CrAssphage sp.

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae; crAss-like viruses

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A345BP28|A0A345BP28_9CAUD Putative tail tubular protein OS=CrAssphage sp. OX=2212563 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.25LYY4 pKa = 10.53KK5 pKa = 10.66DD6 pKa = 3.73NLGKK10 pKa = 10.53VAITVEE16 pKa = 3.64QGYY19 pKa = 9.4WDD21 pKa = 3.77EE22 pKa = 4.54NKK24 pKa = 10.7DD25 pKa = 3.66YY26 pKa = 11.56DD27 pKa = 3.87KK28 pKa = 10.87LTVVEE33 pKa = 4.3RR34 pKa = 11.84EE35 pKa = 4.69GIFGTYY41 pKa = 9.35ISRR44 pKa = 11.84KK45 pKa = 7.74PVPAGTEE52 pKa = 3.95LSDD55 pKa = 3.18RR56 pKa = 11.84TYY58 pKa = 10.2WIPFSSLKK66 pKa = 10.52EE67 pKa = 3.95EE68 pKa = 3.87IVINYY73 pKa = 9.97NEE75 pKa = 4.91FISHH79 pKa = 6.7YY80 pKa = 10.93GPMLDD85 pKa = 5.08DD86 pKa = 3.47QAEE89 pKa = 4.31VLNNVQLLINEE100 pKa = 5.03IYY102 pKa = 10.72NKK104 pKa = 9.96LPSGIIFEE112 pKa = 4.34GDD114 pKa = 2.92KK115 pKa = 10.72TFIRR119 pKa = 11.84PGHH122 pKa = 6.12PEE124 pKa = 4.42EE125 pKa = 4.09ITLTGSTVNEE135 pKa = 4.22DD136 pKa = 3.21PATFKK141 pKa = 10.83LYY143 pKa = 10.76KK144 pKa = 9.87NSKK147 pKa = 9.35LVHH150 pKa = 5.35TVTAKK155 pKa = 10.48SFTYY159 pKa = 10.21KK160 pKa = 10.72AIADD164 pKa = 3.98DD165 pKa = 3.64EE166 pKa = 4.55CAYY169 pKa = 10.17KK170 pKa = 10.75LIVEE174 pKa = 4.2QSGYY178 pKa = 10.61RR179 pKa = 11.84YY180 pKa = 9.31VAGWNITLRR189 pKa = 11.84YY190 pKa = 8.34PIYY193 pKa = 10.17AGSGATYY200 pKa = 9.97EE201 pKa = 5.06DD202 pKa = 4.06VVSDD206 pKa = 3.68YY207 pKa = 11.53NIIKK211 pKa = 9.97IDD213 pKa = 3.69TNPEE217 pKa = 3.12GLYY220 pKa = 10.11IINVINDD227 pKa = 3.03GDD229 pKa = 4.35YY230 pKa = 11.14IFFVAPKK237 pKa = 9.83NVIIHH242 pKa = 5.95SIKK245 pKa = 10.89LNGLDD250 pKa = 3.56VPLKK254 pKa = 8.6QTEE257 pKa = 4.22EE258 pKa = 4.31VEE260 pKa = 4.8LEE262 pKa = 4.01TEE264 pKa = 4.24YY265 pKa = 10.48IIYY268 pKa = 10.59QSDD271 pKa = 3.19NTYY274 pKa = 10.89EE275 pKa = 4.22SNNYY279 pKa = 8.85PIYY282 pKa = 9.56INSYY286 pKa = 8.8EE287 pKa = 4.09GLNGGDD293 pKa = 3.49FVKK296 pKa = 10.59ILDD299 pKa = 3.75VLQDD303 pKa = 3.52KK304 pKa = 8.12VTSEE308 pKa = 4.85DD309 pKa = 2.65ITRR312 pKa = 11.84ITNEE316 pKa = 3.68NLEE319 pKa = 4.29IKK321 pKa = 8.9QTLQQVKK328 pKa = 10.29EE329 pKa = 4.25EE330 pKa = 4.13NNEE333 pKa = 3.75VNNNIDD339 pKa = 3.21AALSVVEE346 pKa = 4.55TYY348 pKa = 8.69KK349 pKa = 10.69TEE351 pKa = 4.12INKK354 pKa = 9.98SLAIIEE360 pKa = 4.7NISNEE365 pKa = 4.0LNDD368 pKa = 3.02ITEE371 pKa = 5.01NINTSNVDD379 pKa = 3.83LNVLKK384 pKa = 10.37TEE386 pKa = 4.21VTNMASSVNSLNKK399 pKa = 10.21SLEE402 pKa = 4.03EE403 pKa = 4.33LGTNVYY409 pKa = 10.43SAIDD413 pKa = 3.53AVQEE417 pKa = 3.96NVDD420 pKa = 4.08RR421 pKa = 11.84IGEE424 pKa = 4.13GRR426 pKa = 11.84VDD428 pKa = 4.72IINEE432 pKa = 3.83DD433 pKa = 3.52GFRR436 pKa = 11.84NNNVIYY442 pKa = 10.2LKK444 pKa = 10.65PNWTKK449 pKa = 10.34VSVYY453 pKa = 10.34GNEE456 pKa = 3.97PEE458 pKa = 4.08IVPVLTQNMINRR470 pKa = 11.84PYY472 pKa = 7.32TTYY475 pKa = 10.95VIDD478 pKa = 3.47YY479 pKa = 9.41NYY481 pKa = 10.92YY482 pKa = 9.52IFDD485 pKa = 4.49TIEE488 pKa = 4.13LPIGTTLKK496 pKa = 10.36FRR498 pKa = 11.84AGALLFVEE506 pKa = 4.53NGSMYY511 pKa = 10.57SQYY514 pKa = 11.51NCIIDD519 pKa = 4.1DD520 pKa = 4.27CGNNNIIQGEE530 pKa = 4.37TLAMQIVFSNVYY542 pKa = 10.46LDD544 pKa = 3.44WFYY547 pKa = 11.81LFLDD551 pKa = 3.25IGGSYY556 pKa = 10.39VEE558 pKa = 4.48NLNGILDD565 pKa = 3.93YY566 pKa = 11.11AFSIARR572 pKa = 11.84GPIRR576 pKa = 11.84FRR578 pKa = 11.84QVDD581 pKa = 3.73RR582 pKa = 11.84LVIDD586 pKa = 4.29GFVNACVGTIDD597 pKa = 5.54AEE599 pKa = 4.52SKK601 pKa = 10.71SININNCEE609 pKa = 3.93NATITIQNSNSVIIEE624 pKa = 4.01KK625 pKa = 10.39ADD627 pKa = 4.01NISDD631 pKa = 3.61VSEE634 pKa = 3.94ICIKK638 pKa = 10.9NSIIDD643 pKa = 3.77VINIYY648 pKa = 9.89SPCTLSLRR656 pKa = 11.84IDD658 pKa = 3.35NCNINEE664 pKa = 4.02IQAVCDD670 pKa = 4.25DD671 pKa = 3.93GVLTIEE677 pKa = 5.16NSIIQNNIVSSFYY690 pKa = 11.19TNIIRR695 pKa = 11.84NNVMLHH701 pKa = 4.93TAYY704 pKa = 9.8ISFVQSGIYY713 pKa = 9.72KK714 pKa = 9.96ILEE717 pKa = 4.15APKK720 pKa = 9.57TAIIDD725 pKa = 3.55NNIIGEE731 pKa = 4.34VILDD735 pKa = 3.65EE736 pKa = 6.15FEE738 pKa = 4.58FLDD741 pKa = 5.06DD742 pKa = 3.8FTTAIPIIFEE752 pKa = 4.04FYY754 pKa = 10.76GYY756 pKa = 9.72IPHH759 pKa = 6.69YY760 pKa = 9.87NFSFIINNTNVDD772 pKa = 4.38LNIQNDD778 pKa = 3.44NKK780 pKa = 10.47FAGIISNSNVYY791 pKa = 10.44LHH793 pKa = 6.54LHH795 pKa = 5.66SLNTALEE802 pKa = 4.44TYY804 pKa = 10.52EE805 pKa = 4.7NVLLYY810 pKa = 8.88EE811 pKa = 4.56TFLHH815 pKa = 7.22LYY817 pKa = 8.89NCVVEE822 pKa = 4.33NSPNSEE828 pKa = 3.9VSSSDD833 pKa = 3.37DD834 pKa = 3.34YY835 pKa = 11.78CIISSTLTFDD845 pKa = 2.92NCYY848 pKa = 10.87ANNLGTIVGYY858 pKa = 5.9EE859 pKa = 3.69TSYY862 pKa = 11.05IRR864 pKa = 11.84TNNSPISVNEE874 pKa = 3.8DD875 pKa = 2.94EE876 pKa = 5.29LLYY879 pKa = 11.08GHH881 pKa = 7.24TYY883 pKa = 10.34DD884 pKa = 3.53ISRR887 pKa = 11.84GTVINTEE894 pKa = 3.92NKK896 pKa = 10.19LGISRR901 pKa = 11.84GRR903 pKa = 11.84NKK905 pKa = 10.45EE906 pKa = 4.05PLWLEE911 pKa = 3.92

Molecular weight:
103.08 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A345BNZ9|A0A345BNZ9_9CAUD Uncharacterized protein OS=CrAssphage sp. OX=2212563 PE=4 SV=1
MM1 pKa = 7.52NIAIRR6 pKa = 11.84YY7 pKa = 7.09KK8 pKa = 10.68FKK10 pKa = 10.75FNKK13 pKa = 8.15ITIFTICINLLINISISIYY32 pKa = 9.28YY33 pKa = 8.44LHH35 pKa = 7.25KK36 pKa = 10.54SIYY39 pKa = 7.95SKK41 pKa = 10.92KK42 pKa = 10.03IIRR45 pKa = 11.84STNMMTKK52 pKa = 9.91HH53 pKa = 6.03YY54 pKa = 10.04IASRR58 pKa = 11.84YY59 pKa = 9.58NKK61 pKa = 9.69FIFYY65 pKa = 10.39IKK67 pKa = 10.02ILYY70 pKa = 9.21KK71 pKa = 10.58CIISFSTFNKK81 pKa = 9.43NRR83 pKa = 11.84NNTYY87 pKa = 8.41TSIFIVSFSRR97 pKa = 11.84KK98 pKa = 8.17NNSRR102 pKa = 11.84KK103 pKa = 9.25FFSKK107 pKa = 10.5NSSNKK112 pKa = 9.39TIIRR116 pKa = 11.84SNIYY120 pKa = 9.22SRR122 pKa = 11.84CNISYY127 pKa = 10.65ILFCNLINRR136 pKa = 11.84CWKK139 pKa = 10.17INIKK143 pKa = 10.43FSVLTFYY150 pKa = 10.9IKK152 pKa = 9.86MYY154 pKa = 10.83HH155 pKa = 6.58NINLINNKK163 pKa = 9.77YY164 pKa = 9.97NILFIFIIILYY175 pKa = 9.69NILYY179 pKa = 9.61YY180 pKa = 10.1II181 pKa = 4.5

Molecular weight:
22.04 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

27590

81

4253

344.9

39.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.625 ± 0.353

1.225 ± 0.185

6.292 ± 0.184

6.524 ± 0.296

4.215 ± 0.147

5.259 ± 0.26

1.591 ± 0.103

8.499 ± 0.418

8.307 ± 0.306

7.506 ± 0.176

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.222 ± 0.145

8.311 ± 0.26

2.588 ± 0.129

3.226 ± 0.241

3.987 ± 0.14

6.557 ± 0.198

6.444 ± 0.23

5.676 ± 0.205

0.819 ± 0.086

5.125 ± 0.251

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski