Cronobacter phage vB_CsaP_GAP52

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 115 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4F6W0|K4F6W0_9CAUD Uncharacterized protein OS=Cronobacter phage vB_CsaP_GAP52 OX=1141137 GN=GAP52_053 PE=4 SV=1
MM1 pKa = 7.34QYY3 pKa = 10.33VDD5 pKa = 5.17KK6 pKa = 11.48YY7 pKa = 11.37SGDD10 pKa = 3.76DD11 pKa = 3.48VMKK14 pKa = 10.66GDD16 pKa = 3.61EE17 pKa = 4.31ALFIFKK23 pKa = 9.43TDD25 pKa = 4.29CLDD28 pKa = 3.24QGQLINFTEE37 pKa = 4.07LANLKK42 pKa = 10.48EE43 pKa = 4.6EE44 pKa = 4.11IDD46 pKa = 3.45RR47 pKa = 11.84LYY49 pKa = 11.24GDD51 pKa = 3.66EE52 pKa = 4.01QSRR55 pKa = 11.84ILNEE59 pKa = 4.15SLLGDD64 pKa = 4.02YY65 pKa = 10.14TCDD68 pKa = 2.97GCTII72 pKa = 4.06

Molecular weight: 8.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4F7D2|K4F7D2_9CAUD Uncharacterized protein OS=Cronobacter phage vB_CsaP_GAP52 OX=1141137 GN=GAP52_001 PE=4 SV=1
MM1 pKa = 7.21SKK3 pKa = 10.09KK4 pKa = 10.05RR5 pKa = 11.84AIGRR9 pKa = 11.84MIHH12 pKa = 6.46NGVQINKK19 pKa = 9.5LVTPNMVKK27 pKa = 10.38AIKK30 pKa = 10.09RR31 pKa = 11.84RR32 pKa = 11.84EE33 pKa = 3.98NLVKK37 pKa = 10.37EE38 pKa = 4.34LNTVIDD44 pKa = 3.96NVDD47 pKa = 2.88KK48 pKa = 11.14RR49 pKa = 11.84FTFEE53 pKa = 3.4QVMRR57 pKa = 11.84AKK59 pKa = 9.52TALRR63 pKa = 11.84KK64 pKa = 9.92LYY66 pKa = 9.97AQSHH70 pKa = 5.98ALDD73 pKa = 4.39EE74 pKa = 5.08KK75 pKa = 11.02PLTNNKK81 pKa = 9.98DD82 pKa = 3.19SWCAKK87 pKa = 7.82GKK89 pKa = 10.38RR90 pKa = 11.84YY91 pKa = 8.43ATGKK95 pKa = 10.03QKK97 pKa = 10.93AKK99 pKa = 10.31RR100 pKa = 11.84LPSQPRR106 pKa = 11.84VEE108 pKa = 5.03FSRR111 pKa = 11.84KK112 pKa = 7.0SS113 pKa = 3.21

Molecular weight: 13.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

115

0

115

23843

37

1304

207.3

23.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.388 ± 0.477

1.111 ± 0.136

6.325 ± 0.194

6.945 ± 0.307

3.821 ± 0.219

7.298 ± 0.269

2.022 ± 0.137

5.36 ± 0.189

6.56 ± 0.235

7.113 ± 0.201

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.2 ± 0.118

5.176 ± 0.22

3.989 ± 0.169

3.892 ± 0.255

4.945 ± 0.215

6.048 ± 0.196

5.738 ± 0.213

6.744 ± 0.225

1.606 ± 0.156

3.72 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski