNASSKK173 pKa = 11.05EE174 pKa = 3.77EE175 pKa = 3.55ALQIIKK181 pKa = 10.59EE182 pKa = 4.08KK183 pKa = 10.65DD184 pKa = 3.17PKK186 pKa = 11.22SFFLQYY192 pKa = 11.05HH193 pKa = 5.66NLSANADD200 pKa = 4.29KK201 pKa = 11.14IFMTPPNPYY210 pKa = 9.47VSNFLTSSFDD220 pKa = 3.47QVPEE224 pKa = 3.83EE225 pKa = 4.03LEE227 pKa = 3.64IWVSEE232 pKa = 4.08NVMDD236 pKa = 5.26AAARR240 pKa = 11.84PWRR243 pKa = 11.84PMSIVLEE250 pKa = 4.41GDD252 pKa = 3.1SRR254 pKa = 11.84TGKK257 pKa = 8.52TMWARR262 pKa = 11.84SLGPHH267 pKa = 6.71NYY269 pKa = 10.18LCGHH273 pKa = 7.38LDD275 pKa = 4.18LSPKK279 pKa = 10.15VYY281 pKa = 11.07SNDD284 pKa = 2.25AWYY287 pKa = 10.92NVIDD291 pKa = 5.2DD292 pKa = 4.41VDD294 pKa = 3.61PHH296 pKa = 5.85YY297 pKa = 11.0LKK299 pKa = 10.7HH300 pKa = 6.2FKK302 pKa = 10.7EE303 pKa = 4.46FMGAQRR309 pKa = 11.84DD310 pKa = 3.81WQSNTKK316 pKa = 9.26YY317 pKa = 10.63GKK319 pKa = 9.49PIQIKK324 pKa = 10.42GGIPTIFLCNPGPTSSYY341 pKa = 11.0KK342 pKa = 10.54EE343 pKa = 3.87YY344 pKa = 11.21LEE346 pKa = 4.17EE347 pKa = 5.59DD348 pKa = 3.1KK349 pKa = 11.26NAPLKK354 pKa = 10.76AWATKK359 pKa = 10.05NATFVTLHH367 pKa = 6.54EE368 pKa = 4.54PLFSNTNQGQTPHH381 pKa = 7.48SEE383 pKa = 4.25EE384 pKa = 4.55DD385 pKa = 3.44THH387 pKa = 6.45STT389 pKa = 3.67

Molecular weight: 44.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7DXH5|A7DXH5_9GEMI Replication enhancer OS=Tomato leaf curl Anjouan virus OX=439422 GN=c3 PE=3 SV=1
MM1 pKa = 7.7SKK3 pKa = 10.47RR4 pKa = 11.84PGDD7 pKa = 3.68ILISTPVSKK16 pKa = 10.58VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.29SPFTSRR30 pKa = 11.84AVVPTAPGISRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84AWTYY47 pKa = 9.87RR48 pKa = 11.84PMYY51 pKa = 9.32RR52 pKa = 11.84KK53 pKa = 9.24PKK55 pKa = 9.02IYY57 pKa = 10.46RR58 pKa = 11.84MYY60 pKa = 10.76KK61 pKa = 10.18SPDD64 pKa = 3.3VPKK67 pKa = 10.78GCEE70 pKa = 4.39GPCKK74 pKa = 9.35VQSYY78 pKa = 6.99EE79 pKa = 3.67QRR81 pKa = 11.84DD82 pKa = 4.05DD83 pKa = 3.72VKK85 pKa = 9.5HH86 pKa = 5.48TGIVRR91 pKa = 11.84CVSDD95 pKa = 3.31VTRR98 pKa = 11.84GSGITHH104 pKa = 6.35RR105 pKa = 11.84VGKK108 pKa = 9.68RR109 pKa = 11.84FCIKK113 pKa = 10.32SIYY116 pKa = 9.91FLGKK120 pKa = 8.86IWMDD124 pKa = 3.51EE125 pKa = 4.09NIKK128 pKa = 10.06KK129 pKa = 9.97QNHH132 pKa = 4.95TNQVMFFLVRR142 pKa = 11.84DD143 pKa = 3.69RR144 pKa = 11.84RR145 pKa = 11.84PYY147 pKa = 10.9GSAPMDD153 pKa = 4.11FGQVFNMFDD162 pKa = 3.99NEE164 pKa = 4.11PSTATVKK171 pKa = 10.85NDD173 pKa = 2.82LRR175 pKa = 11.84DD176 pKa = 3.36RR177 pKa = 11.84FQVMRR182 pKa = 11.84KK183 pKa = 7.52FHH185 pKa = 5.82ATVVGGPSGMKK196 pKa = 9.47EE197 pKa = 3.54QALVKK202 pKa = 10.31RR203 pKa = 11.84FFRR206 pKa = 11.84INSHH210 pKa = 3.89VTYY213 pKa = 10.54NHH215 pKa = 5.79QEE217 pKa = 3.49AAKK220 pKa = 10.4YY221 pKa = 9.25EE222 pKa = 4.13NHH224 pKa = 6.46TEE226 pKa = 3.99NALLLYY232 pKa = 7.29MACTHH237 pKa = 7.07ASNPVYY243 pKa = 9.86ATLKK247 pKa = 9.47IRR249 pKa = 11.84IYY251 pKa = 10.67FYY253 pKa = 11.26DD254 pKa = 3.51SVSNN258 pKa = 3.9

Molecular weight: 29.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1109

77

389

184.8

21.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.869 ± 0.977

2.164 ± 0.611

4.959 ± 0.524

4.509 ± 0.613

4.959 ± 0.547

4.779 ± 0.582

4.238 ± 0.6

6.222 ± 0.865

6.312 ± 0.572

7.124 ± 1.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.525 ± 0.488

5.14 ± 0.507

6.132 ± 0.669

4.959 ± 0.711

6.312 ± 1.191

7.845 ± 1.282

5.41 ± 0.217

5.861 ± 1.134

1.262 ± 0.16

4.418 ± 0.461
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski