Pontibaca methylaminivorans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Pontibaca

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2497 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R3WYW4|A0A1R3WYW4_9RHOB Glycosyl transferase family 25 OS=Pontibaca methylaminivorans OX=515897 GN=SAMN05421849_1883 PE=4 SV=1
MM1 pKa = 7.16TRR3 pKa = 11.84MLTTLAAAGALALAAGAASAEE24 pKa = 4.32DD25 pKa = 3.93GTWTVEE31 pKa = 3.97FMPMNADD38 pKa = 3.54TAGSEE43 pKa = 4.26AAGTATLTAAGDD55 pKa = 3.78TLTIRR60 pKa = 11.84VDD62 pKa = 3.46ATGTTPGIVHH72 pKa = 6.85LQHH75 pKa = 6.12FHH77 pKa = 6.19GFAEE81 pKa = 4.57GDD83 pKa = 3.64EE84 pKa = 4.85SSTCPGLDD92 pKa = 3.97ADD94 pKa = 4.76TNDD97 pKa = 4.9DD98 pKa = 4.48GIIDD102 pKa = 5.22LIEE105 pKa = 4.19TEE107 pKa = 4.3PAAGTTMVPFHH118 pKa = 7.22GDD120 pKa = 3.39PASMAIEE127 pKa = 4.14AEE129 pKa = 4.65GYY131 pKa = 7.02PTADD135 pKa = 3.54DD136 pKa = 3.75EE137 pKa = 4.69GAYY140 pKa = 10.24SYY142 pKa = 9.95EE143 pKa = 4.33QEE145 pKa = 4.16VSLPDD150 pKa = 3.61LEE152 pKa = 4.85AAFAEE157 pKa = 4.38AFPGQEE163 pKa = 3.6LDD165 pKa = 3.35FGRR168 pKa = 11.84RR169 pKa = 11.84VVFLHH174 pKa = 6.48GVAEE178 pKa = 4.56GTDD181 pKa = 3.74LPDD184 pKa = 3.66TVQSLGDD191 pKa = 3.66VPAHH195 pKa = 4.83VTLPIACGVLEE206 pKa = 4.48AAAEE210 pKa = 4.03

Molecular weight: 21.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R3WEX8|A0A1R3WEX8_9RHOB Uncharacterized protein OS=Pontibaca methylaminivorans OX=515897 GN=SAMN05421849_0523 PE=4 SV=1
MM1 pKa = 7.14NRR3 pKa = 11.84LVTMILRR10 pKa = 11.84IALRR14 pKa = 11.84RR15 pKa = 11.84LVSSGVNHH23 pKa = 7.91GIRR26 pKa = 11.84AAASRR31 pKa = 11.84GGRR34 pKa = 11.84AAANDD39 pKa = 3.61VTRR42 pKa = 11.84GRR44 pKa = 11.84AGGAFGNQRR53 pKa = 11.84RR54 pKa = 11.84TMRR57 pKa = 11.84LLRR60 pKa = 11.84LLRR63 pKa = 11.84RR64 pKa = 3.9

Molecular weight: 7.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2497

0

2497

785412

25

3159

314.5

34.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.925 ± 0.071

0.858 ± 0.016

6.031 ± 0.042

6.155 ± 0.041

3.489 ± 0.032

9.032 ± 0.047

2.146 ± 0.023

5.025 ± 0.033

2.324 ± 0.036

10.382 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.652 ± 0.025

2.282 ± 0.026

5.428 ± 0.032

2.925 ± 0.025

8.201 ± 0.045

4.919 ± 0.03

4.925 ± 0.035

6.892 ± 0.043

1.374 ± 0.023

2.034 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski