Rhizoctonia solani 123E

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Cantharellales; Ceratobasidiaceae; Rhizoctonia; Rhizoctonia solani; Rhizoctonia solani AG-3

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10990 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A074SEL9|A0A074SEL9_9AGAM BTB/POZ domain protein OS=Rhizoctonia solani 123E OX=1423351 GN=V565_123630 PE=4 SV=1
MM1 pKa = 7.58GISYY5 pKa = 10.41YY6 pKa = 10.78DD7 pKa = 3.7EE8 pKa = 5.77IEE10 pKa = 4.5IEE12 pKa = 4.18DD13 pKa = 4.14MSWDD17 pKa = 3.12ADD19 pKa = 3.22KK20 pKa = 11.14RR21 pKa = 11.84VFHH24 pKa = 6.1YY25 pKa = 8.48PCPCGDD31 pKa = 3.22RR32 pKa = 11.84FEE34 pKa = 4.49ISRR37 pKa = 11.84AQLGNYY43 pKa = 8.87EE44 pKa = 4.91DD45 pKa = 4.83VATCPSCSLIIRR57 pKa = 11.84VVYY60 pKa = 10.58DD61 pKa = 3.57PLDD64 pKa = 4.05FEE66 pKa = 5.61DD67 pKa = 5.15EE68 pKa = 4.25EE69 pKa = 4.87EE70 pKa = 4.08EE71 pKa = 4.76DD72 pKa = 4.05EE73 pKa = 4.49EE74 pKa = 4.54EE75 pKa = 4.16EE76 pKa = 4.4SASVEE81 pKa = 3.94EE82 pKa = 5.79DD83 pKa = 3.08KK84 pKa = 11.65LQDD87 pKa = 3.5ALEE90 pKa = 4.26NLTLEE95 pKa = 4.3PTIVSLPARR104 pKa = 11.84TT105 pKa = 3.39

Molecular weight: 12.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A074S7I1|A0A074S7I1_9AGAM PfkB family carbohydrate kinase OS=Rhizoctonia solani 123E OX=1423351 GN=V565_008190 PE=4 SV=1
MM1 pKa = 7.34SKK3 pKa = 10.45SRR5 pKa = 11.84TRR7 pKa = 11.84TVGRR11 pKa = 11.84KK12 pKa = 7.77MKK14 pKa = 10.52QKK16 pKa = 9.34TEE18 pKa = 3.79RR19 pKa = 11.84ARR21 pKa = 11.84GKK23 pKa = 9.13TMEE26 pKa = 4.16KK27 pKa = 9.7RR28 pKa = 11.84KK29 pKa = 9.27QGRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84GAKK37 pKa = 9.7LGPSPRR43 pKa = 11.84AEE45 pKa = 3.65RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84SGQSSRR54 pKa = 11.84PKK56 pKa = 9.25SRR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84MRR63 pKa = 11.84WRR65 pKa = 11.84KK66 pKa = 7.85RR67 pKa = 11.84TRR69 pKa = 11.84SWCRR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84GTRR79 pKa = 11.84PARR82 pKa = 11.84NPRR85 pKa = 11.84SRR87 pKa = 11.84RR88 pKa = 11.84SRR90 pKa = 11.84RR91 pKa = 11.84SWGRR95 pKa = 11.84SRR97 pKa = 11.84SRR99 pKa = 11.84DD100 pKa = 3.12EE101 pKa = 3.76RR102 pKa = 11.84LGRR105 pKa = 11.84NRR107 pKa = 11.84MSRR110 pKa = 11.84KK111 pKa = 9.27RR112 pKa = 3.51

Molecular weight: 13.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10990

0

10990

5600857

10

7806

509.6

56.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.567 ± 0.024

1.249 ± 0.01

5.439 ± 0.015

5.794 ± 0.02

3.527 ± 0.012

6.745 ± 0.018

2.586 ± 0.009

4.969 ± 0.016

4.468 ± 0.021

9.103 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.083 ± 0.008

3.605 ± 0.014

6.569 ± 0.03

3.794 ± 0.014

6.148 ± 0.02

8.555 ± 0.029

6.245 ± 0.013

6.308 ± 0.017

1.475 ± 0.008

2.77 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski