Mariniflexile fucanivorans

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Mariniflexile

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3991 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R1R972|A0A4R1R972_9FLAO Thiol peroxidase OS=Mariniflexile fucanivorans OX=264023 GN=tpx PE=3 SV=1
MM1 pKa = 7.54NLRR4 pKa = 11.84KK5 pKa = 9.85LSLYY9 pKa = 9.92LLCLTIGIVSCKK21 pKa = 10.04KK22 pKa = 10.52DD23 pKa = 3.71DD24 pKa = 4.61DD25 pKa = 4.03PTTVLVEE32 pKa = 3.85IEE34 pKa = 4.47DD35 pKa = 4.57RR36 pKa = 11.84DD37 pKa = 4.02VQQLKK42 pKa = 11.21DD43 pKa = 3.43MDD45 pKa = 4.64SLNKK49 pKa = 10.44YY50 pKa = 10.61LDD52 pKa = 3.23THH54 pKa = 6.69YY55 pKa = 11.37YY56 pKa = 9.89NSEE59 pKa = 3.84EE60 pKa = 4.06LASLGTNVSISDD72 pKa = 3.53IVIGEE77 pKa = 4.2LGEE80 pKa = 4.73DD81 pKa = 3.57EE82 pKa = 4.65TVADD86 pKa = 3.78IPVGFTLLRR95 pKa = 11.84TAVGAPKK102 pKa = 8.48TTTYY106 pKa = 11.57ADD108 pKa = 3.04TGYY111 pKa = 10.71EE112 pKa = 4.18YY113 pKa = 11.42YY114 pKa = 10.66VLKK117 pKa = 10.71LNQGGGSDD125 pKa = 3.78SPKK128 pKa = 9.08FCDD131 pKa = 3.59RR132 pKa = 11.84IRR134 pKa = 11.84FNYY137 pKa = 10.13EE138 pKa = 3.34GFTLDD143 pKa = 5.45DD144 pKa = 3.62VVFDD148 pKa = 4.19YY149 pKa = 10.97SVHH152 pKa = 6.81PIDD155 pKa = 5.17SDD157 pKa = 4.1LVGDD161 pKa = 4.71GGVTSYY167 pKa = 11.9GLITGWNKK175 pKa = 9.88VMPDD179 pKa = 3.41FNTAEE184 pKa = 4.57GYY186 pKa = 9.84IEE188 pKa = 5.03NGDD191 pKa = 3.68GTVTYY196 pKa = 10.28VNKK199 pKa = 10.52GVGIMFLPSGLAYY212 pKa = 10.45FSSATTGIVSYY223 pKa = 11.0SPIIFKK229 pKa = 10.92FEE231 pKa = 4.2LLQMSQNDD239 pKa = 3.62HH240 pKa = 7.36DD241 pKa = 5.11GDD243 pKa = 4.25GVPSYY248 pKa = 11.42LEE250 pKa = 4.73DD251 pKa = 3.85LPNSNGVRR259 pKa = 11.84DD260 pKa = 3.75SQFTVNYY267 pKa = 10.25DD268 pKa = 3.78DD269 pKa = 5.34LTDD272 pKa = 3.94ATDD275 pKa = 4.96DD276 pKa = 4.03DD277 pKa = 4.58TDD279 pKa = 4.57GDD281 pKa = 4.17GTPDD285 pKa = 4.8YY286 pKa = 10.97IDD288 pKa = 4.15TDD290 pKa = 4.08DD291 pKa = 5.43DD292 pKa = 4.89GDD294 pKa = 4.44GISTINEE301 pKa = 4.68DD302 pKa = 3.78INNDD306 pKa = 3.47GDD308 pKa = 4.04PTNDD312 pKa = 3.51DD313 pKa = 4.02SNGNGIPNYY322 pKa = 10.38LDD324 pKa = 3.03NTDD327 pKa = 3.98IIKK330 pKa = 10.62KK331 pKa = 8.05VTTT334 pKa = 3.81

Molecular weight: 36.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V2QEQ5|A0A4V2QEQ5_9FLAO TatD DNase family protein OS=Mariniflexile fucanivorans OX=264023 GN=EV196_101374 PE=4 SV=1
MM1 pKa = 7.22KK2 pKa = 10.2RR3 pKa = 11.84ISPRR7 pKa = 11.84MRR9 pKa = 11.84FRR11 pKa = 11.84MMNPFLQFFKK21 pKa = 10.91FIILSIKK28 pKa = 10.26IMVIVAGGHH37 pKa = 5.35GGTRR41 pKa = 11.84KK42 pKa = 10.18VNN44 pKa = 3.26

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3991

0

3991

1414510

30

4036

354.4

39.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.164 ± 0.038

0.788 ± 0.012

5.579 ± 0.029

6.335 ± 0.038

5.25 ± 0.029

6.303 ± 0.039

1.767 ± 0.02

8.131 ± 0.037

7.981 ± 0.055

9.152 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.131 ± 0.019

6.743 ± 0.043

3.363 ± 0.022

3.215 ± 0.02

3.093 ± 0.023

6.573 ± 0.033

6.075 ± 0.055

6.106 ± 0.031

1.087 ± 0.014

4.165 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski