Rotavirus A (isolate RVA/Human/United Kingdom/A64/1987/G10P11[14]) (RV-A)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus A; Rotavirus G10; Rotavirus G10P11

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q07272-2|VP7-2_ROTH7 Isoform of Q07272 Isoform 2 of Outer capsid glycoprotein VP7 OS=Rotavirus A (isolate RVA/Human/United Kingdom/A64/1987/G10P11[14]) OX=578827 PE=4 SV=1
MM1 pKa = 8.15DD2 pKa = 4.65YY3 pKa = 10.56IIYY6 pKa = 10.37KK7 pKa = 9.75FLLIITITSIFDD19 pKa = 3.11SAQNYY24 pKa = 8.41GINLPITGSMDD35 pKa = 3.14VSYY38 pKa = 11.94VNATKK43 pKa = 10.55DD44 pKa = 3.69EE45 pKa = 4.52PFLTSTLCLYY55 pKa = 10.79YY56 pKa = 8.97PTEE59 pKa = 4.21ARR61 pKa = 11.84TEE63 pKa = 4.03INDD66 pKa = 3.89NEE68 pKa = 4.33WTSTLSQLFLTKK80 pKa = 10.05GWPTGSVYY88 pKa = 10.44FKK90 pKa = 10.27EE91 pKa = 4.24YY92 pKa = 10.95DD93 pKa = 4.21DD94 pKa = 4.85IPTFSVDD101 pKa = 3.09PQLYY105 pKa = 9.02CDD107 pKa = 3.86YY108 pKa = 10.68NIVLMRR114 pKa = 11.84YY115 pKa = 9.02NSSLKK120 pKa = 10.56LDD122 pKa = 3.61MSEE125 pKa = 4.39LANLILNEE133 pKa = 4.07WLCNPMDD140 pKa = 3.29ITLYY144 pKa = 10.67YY145 pKa = 9.25YY146 pKa = 10.55QQTDD150 pKa = 3.48EE151 pKa = 4.21ANKK154 pKa = 8.63WIAMGQSCTIKK165 pKa = 10.31VCPLNTQTLGIGCQTTNTGTFEE187 pKa = 4.07EE188 pKa = 4.95VATAEE193 pKa = 4.34KK194 pKa = 10.74LVITDD199 pKa = 3.22VVDD202 pKa = 4.31GVNHH206 pKa = 6.65KK207 pKa = 10.84LDD209 pKa = 3.31VTTASRR215 pKa = 11.84TIRR218 pKa = 11.84NCKK221 pKa = 9.75KK222 pKa = 10.13LGPRR226 pKa = 11.84EE227 pKa = 3.99NVAVIQIGGADD238 pKa = 3.39ILDD241 pKa = 3.75ITSDD245 pKa = 3.59PTTTPQTEE253 pKa = 3.42RR254 pKa = 11.84MMRR257 pKa = 11.84INWKK261 pKa = 9.54KK262 pKa = 7.26WWQVFYY268 pKa = 10.66TIVDD272 pKa = 3.57YY273 pKa = 11.53VNQIVQTMSKK283 pKa = 10.13RR284 pKa = 11.84SRR286 pKa = 11.84SLDD289 pKa = 3.17SAAFYY294 pKa = 11.19YY295 pKa = 10.29RR296 pKa = 11.84VV297 pKa = 3.19

Molecular weight: 33.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P30031|NSP4_ROTH7 Non-structural glycoprotein 4 OS=Rotavirus A (isolate RVA/Human/United Kingdom/A64/1987/G10P11[14]) OX=578827 PE=3 SV=1
MM1 pKa = 7.27NHH3 pKa = 6.21LQQRR7 pKa = 11.84QLFLEE12 pKa = 4.16NLLVGVNNTFHH23 pKa = 6.59QMQKK27 pKa = 10.75HH28 pKa = 6.38SINTCCQSLQKK39 pKa = 10.62ILDD42 pKa = 3.85HH43 pKa = 7.17LILLQTIHH51 pKa = 6.63SPAFRR56 pKa = 11.84LDD58 pKa = 3.35RR59 pKa = 11.84MQLRR63 pKa = 11.84QMQTLACLWIHH74 pKa = 5.64QHH76 pKa = 5.12NHH78 pKa = 4.9DD79 pKa = 4.04HH80 pKa = 6.06QAMLGAIKK88 pKa = 9.91WISPLIKK95 pKa = 10.06EE96 pKa = 4.4LRR98 pKa = 3.73

Molecular weight: 11.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

1

13

6187

98

1088

475.9

54.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.124 ± 0.323

1.261 ± 0.281

6.045 ± 0.253

5.495 ± 0.293

4.186 ± 0.247

3.41 ± 0.284

1.552 ± 0.311

7.597 ± 0.366

6.223 ± 0.548

9.326 ± 0.444

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.216 ± 0.233

7.112 ± 0.314

3.152 ± 0.213

4.348 ± 0.451

4.897 ± 0.262

7.791 ± 0.806

6.772 ± 0.613

6.11 ± 0.212

1.196 ± 0.134

5.188 ± 0.475
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski