Streptomyces sp. Act143

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8401 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U2ZUT0|A0A2U2ZUT0_9ACTN Uncharacterized protein OS=Streptomyces sp. Act143 OX=2200760 GN=DI272_18970 PE=4 SV=1
MM1 pKa = 7.05TAGPGVTVDD10 pKa = 3.93GSGSPSSPYY19 pKa = 10.45VISSDD24 pKa = 3.36AAATPTPVQAGDD36 pKa = 3.66TPTVDD41 pKa = 3.38TTVSGTGTAGDD52 pKa = 4.31PYY54 pKa = 10.56VVSAAVILDD63 pKa = 3.86PAPPGGGSNLVHH75 pKa = 6.85SGPDD79 pKa = 3.44GLFVEE84 pKa = 5.06CADD87 pKa = 4.39VRR89 pKa = 11.84GCLSAGDD96 pKa = 4.31GIGYY100 pKa = 10.02DD101 pKa = 3.49PATGVIEE108 pKa = 4.07AQISGDD114 pKa = 3.57AGNQTTIGTDD124 pKa = 2.93GGLYY128 pKa = 9.84TPVAGATALEE138 pKa = 4.49ATDD141 pKa = 3.97TPTVDD146 pKa = 3.21MTVTGTGTAGDD157 pKa = 4.54PYY159 pKa = 10.72DD160 pKa = 3.69VSAAVIVDD168 pKa = 4.02PAPPGGGPNLLQAGPDD184 pKa = 3.54GLFAEE189 pKa = 5.12IATEE193 pKa = 4.35CGLTGDD199 pKa = 4.75GSAGAPLAASVGAWGYY215 pKa = 10.11PCDD218 pKa = 4.92LDD220 pKa = 3.96TEE222 pKa = 4.61AGQVYY227 pKa = 10.25CDD229 pKa = 3.1STGQLRR235 pKa = 11.84SEE237 pKa = 3.94PRR239 pKa = 11.84PRR241 pKa = 11.84ATYY244 pKa = 10.7LSDD247 pKa = 5.33LVDD250 pKa = 3.19QAYY253 pKa = 9.25PSLIVPTVTEE263 pKa = 4.03ATVVTRR269 pKa = 11.84SLSITNPDD277 pKa = 3.42PCRR280 pKa = 11.84DD281 pKa = 3.1AFVIVEE287 pKa = 4.14IEE289 pKa = 3.86VDD291 pKa = 3.08VDD293 pKa = 3.96LRR295 pKa = 11.84LPVNATAMYY304 pKa = 10.75GMGGDD309 pKa = 3.34DD310 pKa = 2.75TVYY313 pKa = 10.78FEE315 pKa = 4.71NRR317 pKa = 11.84GNSVQNNIHH326 pKa = 5.55WQTTKK331 pKa = 10.67AYY333 pKa = 9.66HH334 pKa = 5.45RR335 pKa = 11.84TIAPGGTLVEE345 pKa = 4.32PLPVTMGLGSNGATYY360 pKa = 10.74QRR362 pKa = 11.84IQTIIRR368 pKa = 11.84AFVFNLL374 pKa = 3.27

Molecular weight: 37.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U2ZWP6|A0A2U2ZWP6_9ACTN DUF2252 domain-containing protein OS=Streptomyces sp. Act143 OX=2200760 GN=DI272_23040 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.46GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8401

0

8401

2773813

18

6019

330.2

35.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.548 ± 0.042

0.788 ± 0.008

6.027 ± 0.023

5.649 ± 0.026

2.742 ± 0.015

9.397 ± 0.03

2.309 ± 0.015

3.121 ± 0.018

2.248 ± 0.02

10.353 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.71 ± 0.011

1.822 ± 0.015

5.99 ± 0.023

2.793 ± 0.015

7.855 ± 0.032

5.049 ± 0.02

6.31 ± 0.027

8.535 ± 0.024

1.576 ± 0.012

2.177 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski