Microbulbifer sp. ZGT114

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Microbulbiferaceae; Microbulbifer; unclassified Microbulbifer

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3054 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0X3UCA4|A0A0X3UCA4_9GAMM PDZ domain-containing protein OS=Microbulbifer sp. ZGT114 OX=1685377 GN=AVO43_01180 PE=4 SV=1
MM1 pKa = 7.15KK2 pKa = 10.23HH3 pKa = 6.03VNSKK7 pKa = 10.54LLLSAAIAALLAGCDD22 pKa = 3.19SGGINIEE29 pKa = 4.32PEE31 pKa = 4.29TIDD34 pKa = 3.54NSVDD38 pKa = 3.18NSVVGGGNSEE48 pKa = 4.87DD49 pKa = 3.97NPCASYY55 pKa = 10.7EE56 pKa = 4.03KK57 pKa = 10.41SGQVQQGSFDD67 pKa = 3.9GTNCTYY73 pKa = 10.81AASFVDD79 pKa = 3.69SGNALTQDD87 pKa = 3.82LFIPALEE94 pKa = 4.05NDD96 pKa = 3.75GVHH99 pKa = 6.23IFEE102 pKa = 5.18GSLFVGNNYY111 pKa = 10.6DD112 pKa = 5.03DD113 pKa = 5.07DD114 pKa = 4.16ATMAAAGIAEE124 pKa = 4.7GGDD127 pKa = 3.7GAKK130 pKa = 9.15LTIEE134 pKa = 4.49AGSTIAFPNNTKK146 pKa = 10.57FIVINRR152 pKa = 11.84GSQLFAVGTPDD163 pKa = 3.97APITFTSTSDD173 pKa = 3.79AIDD176 pKa = 3.4NAVAFDD182 pKa = 5.55AVQQWGGMVINGFGITNKK200 pKa = 9.96CSYY203 pKa = 10.28DD204 pKa = 3.55ANLQTSEE211 pKa = 4.17CHH213 pKa = 6.2ILAEE217 pKa = 4.44GAAGKK222 pKa = 9.62DD223 pKa = 3.22QSNYY227 pKa = 10.82GGDD230 pKa = 3.58NNADD234 pKa = 3.12NSGQLEE240 pKa = 4.42YY241 pKa = 11.33VRR243 pKa = 11.84VKK245 pKa = 9.89HH246 pKa = 6.06TGAQVASGDD255 pKa = 4.01EE256 pKa = 4.15LNGITFSSVGSGTIVKK272 pKa = 9.66NLQVYY277 pKa = 7.67STYY280 pKa = 11.19DD281 pKa = 3.1DD282 pKa = 4.67GIEE285 pKa = 4.02MFGGAVSFEE294 pKa = 4.78NYY296 pKa = 9.33LAMYY300 pKa = 10.03VRR302 pKa = 11.84DD303 pKa = 4.14DD304 pKa = 5.1SIDD307 pKa = 3.38IDD309 pKa = 4.09EE310 pKa = 4.88GWAGSITNALVIQSEE325 pKa = 4.59TDD327 pKa = 3.22GNHH330 pKa = 6.72CIEE333 pKa = 4.25SDD335 pKa = 4.26GIGSYY340 pKa = 10.63DD341 pKa = 3.89DD342 pKa = 4.21KK343 pKa = 11.53DD344 pKa = 3.34AAFIDD349 pKa = 4.59GVISQGINSRR359 pKa = 11.84PVIDD363 pKa = 5.01GLTCIISANGADD375 pKa = 4.2TATHH379 pKa = 6.97DD380 pKa = 4.54PGAGWRR386 pKa = 11.84FRR388 pKa = 11.84EE389 pKa = 4.63AIFPKK394 pKa = 8.92ITNSMVVGSFSADD407 pKa = 3.1TTGVDD412 pKa = 3.35GSNYY416 pKa = 9.15CLRR419 pKa = 11.84VEE421 pKa = 4.44DD422 pKa = 4.89PEE424 pKa = 4.44TLVEE428 pKa = 4.25AEE430 pKa = 4.74VGTEE434 pKa = 3.83LQIEE438 pKa = 4.58SNIFACEE445 pKa = 3.87DD446 pKa = 3.43RR447 pKa = 11.84TKK449 pKa = 10.7GGPVGTQTLEE459 pKa = 3.49EE460 pKa = 3.99WAVANGNVFADD471 pKa = 3.27ITGAVDD477 pKa = 3.59PTAMANTEE485 pKa = 4.04LQILEE490 pKa = 4.25GVPSVFSIDD499 pKa = 3.47AASMQVNGAAIGISPIEE516 pKa = 3.75RR517 pKa = 11.84AYY519 pKa = 10.77FGALSQGDD527 pKa = 4.51LDD529 pKa = 3.86WTLGWTYY536 pKa = 11.21GLHH539 pKa = 6.04NGEE542 pKa = 4.94ALWFANN548 pKa = 3.92

Molecular weight: 57.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0X3U4Z2|A0A0X3U4Z2_9GAMM Gamma carbonic anhydrase family protein OS=Microbulbifer sp. ZGT114 OX=1685377 GN=AVO43_11040 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.16RR12 pKa = 11.84KK13 pKa = 9.08RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.27NGRR28 pKa = 11.84KK29 pKa = 8.87ILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.95GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3054

0

3054

1034147

38

3367

338.6

37.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.498 ± 0.051

0.991 ± 0.017

5.733 ± 0.038

6.894 ± 0.043

3.754 ± 0.027

8.048 ± 0.037

2.13 ± 0.024

4.846 ± 0.032

3.467 ± 0.037

10.794 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.021

3.088 ± 0.029

4.72 ± 0.029

4.175 ± 0.032

6.697 ± 0.047

5.824 ± 0.03

4.807 ± 0.037

7.087 ± 0.043

1.404 ± 0.019

2.741 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski