Salmonella phage SPC32N

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Uetakevirus; Salmonella virus SPN1S

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T1SBJ1|T1SBJ1_9CAUD Putative structural protein OS=Salmonella phage SPC32N OX=1327942 GN=SPC32N_016 PE=4 SV=1
MM1 pKa = 7.04KK2 pKa = 9.48TVNMKK7 pKa = 9.57TGTDD11 pKa = 3.47SFVGEE16 pKa = 4.34DD17 pKa = 4.08GKK19 pKa = 10.18PEE21 pKa = 4.15TKK23 pKa = 10.27DD24 pKa = 3.14QYY26 pKa = 11.07PWGLRR31 pKa = 11.84ITLDD35 pKa = 3.44NEE37 pKa = 4.1SLQRR41 pKa = 11.84LGLNAKK47 pKa = 9.35SLPAVGDD54 pKa = 4.16SVSVMAMASVCSVSTRR70 pKa = 11.84TTDD73 pKa = 2.85HH74 pKa = 6.84GEE76 pKa = 3.81DD77 pKa = 3.95SYY79 pKa = 12.32VEE81 pKa = 3.96LQITDD86 pKa = 3.17IGLAPQKK93 pKa = 10.57RR94 pKa = 11.84DD95 pKa = 3.42DD96 pKa = 3.98AKK98 pKa = 10.15EE99 pKa = 4.01LKK101 pKa = 10.45DD102 pKa = 3.15AFYY105 pKa = 10.36PVGEE109 pKa = 4.87DD110 pKa = 3.9DD111 pKa = 4.13

Molecular weight: 12.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T1S9J7|T1S9J7_9CAUD Putative exonuclease VIII/RecE-like protein OS=Salmonella phage SPC32N OX=1327942 GN=SPC32N_038 PE=4 SV=1
MM1 pKa = 7.69VFIPDD6 pKa = 3.05RR7 pKa = 11.84RR8 pKa = 11.84FRR10 pKa = 11.84LAVSAGGNFQRR21 pKa = 11.84RR22 pKa = 11.84LDD24 pKa = 3.94PMSKK28 pKa = 8.89LTRR31 pKa = 11.84LEE33 pKa = 4.39KK34 pKa = 10.42YY35 pKa = 9.09HH36 pKa = 6.74LNYY39 pKa = 10.05VSQRR43 pKa = 11.84QASKK47 pKa = 10.96VVAVTPAAMEE57 pKa = 4.06VEE59 pKa = 4.0KK60 pKa = 10.77RR61 pKa = 11.84AVEE64 pKa = 4.11RR65 pKa = 11.84EE66 pKa = 4.02SKK68 pKa = 9.44GQYY71 pKa = 9.79RR72 pKa = 11.84IAARR76 pKa = 11.84LWLLCMDD83 pKa = 4.14VAVGEE88 pKa = 4.29VEE90 pKa = 4.02RR91 pKa = 11.84ARR93 pKa = 11.84IAIRR97 pKa = 11.84RR98 pKa = 11.84DD99 pKa = 3.16QCISKK104 pKa = 10.94GNGLRR109 pKa = 11.84RR110 pKa = 11.84GEE112 pKa = 3.99YY113 pKa = 10.35AGIGCRR119 pKa = 11.84GVVYY123 pKa = 10.43DD124 pKa = 4.23

Molecular weight: 14.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

12028

38

937

235.8

26.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.943 ± 0.614

1.014 ± 0.15

6.077 ± 0.268

5.72 ± 0.349

3.567 ± 0.231

7.416 ± 0.291

1.588 ± 0.163

5.703 ± 0.479

5.205 ± 0.314

7.607 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.777 ± 0.272

4.664 ± 0.242

3.999 ± 0.214

4.814 ± 0.471

5.861 ± 0.361

6.61 ± 0.409

5.836 ± 0.342

6.601 ± 0.284

1.696 ± 0.164

3.301 ± 0.257
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski