Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanomicrobiales; Methanoregulaceae; Methanosphaerula; Methanosphaerula palustris

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2655 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8GHQ2|B8GHQ2_METPE Metallophosphoesterase OS=Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c) OX=521011 GN=Mpal_1324 PE=4 SV=1
MM1 pKa = 8.52VLMNPNEE8 pKa = 4.31PDD10 pKa = 3.22TDD12 pKa = 3.42EE13 pKa = 4.85DD14 pKa = 3.77QPEE17 pKa = 4.12EE18 pKa = 4.25EE19 pKa = 4.82KK20 pKa = 10.67MVDD23 pKa = 3.54HH24 pKa = 6.36EE25 pKa = 4.85TLLEE29 pKa = 4.03MTADD33 pKa = 5.68DD34 pKa = 5.02IFADD38 pKa = 3.96CEE40 pKa = 4.41DD41 pKa = 4.51DD42 pKa = 4.05VVCIAEE48 pKa = 4.3ALEE51 pKa = 4.52DD52 pKa = 4.56LDD54 pKa = 3.78PAMRR58 pKa = 11.84DD59 pKa = 3.29EE60 pKa = 5.13LLVSDD65 pKa = 5.22HH66 pKa = 6.73LNAYY70 pKa = 6.92QTFYY74 pKa = 11.18YY75 pKa = 10.03YY76 pKa = 10.69FRR78 pKa = 11.84QIPEE82 pKa = 3.77EE83 pKa = 4.5LIQEE87 pKa = 4.19RR88 pKa = 11.84LILQPASALGEE99 pKa = 4.11GVLVEE104 pKa = 5.21EE105 pKa = 4.41IDD107 pKa = 3.91LYY109 pKa = 11.23EE110 pKa = 4.36LVFTVHH116 pKa = 6.3QRR118 pKa = 11.84GPLMAVSDD126 pKa = 4.42GEE128 pKa = 4.2ALLEE132 pKa = 4.31TFEE135 pKa = 5.05GPHH138 pKa = 6.61AYY140 pKa = 8.72TRR142 pKa = 11.84ARR144 pKa = 11.84AYY146 pKa = 10.54AEE148 pKa = 4.11DD149 pKa = 4.88LII151 pKa = 5.91

Molecular weight: 17.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|B8GEU6|IF6_METPE Translation initiation factor 6 OS=Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c) OX=521011 GN=eif6 PE=3 SV=1
MM1 pKa = 7.65SKK3 pKa = 8.86LTKK6 pKa = 9.74GRR8 pKa = 11.84KK9 pKa = 8.66IRR11 pKa = 11.84LSKK14 pKa = 10.13ATAQNRR20 pKa = 11.84RR21 pKa = 11.84VPSWVMIKK29 pKa = 8.99TKK31 pKa = 10.14RR32 pKa = 11.84AVVSHH37 pKa = 6.42PKK39 pKa = 8.99RR40 pKa = 11.84RR41 pKa = 11.84SWRR44 pKa = 11.84RR45 pKa = 11.84SSLKK49 pKa = 10.39VV50 pKa = 3.13

Molecular weight: 5.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2655

0

2655

818093

30

3295

308.1

33.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.528 ± 0.064

1.258 ± 0.021

5.57 ± 0.035

6.077 ± 0.075

3.731 ± 0.034

8.15 ± 0.048

2.023 ± 0.022

7.001 ± 0.064

3.852 ± 0.047

9.449 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.534 ± 0.028

3.45 ± 0.051

4.764 ± 0.039

3.394 ± 0.024

5.564 ± 0.058

6.077 ± 0.046

6.79 ± 0.093

7.757 ± 0.045

1.044 ± 0.02

2.987 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski