Kushneria marisflavi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Kushneria

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3205 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A240UKN6|A0A240UKN6_9GAMM Dehydrogenase OS=Kushneria marisflavi OX=157779 GN=B9H00_02255 PE=4 SV=1
MM1 pKa = 8.1NMMMSSRR8 pKa = 11.84VDD10 pKa = 3.43ADD12 pKa = 3.33QTPCMAMSEE21 pKa = 4.69PPVQPPDD28 pKa = 4.23PEE30 pKa = 4.11PHH32 pKa = 6.56SPPEE36 pKa = 4.08QPPQPQEE43 pKa = 3.92PPIEE47 pKa = 4.36EE48 pKa = 4.54PPMEE52 pKa = 4.9EE53 pKa = 5.31PPLQDD58 pKa = 4.48PPPSQPPPEE67 pKa = 4.42VPPEE71 pKa = 4.03QTPHH75 pKa = 5.29YY76 pKa = 10.16QSS78 pKa = 3.46

Molecular weight: 8.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A240UJL9|A0A240UJL9_9GAMM NAD(P)H:quinone oxidoreductase type IV OS=Kushneria marisflavi OX=157779 GN=B9H00_00315 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.32NGRR28 pKa = 11.84QVISRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.97GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3205

0

3205

1071555

26

2911

334.3

36.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.446 ± 0.046

0.964 ± 0.014

5.9 ± 0.035

6.046 ± 0.044

3.506 ± 0.03

8.072 ± 0.042

2.655 ± 0.025

5.178 ± 0.033

2.556 ± 0.035

11.019 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.844 ± 0.021

2.8 ± 0.03

4.691 ± 0.029

4.068 ± 0.03

7.24 ± 0.047

5.944 ± 0.032

5.27 ± 0.028

6.964 ± 0.034

1.481 ± 0.022

2.354 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski