Amino acid dipepetide frequency for Pichia membranifaciens NRRL Y-2026

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.409AlaAla: 5.409 ± 0.085
0.809AlaCys: 0.809 ± 0.019
3.499AlaAsp: 3.499 ± 0.042
3.893AlaGlu: 3.893 ± 0.051
2.443AlaPhe: 2.443 ± 0.038
3.765AlaGly: 3.765 ± 0.056
1.179AlaHis: 1.179 ± 0.022
3.583AlaIle: 3.583 ± 0.037
4.174AlaLys: 4.174 ± 0.049
5.51AlaLeu: 5.51 ± 0.05
1.233AlaMet: 1.233 ± 0.023
3.156AlaAsn: 3.156 ± 0.041
2.497AlaPro: 2.497 ± 0.047
2.072AlaGln: 2.072 ± 0.038
2.573AlaArg: 2.573 ± 0.035
5.495AlaSer: 5.495 ± 0.053
3.635AlaThr: 3.635 ± 0.045
3.751AlaVal: 3.751 ± 0.048
0.563AlaTrp: 0.563 ± 0.017
1.848AlaTyr: 1.848 ± 0.032
0.0AlaXaa: 0.0 ± 0.0
Cys
0.704CysAla: 0.704 ± 0.016
0.326CysCys: 0.326 ± 0.014
0.718CysAsp: 0.718 ± 0.019
0.649CysGlu: 0.649 ± 0.016
0.732CysPhe: 0.732 ± 0.017
0.872CysGly: 0.872 ± 0.022
0.305CysHis: 0.305 ± 0.012
0.915CysIle: 0.915 ± 0.018
0.728CysLys: 0.728 ± 0.017
1.345CysLeu: 1.345 ± 0.024
0.282CysMet: 0.282 ± 0.01
0.551CysAsn: 0.551 ± 0.016
0.516CysPro: 0.516 ± 0.018
0.387CysGln: 0.387 ± 0.012
0.52CysArg: 0.52 ± 0.015
1.08CysSer: 1.08 ± 0.022
0.661CysThr: 0.661 ± 0.017
0.888CysVal: 0.888 ± 0.02
0.18CysTrp: 0.18 ± 0.009
0.489CysTyr: 0.489 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.654AspAla: 3.654 ± 0.042
0.649AspCys: 0.649 ± 0.017
5.363AspAsp: 5.363 ± 0.085
5.234AspGlu: 5.234 ± 0.061
2.776AspPhe: 2.776 ± 0.032
3.362AspGly: 3.362 ± 0.042
1.11AspHis: 1.11 ± 0.018
3.917AspIle: 3.917 ± 0.046
3.557AspLys: 3.557 ± 0.037
5.729AspLeu: 5.729 ± 0.046
1.234AspMet: 1.234 ± 0.021
3.049AspAsn: 3.049 ± 0.039
2.525AspPro: 2.525 ± 0.032
1.764AspGln: 1.764 ± 0.03
2.249AspArg: 2.249 ± 0.033
5.188AspSer: 5.188 ± 0.056
3.071AspThr: 3.071 ± 0.033
3.748AspVal: 3.748 ± 0.04
0.64AspTrp: 0.64 ± 0.017
2.312AspTyr: 2.312 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
3.689GluAla: 3.689 ± 0.053
0.686GluCys: 0.686 ± 0.017
4.375GluAsp: 4.375 ± 0.046
5.954GluGlu: 5.954 ± 0.093
2.723GluPhe: 2.723 ± 0.035
2.901GluGly: 2.901 ± 0.034
1.217GluHis: 1.217 ± 0.022
4.279GluIle: 4.279 ± 0.046
5.658GluLys: 5.658 ± 0.074
6.332GluLeu: 6.332 ± 0.047
1.461GluMet: 1.461 ± 0.021
4.222GluAsn: 4.222 ± 0.041
1.952GluPro: 1.952 ± 0.026
2.408GluGln: 2.408 ± 0.033
3.001GluArg: 3.001 ± 0.037
4.943GluSer: 4.943 ± 0.058
3.489GluThr: 3.489 ± 0.04
3.535GluVal: 3.535 ± 0.034
0.65GluTrp: 0.65 ± 0.017
2.24GluTyr: 2.24 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
2.535PheAla: 2.535 ± 0.036
0.61PheCys: 0.61 ± 0.016
2.963PheAsp: 2.963 ± 0.032
2.857PheGlu: 2.857 ± 0.037
2.154PhePhe: 2.154 ± 0.036
2.76PheGly: 2.76 ± 0.045
0.95PheHis: 0.95 ± 0.019
2.708PheIle: 2.708 ± 0.037
3.027PheLys: 3.027 ± 0.034
4.067PheLeu: 4.067 ± 0.052
0.909PheMet: 0.909 ± 0.021
2.513PheAsn: 2.513 ± 0.032
1.719PhePro: 1.719 ± 0.025
1.666PheGln: 1.666 ± 0.026
1.737PheArg: 1.737 ± 0.026
3.831PheSer: 3.831 ± 0.047
2.386PheThr: 2.386 ± 0.028
2.823PheVal: 2.823 ± 0.04
0.54PheTrp: 0.54 ± 0.015
1.566PheTyr: 1.566 ± 0.023
0.0PheXaa: 0.0 ± 0.0
Gly
3.491GlyAla: 3.491 ± 0.054
0.813GlyCys: 0.813 ± 0.021
3.196GlyAsp: 3.196 ± 0.036
3.156GlyGlu: 3.156 ± 0.036
2.593GlyPhe: 2.593 ± 0.04
3.874GlyGly: 3.874 ± 0.066
1.15GlyHis: 1.15 ± 0.025
3.352GlyIle: 3.352 ± 0.039
3.81GlyLys: 3.81 ± 0.043
5.022GlyLeu: 5.022 ± 0.048
1.215GlyMet: 1.215 ± 0.027
2.982GlyAsn: 2.982 ± 0.043
1.767GlyPro: 1.767 ± 0.029
1.584GlyGln: 1.584 ± 0.031
2.404GlyArg: 2.404 ± 0.039
4.981GlySer: 4.981 ± 0.054
3.008GlyThr: 3.008 ± 0.04
3.54GlyVal: 3.54 ± 0.043
0.645GlyTrp: 0.645 ± 0.017
2.045GlyTyr: 2.045 ± 0.035
0.0GlyXaa: 0.0 ± 0.0
His
1.142HisAla: 1.142 ± 0.022
0.296HisCys: 0.296 ± 0.012
1.189HisAsp: 1.189 ± 0.022
1.244HisGlu: 1.244 ± 0.023
0.915HisPhe: 0.915 ± 0.02
1.203HisGly: 1.203 ± 0.024
0.679HisHis: 0.679 ± 0.023
1.246HisIle: 1.246 ± 0.024
1.229HisLys: 1.229 ± 0.022
2.0HisLeu: 2.0 ± 0.03
0.441HisMet: 0.441 ± 0.013
1.117HisAsn: 1.117 ± 0.02
1.147HisPro: 1.147 ± 0.022
0.902HisGln: 0.902 ± 0.027
1.019HisArg: 1.019 ± 0.02
1.903HisSer: 1.903 ± 0.029
1.138HisThr: 1.138 ± 0.023
1.219HisVal: 1.219 ± 0.02
0.222HisTrp: 0.222 ± 0.01
0.801HisTyr: 0.801 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
3.474IleAla: 3.474 ± 0.038
0.886IleCys: 0.886 ± 0.019
4.14IleAsp: 4.14 ± 0.043
4.027IleGlu: 4.027 ± 0.05
2.724IlePhe: 2.724 ± 0.038
3.25IleGly: 3.25 ± 0.041
1.298IleHis: 1.298 ± 0.023
3.716IleIle: 3.716 ± 0.045
4.042IleLys: 4.042 ± 0.052
5.882IleLeu: 5.882 ± 0.062
1.287IleMet: 1.287 ± 0.023
3.491IleAsn: 3.491 ± 0.038
3.114IlePro: 3.114 ± 0.034
2.226IleGln: 2.226 ± 0.03
2.589IleArg: 2.589 ± 0.037
5.435IleSer: 5.435 ± 0.048
3.286IleThr: 3.286 ± 0.037
3.863IleVal: 3.863 ± 0.04
0.652IleTrp: 0.652 ± 0.016
2.121IleTyr: 2.121 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
3.857LysAla: 3.857 ± 0.049
0.784LysCys: 0.784 ± 0.021
3.963LysAsp: 3.963 ± 0.038
5.024LysGlu: 5.024 ± 0.06
3.033LysPhe: 3.033 ± 0.034
3.03LysGly: 3.03 ± 0.036
1.479LysHis: 1.479 ± 0.024
4.524LysIle: 4.524 ± 0.053
6.113LysLys: 6.113 ± 0.075
7.145LysLeu: 7.145 ± 0.061
1.506LysMet: 1.506 ± 0.026
4.009LysAsn: 4.009 ± 0.048
2.733LysPro: 2.733 ± 0.041
2.693LysGln: 2.693 ± 0.033
3.611LysArg: 3.611 ± 0.042
5.575LysSer: 5.575 ± 0.057
3.889LysThr: 3.889 ± 0.039
4.122LysVal: 4.122 ± 0.039
0.701LysTrp: 0.701 ± 0.018
2.633LysTyr: 2.633 ± 0.034
0.0LysXaa: 0.0 ± 0.0
Leu
5.737LeuAla: 5.737 ± 0.053
1.274LeuCys: 1.274 ± 0.021
5.722LeuAsp: 5.722 ± 0.047
5.894LeuGlu: 5.894 ± 0.053
4.13LeuPhe: 4.13 ± 0.048
4.785LeuGly: 4.785 ± 0.049
2.031LeuHis: 2.031 ± 0.032
5.644LeuIle: 5.644 ± 0.065
7.255LeuLys: 7.255 ± 0.068
9.406LeuLeu: 9.406 ± 0.087
2.016LeuMet: 2.016 ± 0.029
5.548LeuAsn: 5.548 ± 0.049
4.417LeuPro: 4.417 ± 0.044
3.873LeuGln: 3.873 ± 0.038
4.374LeuArg: 4.374 ± 0.043
8.516LeuSer: 8.516 ± 0.069
5.174LeuThr: 5.174 ± 0.042
5.645LeuVal: 5.645 ± 0.057
0.863LeuTrp: 0.863 ± 0.022
3.023LeuTyr: 3.023 ± 0.036
0.0LeuXaa: 0.0 ± 0.0
Met
1.383MetAla: 1.383 ± 0.022
0.268MetCys: 0.268 ± 0.009
1.252MetAsp: 1.252 ± 0.022
1.245MetGlu: 1.245 ± 0.025
0.911MetPhe: 0.911 ± 0.019
1.16MetGly: 1.16 ± 0.024
0.401MetHis: 0.401 ± 0.015
1.331MetIle: 1.331 ± 0.023
1.586MetLys: 1.586 ± 0.024
1.966MetLeu: 1.966 ± 0.025
0.553MetMet: 0.553 ± 0.014
1.366MetAsn: 1.366 ± 0.033
0.8MetPro: 0.8 ± 0.019
0.75MetGln: 0.75 ± 0.017
0.883MetArg: 0.883 ± 0.019
2.044MetSer: 2.044 ± 0.029
1.132MetThr: 1.132 ± 0.02
1.257MetVal: 1.257 ± 0.025
0.177MetTrp: 0.177 ± 0.008
0.649MetTyr: 0.649 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
3.14AsnAla: 3.14 ± 0.041
0.674AsnCys: 0.674 ± 0.021
3.685AsnAsp: 3.685 ± 0.048
3.731AsnGlu: 3.731 ± 0.037
2.499AsnPhe: 2.499 ± 0.035
3.36AsnGly: 3.36 ± 0.047
1.155AsnHis: 1.155 ± 0.024
3.647AsnIle: 3.647 ± 0.04
3.613AsnLys: 3.613 ± 0.043
5.032AsnLeu: 5.032 ± 0.052
1.274AsnMet: 1.274 ± 0.027
3.949AsnAsn: 3.949 ± 0.075
2.512AsnPro: 2.512 ± 0.037
1.878AsnGln: 1.878 ± 0.037
2.14AsnArg: 2.14 ± 0.03
5.511AsnSer: 5.511 ± 0.065
3.197AsnThr: 3.197 ± 0.044
3.235AsnVal: 3.235 ± 0.035
0.576AsnTrp: 0.576 ± 0.014
2.091AsnTyr: 2.091 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
2.999ProAla: 2.999 ± 0.051
0.374ProCys: 0.374 ± 0.013
2.281ProAsp: 2.281 ± 0.032
2.922ProGlu: 2.922 ± 0.04
1.834ProPhe: 1.834 ± 0.028
2.116ProGly: 2.116 ± 0.03
0.928ProHis: 0.928 ± 0.02
2.533ProIle: 2.533 ± 0.033
2.667ProLys: 2.667 ± 0.035
3.978ProLeu: 3.978 ± 0.041
0.782ProMet: 0.782 ± 0.017
2.267ProAsn: 2.267 ± 0.034
2.425ProPro: 2.425 ± 0.064
1.846ProGln: 1.846 ± 0.042
1.645ProArg: 1.645 ± 0.025
4.063ProSer: 4.063 ± 0.056
2.656ProThr: 2.656 ± 0.04
2.735ProVal: 2.735 ± 0.031
0.382ProTrp: 0.382 ± 0.012
1.388ProTyr: 1.388 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
2.077GlnAla: 2.077 ± 0.041
0.409GlnCys: 0.409 ± 0.013
1.706GlnAsp: 1.706 ± 0.028
2.158GlnGlu: 2.158 ± 0.031
1.607GlnPhe: 1.607 ± 0.026
1.625GlnGly: 1.625 ± 0.03
0.979GlnHis: 0.979 ± 0.031
2.341GlnIle: 2.341 ± 0.03
2.563GlnLys: 2.563 ± 0.031
3.972GlnLeu: 3.972 ± 0.041
0.907GlnMet: 0.907 ± 0.025
2.055GlnAsn: 2.055 ± 0.032
1.664GlnPro: 1.664 ± 0.044
3.02GlnGln: 3.02 ± 0.126
1.848GlnArg: 1.848 ± 0.029
2.837GlnSer: 2.837 ± 0.036
1.938GlnThr: 1.938 ± 0.029
1.954GlnVal: 1.954 ± 0.03
0.346GlnTrp: 0.346 ± 0.012
1.327GlnTyr: 1.327 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
2.548ArgAla: 2.548 ± 0.037
0.589ArgCys: 0.589 ± 0.016
2.258ArgAsp: 2.258 ± 0.032
2.866ArgGlu: 2.866 ± 0.04
1.939ArgPhe: 1.939 ± 0.028
2.356ArgGly: 2.356 ± 0.035
1.027ArgHis: 1.027 ± 0.022
2.644ArgIle: 2.644 ± 0.027
3.494ArgLys: 3.494 ± 0.041
4.347ArgLeu: 4.347 ± 0.04
0.975ArgMet: 0.975 ± 0.019
2.392ArgAsn: 2.392 ± 0.032
1.692ArgPro: 1.692 ± 0.026
1.704ArgGln: 1.704 ± 0.026
2.903ArgArg: 2.903 ± 0.045
3.574ArgSer: 3.574 ± 0.044
2.206ArgThr: 2.206 ± 0.033
2.529ArgVal: 2.529 ± 0.031
0.507ArgTrp: 0.507 ± 0.016
1.53ArgTyr: 1.53 ± 0.027
0.0ArgXaa: 0.0 ± 0.0
Ser
5.315SerAla: 5.315 ± 0.056
1.035SerCys: 1.035 ± 0.021
4.892SerAsp: 4.892 ± 0.051
4.843SerGlu: 4.843 ± 0.047
3.964SerPhe: 3.964 ± 0.045
4.936SerGly: 4.936 ± 0.06
1.823SerHis: 1.823 ± 0.03
5.514SerIle: 5.514 ± 0.059
6.257SerLys: 6.257 ± 0.055
8.229SerLeu: 8.229 ± 0.072
1.829SerMet: 1.829 ± 0.026
5.52SerAsn: 5.52 ± 0.065
3.857SerPro: 3.857 ± 0.063
3.073SerGln: 3.073 ± 0.044
3.742SerArg: 3.742 ± 0.048
10.462SerSer: 10.462 ± 0.133
5.607SerThr: 5.607 ± 0.065
5.084SerVal: 5.084 ± 0.057
0.8SerTrp: 0.8 ± 0.018
2.79SerTyr: 2.79 ± 0.041
0.0SerXaa: 0.0 ± 0.0
Thr
3.694ThrAla: 3.694 ± 0.042
0.702ThrCys: 0.702 ± 0.019
3.069ThrAsp: 3.069 ± 0.038
3.241ThrGlu: 3.241 ± 0.036
2.441ThrPhe: 2.441 ± 0.035
3.322ThrGly: 3.322 ± 0.048
1.171ThrHis: 1.171 ± 0.024
3.439ThrIle: 3.439 ± 0.034
3.606ThrLys: 3.606 ± 0.041
5.216ThrLeu: 5.216 ± 0.043
1.085ThrMet: 1.085 ± 0.018
3.031ThrAsn: 3.031 ± 0.036
2.992ThrPro: 2.992 ± 0.042
1.804ThrGln: 1.804 ± 0.028
2.251ThrArg: 2.251 ± 0.029
5.185ThrSer: 5.185 ± 0.066
3.645ThrThr: 3.645 ± 0.065
3.437ThrVal: 3.437 ± 0.041
0.51ThrTrp: 0.51 ± 0.013
1.71ThrTyr: 1.71 ± 0.024
0.0ThrXaa: 0.0 ± 0.0
Val
3.764ValAla: 3.764 ± 0.046
0.904ValCys: 0.904 ± 0.02
3.975ValAsp: 3.975 ± 0.044
4.042ValGlu: 4.042 ± 0.044
2.722ValPhe: 2.722 ± 0.039
3.426ValGly: 3.426 ± 0.04
1.204ValHis: 1.204 ± 0.023
3.448ValIle: 3.448 ± 0.041
4.028ValLys: 4.028 ± 0.04
5.794ValLeu: 5.794 ± 0.048
1.163ValMet: 1.163 ± 0.02
3.088ValAsn: 3.088 ± 0.034
2.771ValPro: 2.771 ± 0.034
2.08ValGln: 2.08 ± 0.029
2.558ValArg: 2.558 ± 0.031
5.295ValSer: 5.295 ± 0.038
3.057ValThr: 3.057 ± 0.037
3.991ValVal: 3.991 ± 0.045
0.646ValTrp: 0.646 ± 0.016
2.07ValTyr: 2.07 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
0.529TrpAla: 0.529 ± 0.017
0.233TrpCys: 0.233 ± 0.01
0.628TrpAsp: 0.628 ± 0.017
0.612TrpGlu: 0.612 ± 0.016
0.51TrpPhe: 0.51 ± 0.017
0.533TrpGly: 0.533 ± 0.014
0.188TrpHis: 0.188 ± 0.009
0.671TrpIle: 0.671 ± 0.018
0.847TrpLys: 0.847 ± 0.019
0.925TrpLeu: 0.925 ± 0.02
0.245TrpMet: 0.245 ± 0.008
0.635TrpAsn: 0.635 ± 0.016
0.301TrpPro: 0.301 ± 0.012
0.3TrpGln: 0.3 ± 0.011
0.552TrpArg: 0.552 ± 0.016
0.807TrpSer: 0.807 ± 0.019
0.522TrpThr: 0.522 ± 0.014
0.61TrpVal: 0.61 ± 0.017
0.165TrpTrp: 0.165 ± 0.008
0.357TrpTyr: 0.357 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.942TyrAla: 1.942 ± 0.029
0.524TyrCys: 0.524 ± 0.014
2.257TyrAsp: 2.257 ± 0.035
2.124TyrGlu: 2.124 ± 0.028
1.672TyrPhe: 1.672 ± 0.028
1.994TyrGly: 1.994 ± 0.031
0.778TyrHis: 0.778 ± 0.016
2.049TyrIle: 2.049 ± 0.034
2.172TyrLys: 2.172 ± 0.028
3.431TyrLeu: 3.431 ± 0.04
0.716TyrMet: 0.716 ± 0.016
1.956TyrAsn: 1.956 ± 0.031
1.42TyrPro: 1.42 ± 0.024
1.3TyrGln: 1.3 ± 0.025
1.482TyrArg: 1.482 ± 0.023
2.853TyrSer: 2.853 ± 0.041
1.853TyrThr: 1.853 ± 0.026
2.058TyrVal: 2.058 ± 0.032
0.409TyrTrp: 0.409 ± 0.014
1.418TyrTyr: 1.418 ± 0.03
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5536 proteins (2673506 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski