Bifidobacterium animalis subsp. lactis (strain AD011)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium; Bifidobacterium animalis; Bifidobacterium animalis subsp. lactis

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1525 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8DTC1|B8DTC1_BIFA0 Peptidyl-prolyl cis-trans isomerase OS=Bifidobacterium animalis subsp. lactis (strain AD011) OX=442563 GN=BLA_0960 PE=3 SV=1
MM1 pKa = 7.13AQDD4 pKa = 3.56YY5 pKa = 10.0DD6 pKa = 3.94SPRR9 pKa = 11.84SKK11 pKa = 11.34DD12 pKa = 3.26EE13 pKa = 4.8DD14 pKa = 3.97EE15 pKa = 5.29EE16 pKa = 4.46SLQALGKK23 pKa = 10.18SSQNASNDD31 pKa = 3.12MDD33 pKa = 5.09DD34 pKa = 4.97DD35 pKa = 4.34EE36 pKa = 4.86NAIAEE41 pKa = 4.35DD42 pKa = 4.23YY43 pKa = 9.11EE44 pKa = 5.05LPGADD49 pKa = 5.03LSNEE53 pKa = 4.1DD54 pKa = 3.77ASVTVIPMQGDD65 pKa = 3.48EE66 pKa = 5.44FICSEE71 pKa = 4.23CFLVKK76 pKa = 10.28HH77 pKa = 6.48RR78 pKa = 11.84SQLAYY83 pKa = 6.59TTPDD87 pKa = 3.21GQPVCKK93 pKa = 10.14EE94 pKa = 3.94CAAA97 pKa = 4.21

Molecular weight: 10.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8DV37|B8DV37_BIFA0 Uncharacterized protein OS=Bifidobacterium animalis subsp. lactis (strain AD011) OX=442563 GN=BLA_0033 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.79MKK15 pKa = 9.29SGFRR19 pKa = 11.84VRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84AGRR28 pKa = 11.84ALINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.08GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1525

0

1525

542643

29

3105

355.8

39.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.653 ± 0.079

1.01 ± 0.023

6.464 ± 0.059

6.079 ± 0.071

3.471 ± 0.036

7.762 ± 0.053

2.414 ± 0.029

5.39 ± 0.047

3.631 ± 0.055

8.787 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.839 ± 0.031

3.485 ± 0.04

4.686 ± 0.038

3.656 ± 0.033

6.17 ± 0.068

5.825 ± 0.044

5.862 ± 0.059

7.78 ± 0.059

1.326 ± 0.021

2.71 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski