Rothia phage Spartoi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5K7NMS2|A0A5K7NMS2_9CAUD Uncharacterized protein OS=Rothia phage Spartoi OX=2483661 GN=29 PE=4 SV=1
MM1 pKa = 7.72EE2 pKa = 5.1LTLQQLQAKK11 pKa = 8.93CIYY14 pKa = 10.02LAEE17 pKa = 4.39EE18 pKa = 3.81NAQLRR23 pKa = 11.84DD24 pKa = 3.53ALLDD28 pKa = 3.76AKK30 pKa = 10.67LQLGVIALDD39 pKa = 3.72NQEE42 pKa = 4.11PQANSGEE49 pKa = 4.44TSEE52 pKa = 4.46EE53 pKa = 4.12THH55 pKa = 6.77NDD57 pKa = 2.97

Molecular weight: 6.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5K7NMH8|A0A5K7NMH8_9CAUD Uncharacterized protein OS=Rothia phage Spartoi OX=2483661 GN=9 PE=4 SV=1
MM1 pKa = 7.81PSVQSLRR8 pKa = 11.84GKK10 pKa = 10.06AVVRR14 pKa = 11.84HH15 pKa = 5.89GPPPNLRR22 pKa = 11.84GGGRR26 pKa = 11.84NSGGGNDD33 pKa = 4.39APLGVPPIRR42 pKa = 11.84GAGSFVLSPAPLAIPPADD60 pKa = 3.36RR61 pKa = 11.84SKK63 pKa = 11.07EE64 pKa = 3.89HH65 pKa = 5.95WQHH68 pKa = 7.07LEE70 pKa = 3.78QEE72 pKa = 4.41PQSGKK77 pKa = 9.81PP78 pKa = 3.24

Molecular weight: 8.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11117

25

1174

198.5

21.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.127 ± 0.556

0.774 ± 0.138

4.821 ± 0.245

7.097 ± 0.409

3.292 ± 0.237

8.15 ± 0.42

1.781 ± 0.157

6.099 ± 0.357

5.991 ± 0.338

7.781 ± 0.339

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.258 ± 0.136

3.868 ± 0.202

5.118 ± 0.236

3.823 ± 0.225

5.604 ± 0.426

5.784 ± 0.297

6.009 ± 0.312

6.629 ± 0.313

1.727 ± 0.15

2.267 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski