Amino acid dipepetide frequency for Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.949AlaAla: 8.949 ± 0.159	1.059AlaCys: 1.059 ± 0.037	4.847AlaAsp: 4.847 ± 0.091	5.271AlaGlu: 5.271 ± 0.096	3.017AlaPhe: 3.017 ± 0.071	8.61AlaGly: 8.61 ± 0.137	1.414AlaHis: 1.414 ± 0.041	5.933AlaIle: 5.933 ± 0.102	2.579AlaLys: 2.579 ± 0.061	8.676AlaLeu: 8.676 ± 0.146	2.324AlaMet: 2.324 ± 0.073	2.406AlaAsn: 2.406 ± 0.076	3.238AlaPro: 3.238 ± 0.084	2.248AlaGln: 2.248 ± 0.056	4.427AlaArg: 4.427 ± 0.083	4.639AlaSer: 4.639 ± 0.091	5.102AlaThr: 5.102 ± 0.108	7.376AlaVal: 7.376 ± 0.11	0.897AlaTrp: 0.897 ± 0.034	2.267AlaTyr: 2.267 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	0.919CysAla: 0.919 ± 0.04	0.247CysCys: 0.247 ± 0.018	0.61CysAsp: 0.61 ± 0.029	0.59CysGlu: 0.59 ± 0.026	0.362CysPhe: 0.362 ± 0.025	1.412CysGly: 1.412 ± 0.047	0.295CysHis: 0.295 ± 0.018	0.803CysIle: 0.803 ± 0.033	0.395CysLys: 0.395 ± 0.021	0.969CysLeu: 0.969 ± 0.038	0.341CysMet: 0.341 ± 0.034	0.392CysAsn: 0.392 ± 0.023	0.863CysPro: 0.863 ± 0.04	0.335CysGln: 0.335 ± 0.022	0.895CysArg: 0.895 ± 0.032	0.881CysSer: 0.881 ± 0.032	0.989CysThr: 0.989 ± 0.038	0.77CysVal: 0.77 ± 0.033	0.154CysTrp: 0.154 ± 0.015	0.357CysTyr: 0.357 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	4.37AspAla: 4.37 ± 0.08	0.677AspCys: 0.677 ± 0.029	2.832AspAsp: 2.832 ± 0.074	3.803AspGlu: 3.803 ± 0.079	1.973AspPhe: 1.973 ± 0.06	4.854AspGly: 4.854 ± 0.091	1.391AspHis: 1.391 ± 0.044	3.49AspIle: 3.49 ± 0.071	1.548AspLys: 1.548 ± 0.05	7.196AspLeu: 7.196 ± 0.09	1.177AspMet: 1.177 ± 0.037	1.467AspAsn: 1.467 ± 0.05	3.295AspPro: 3.295 ± 0.076	2.635AspGln: 2.635 ± 0.055	3.83AspArg: 3.83 ± 0.079	2.491AspSer: 2.491 ± 0.062	2.815AspThr: 2.815 ± 0.064	3.55AspVal: 3.55 ± 0.086	0.68AspTrp: 0.68 ± 0.035	1.623AspTyr: 1.623 ± 0.048	0.0AspXaa: 0.0 ± 0.0
Glu	5.147GluAla: 5.147 ± 0.109	0.562GluCys: 0.562 ± 0.027	2.996GluAsp: 2.996 ± 0.071	5.117GluGlu: 5.117 ± 0.108	1.89GluPhe: 1.89 ± 0.043	4.353GluGly: 4.353 ± 0.074	1.417GluHis: 1.417 ± 0.048	4.519GluIle: 4.519 ± 0.092	3.37GluLys: 3.37 ± 0.089	4.735GluLeu: 4.735 ± 0.099	1.847GluMet: 1.847 ± 0.055	2.003GluAsn: 2.003 ± 0.052	2.32GluPro: 2.32 ± 0.056	2.884GluGln: 2.884 ± 0.066	4.13GluArg: 4.13 ± 0.093	2.996GluSer: 2.996 ± 0.069	3.401GluThr: 3.401 ± 0.067	4.711GluVal: 4.711 ± 0.11	0.564GluTrp: 0.564 ± 0.027	1.81GluTyr: 1.81 ± 0.053	0.0GluXaa: 0.0 ± 0.0
Phe	2.959PheAla: 2.959 ± 0.067	0.513PheCys: 0.513 ± 0.028	2.313PheAsp: 2.313 ± 0.062	1.977PheGlu: 1.977 ± 0.059	1.737PhePhe: 1.737 ± 0.059	3.144PheGly: 3.144 ± 0.068	0.742PheHis: 0.742 ± 0.033	2.524PheIle: 2.524 ± 0.067	1.169PheLys: 1.169 ± 0.039	3.544PheLeu: 3.544 ± 0.088	0.837PheMet: 0.837 ± 0.034	1.576PheAsn: 1.576 ± 0.056	1.578PhePro: 1.578 ± 0.046	1.07PheGln: 1.07 ± 0.034	1.818PheArg: 1.818 ± 0.047	2.961PheSer: 2.961 ± 0.066	2.838PheThr: 2.838 ± 0.08	2.538PheVal: 2.538 ± 0.057	0.483PheTrp: 0.483 ± 0.025	0.994PheTyr: 0.994 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	6.387GlyAla: 6.387 ± 0.097	1.197GlyCys: 1.197 ± 0.04	4.532GlyAsp: 4.532 ± 0.098	4.909GlyGlu: 4.909 ± 0.095	3.273GlyPhe: 3.273 ± 0.072	6.194GlyGly: 6.194 ± 0.103	1.455GlyHis: 1.455 ± 0.046	6.4GlyIle: 6.4 ± 0.096	3.49GlyLys: 3.49 ± 0.08	6.922GlyLeu: 6.922 ± 0.118	2.335GlyMet: 2.335 ± 0.056	2.902GlyAsn: 2.902 ± 0.107	2.767GlyPro: 2.767 ± 0.066	2.402GlyGln: 2.402 ± 0.061	4.393GlyArg: 4.393 ± 0.08	5.529GlySer: 5.529 ± 0.119	6.067GlyThr: 6.067 ± 0.126	6.449GlyVal: 6.449 ± 0.097	0.973GlyTrp: 0.973 ± 0.036	2.926GlyTyr: 2.926 ± 0.068	0.0GlyXaa: 0.0 ± 0.0
His	1.629HisAla: 1.629 ± 0.047	0.376HisCys: 0.376 ± 0.021	1.175HisAsp: 1.175 ± 0.042	1.07HisGlu: 1.07 ± 0.034	0.631HisPhe: 0.631 ± 0.026	1.566HisGly: 1.566 ± 0.047	0.67HisHis: 0.67 ± 0.032	1.205HisIle: 1.205 ± 0.04	0.561HisLys: 0.561 ± 0.026	2.513HisLeu: 2.513 ± 0.055	0.395HisMet: 0.395 ± 0.024	0.583HisAsn: 0.583 ± 0.025	1.44HisPro: 1.44 ± 0.038	0.927HisGln: 0.927 ± 0.033	1.18HisArg: 1.18 ± 0.041	0.986HisSer: 0.986 ± 0.036	1.228HisThr: 1.228 ± 0.05	1.221HisVal: 1.221 ± 0.035	0.232HisTrp: 0.232 ± 0.017	0.644HisTyr: 0.644 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	6.532IleAla: 6.532 ± 0.102	1.018IleCys: 1.018 ± 0.04	4.179IleAsp: 4.179 ± 0.075	4.643IleGlu: 4.643 ± 0.091	2.536IlePhe: 2.536 ± 0.071	5.968IleGly: 5.968 ± 0.112	1.358IleHis: 1.358 ± 0.04	4.196IleIle: 4.196 ± 0.1	2.259IleLys: 2.259 ± 0.063	5.996IleLeu: 5.996 ± 0.113	1.296IleMet: 1.296 ± 0.046	2.228IleAsn: 2.228 ± 0.06	3.898IlePro: 3.898 ± 0.082	2.135IleGln: 2.135 ± 0.061	4.573IleArg: 4.573 ± 0.085	4.654IleSer: 4.654 ± 0.095	5.157IleThr: 5.157 ± 0.091	4.921IleVal: 4.921 ± 0.082	0.672IleTrp: 0.672 ± 0.033	1.791IleTyr: 1.791 ± 0.053	0.0IleXaa: 0.0 ± 0.0
Lys	2.929LysAla: 2.929 ± 0.074	0.351LysCys: 0.351 ± 0.024	2.217LysAsp: 2.217 ± 0.061	2.868LysGlu: 2.868 ± 0.073	1.244LysPhe: 1.244 ± 0.047	3.085LysGly: 3.085 ± 0.064	0.671LysHis: 0.671 ± 0.027	2.959LysIle: 2.959 ± 0.079	2.741LysLys: 2.741 ± 0.08	2.509LysLeu: 2.509 ± 0.067	1.083LysMet: 1.083 ± 0.04	1.863LysAsn: 1.863 ± 0.057	1.563LysPro: 1.563 ± 0.051	1.237LysGln: 1.237 ± 0.042	2.241LysArg: 2.241 ± 0.057	1.986LysSer: 1.986 ± 0.051	2.602LysThr: 2.602 ± 0.055	2.882LysVal: 2.882 ± 0.072	0.358LysTrp: 0.358 ± 0.025	1.125LysTyr: 1.125 ± 0.041	0.0LysXaa: 0.0 ± 0.0
Leu	8.587LeuAla: 8.587 ± 0.114	1.183LeuCys: 1.183 ± 0.039	5.19LeuAsp: 5.19 ± 0.095	4.981LeuGlu: 4.981 ± 0.101	4.036LeuPhe: 4.036 ± 0.095	6.634LeuGly: 6.634 ± 0.103	1.966LeuHis: 1.966 ± 0.047	7.024LeuIle: 7.024 ± 0.133	3.992LeuLys: 3.992 ± 0.081	10.011LeuLeu: 10.011 ± 0.15	2.463LeuMet: 2.463 ± 0.05	3.409LeuAsn: 3.409 ± 0.063	4.579LeuPro: 4.579 ± 0.081	3.013LeuGln: 3.013 ± 0.062	4.433LeuArg: 4.433 ± 0.083	6.359LeuSer: 6.359 ± 0.103	5.981LeuThr: 5.981 ± 0.098	7.207LeuVal: 7.207 ± 0.113	0.841LeuTrp: 0.841 ± 0.035	2.599LeuTyr: 2.599 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	2.121MetAla: 2.121 ± 0.06	0.183MetCys: 0.183 ± 0.015	1.441MetAsp: 1.441 ± 0.043	1.497MetGlu: 1.497 ± 0.045	0.664MetPhe: 0.664 ± 0.031	1.816MetGly: 1.816 ± 0.052	0.491MetHis: 0.491 ± 0.023	2.135MetIle: 2.135 ± 0.051	1.539MetLys: 1.539 ± 0.046	2.266MetLeu: 2.266 ± 0.056	0.841MetMet: 0.841 ± 0.044	1.122MetAsn: 1.122 ± 0.037	1.105MetPro: 1.105 ± 0.04	0.922MetGln: 0.922 ± 0.034	1.2MetArg: 1.2 ± 0.041	1.269MetSer: 1.269 ± 0.038	1.886MetThr: 1.886 ± 0.05	2.148MetVal: 2.148 ± 0.054	0.144MetTrp: 0.144 ± 0.016	0.553MetTyr: 0.553 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	2.803AsnAla: 2.803 ± 0.08	0.511AsnCys: 0.511 ± 0.035	1.708AsnAsp: 1.708 ± 0.06	1.882AsnGlu: 1.882 ± 0.047	1.194AsnPhe: 1.194 ± 0.056	3.082AsnGly: 3.082 ± 0.08	0.665AsnHis: 0.665 ± 0.03	2.12AsnIle: 2.12 ± 0.064	1.096AsnLys: 1.096 ± 0.039	3.545AsnLeu: 3.545 ± 0.083	0.637AsnMet: 0.637 ± 0.027	1.488AsnAsn: 1.488 ± 0.07	2.146AsnPro: 2.146 ± 0.053	1.408AsnGln: 1.408 ± 0.051	2.126AsnArg: 2.126 ± 0.058	1.876AsnSer: 1.876 ± 0.06	2.371AsnThr: 2.371 ± 0.086	2.331AsnVal: 2.331 ± 0.073	0.408AsnTrp: 0.408 ± 0.022	1.1AsnTyr: 1.1 ± 0.06	0.0AsnXaa: 0.0 ± 0.0
Pro	4.545ProAla: 4.545 ± 0.081	0.571ProCys: 0.571 ± 0.027	3.2ProAsp: 3.2 ± 0.061	3.387ProGlu: 3.387 ± 0.072	1.892ProPhe: 1.892 ± 0.049	3.976ProGly: 3.976 ± 0.079	0.859ProHis: 0.859 ± 0.035	2.869ProIle: 2.869 ± 0.057	1.424ProLys: 1.424 ± 0.041	4.572ProLeu: 4.572 ± 0.088	1.011ProMet: 1.011 ± 0.036	1.337ProAsn: 1.337 ± 0.046	2.005ProPro: 2.005 ± 0.057	1.276ProGln: 1.276 ± 0.041	1.809ProArg: 1.809 ± 0.053	2.903ProSer: 2.903 ± 0.066	3.462ProThr: 3.462 ± 0.144	4.746ProVal: 4.746 ± 0.08	0.435ProTrp: 0.435 ± 0.023	1.364ProTyr: 1.364 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	2.599GlnAla: 2.599 ± 0.061	0.325GlnCys: 0.325 ± 0.022	1.576GlnAsp: 1.576 ± 0.045	1.974GlnGlu: 1.974 ± 0.052	1.441GlnPhe: 1.441 ± 0.055	2.425GlnGly: 2.425 ± 0.055	0.708GlnHis: 0.708 ± 0.03	2.627GlnIle: 2.627 ± 0.057	1.945GlnLys: 1.945 ± 0.06	2.258GlnLeu: 2.258 ± 0.051	1.139GlnMet: 1.139 ± 0.034	1.541GlnAsn: 1.541 ± 0.057	1.248GlnPro: 1.248 ± 0.038	1.57GlnGln: 1.57 ± 0.05	1.799GlnArg: 1.799 ± 0.047	1.939GlnSer: 1.939 ± 0.048	2.159GlnThr: 2.159 ± 0.056	3.069GlnVal: 3.069 ± 0.069	0.353GlnTrp: 0.353 ± 0.022	1.247GlnTyr: 1.247 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	4.281ArgAla: 4.281 ± 0.085	0.807ArgCys: 0.807 ± 0.037	3.26ArgAsp: 3.26 ± 0.065	4.063ArgGlu: 4.063 ± 0.075	2.385ArgPhe: 2.385 ± 0.06	3.827ArgGly: 3.827 ± 0.081	1.134ArgHis: 1.134 ± 0.04	3.94ArgIle: 3.94 ± 0.066	2.366ArgLys: 2.366 ± 0.065	4.874ArgLeu: 4.874 ± 0.086	1.666ArgMet: 1.666 ± 0.051	1.798ArgAsn: 1.798 ± 0.05	2.182ArgPro: 2.182 ± 0.054	1.902ArgGln: 1.902 ± 0.043	3.492ArgArg: 3.492 ± 0.084	3.397ArgSer: 3.397 ± 0.065	3.282ArgThr: 3.282 ± 0.068	4.183ArgVal: 4.183 ± 0.079	0.631ArgTrp: 0.631 ± 0.035	2.165ArgTyr: 2.165 ± 0.056	0.0ArgXaa: 0.0 ± 0.0
Ser	5.08SerAla: 5.08 ± 0.082	0.682SerCys: 0.682 ± 0.028	3.227SerAsp: 3.227 ± 0.058	3.174SerGlu: 3.174 ± 0.071	2.386SerPhe: 2.386 ± 0.064	5.924SerGly: 5.924 ± 0.111	1.134SerHis: 1.134 ± 0.04	4.081SerIle: 4.081 ± 0.083	1.786SerLys: 1.786 ± 0.047	5.892SerLeu: 5.892 ± 0.103	1.507SerMet: 1.507 ± 0.043	1.913SerAsn: 1.913 ± 0.074	3.193SerPro: 3.193 ± 0.07	1.803SerGln: 1.803 ± 0.047	3.196SerArg: 3.196 ± 0.062	4.396SerSer: 4.396 ± 0.09	4.44SerThr: 4.44 ± 0.115	4.678SerVal: 4.678 ± 0.078	0.682SerTrp: 0.682 ± 0.037	1.592SerTyr: 1.592 ± 0.047	0.0SerXaa: 0.0 ± 0.0
Thr	5.964ThrAla: 5.964 ± 0.123	0.784ThrCys: 0.784 ± 0.034	3.572ThrAsp: 3.572 ± 0.071	3.328ThrGlu: 3.328 ± 0.078	2.485ThrPhe: 2.485 ± 0.084	6.438ThrGly: 6.438 ± 0.124	1.206ThrHis: 1.206 ± 0.041	4.985ThrIle: 4.985 ± 0.096	1.863ThrLys: 1.863 ± 0.053	6.14ThrLeu: 6.14 ± 0.095	1.539ThrMet: 1.539 ± 0.04	2.002ThrAsn: 2.002 ± 0.102	4.184ThrPro: 4.184 ± 0.106	1.703ThrGln: 1.703 ± 0.058	3.063ThrArg: 3.063 ± 0.068	4.256ThrSer: 4.256 ± 0.107	5.491ThrThr: 5.491 ± 0.227	6.123ThrVal: 6.123 ± 0.167	0.731ThrTrp: 0.731 ± 0.037	2.046ThrTyr: 2.046 ± 0.083	0.0ThrXaa: 0.0 ± 0.0
Val	6.394ValAla: 6.394 ± 0.122	0.982ValCys: 0.982 ± 0.038	4.661ValAsp: 4.661 ± 0.088	4.156ValGlu: 4.156 ± 0.089	2.699ValPhe: 2.699 ± 0.059	5.051ValGly: 5.051 ± 0.094	1.66ValHis: 1.66 ± 0.047	5.717ValIle: 5.717 ± 0.092	3.082ValLys: 3.082 ± 0.066	7.574ValLeu: 7.574 ± 0.103	2.074ValMet: 2.074 ± 0.056	2.797ValAsn: 2.797 ± 0.082	4.176ValPro: 4.176 ± 0.081	2.732ValGln: 2.732 ± 0.065	4.299ValArg: 4.299 ± 0.084	4.702ValSer: 4.702 ± 0.088	5.839ValThr: 5.839 ± 0.132	6.1ValVal: 6.1 ± 0.105	0.654ValTrp: 0.654 ± 0.029	2.227ValTyr: 2.227 ± 0.067	0.0ValXaa: 0.0 ± 0.0
Trp	0.716TrpAla: 0.716 ± 0.032	0.145TrpCys: 0.145 ± 0.014	0.538TrpAsp: 0.538 ± 0.024	0.628TrpGlu: 0.628 ± 0.032	0.429TrpPhe: 0.429 ± 0.023	0.797TrpGly: 0.797 ± 0.043	0.209TrpHis: 0.209 ± 0.022	0.755TrpIle: 0.755 ± 0.032	0.516TrpLys: 0.516 ± 0.022	0.984TrpLeu: 0.984 ± 0.038	0.284TrpMet: 0.284 ± 0.019	0.531TrpAsn: 0.531 ± 0.027	0.301TrpPro: 0.301 ± 0.021	0.455TrpGln: 0.455 ± 0.022	0.562TrpArg: 0.562 ± 0.034	0.692TrpSer: 0.692 ± 0.042	0.627TrpThr: 0.627 ± 0.033	0.718TrpVal: 0.718 ± 0.032	0.165TrpTrp: 0.165 ± 0.015	0.384TrpTyr: 0.384 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.368TyrAla: 2.368 ± 0.061	0.406TyrCys: 0.406 ± 0.022	1.623TyrAsp: 1.623 ± 0.044	1.453TyrGlu: 1.453 ± 0.053	1.038TyrPhe: 1.038 ± 0.037	2.347TyrGly: 2.347 ± 0.053	0.787TyrHis: 0.787 ± 0.036	1.727TyrIle: 1.727 ± 0.051	0.796TyrLys: 0.796 ± 0.034	3.313TyrLeu: 3.313 ± 0.078	0.548TyrMet: 0.548 ± 0.025	1.139TyrAsn: 1.139 ± 0.045	1.562TyrPro: 1.562 ± 0.045	1.286TyrGln: 1.286 ± 0.048	2.168TyrArg: 2.168 ± 0.058	1.854TyrSer: 1.854 ± 0.056	2.166TyrThr: 2.166 ± 0.092	1.854TyrVal: 1.854 ± 0.062	0.378TyrTrp: 0.378 ± 0.025	1.06TyrTyr: 1.06 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2655 proteins (818093 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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