Streptomyces natalensis ATCC 27448

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces natalensis

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6559 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D7CIG6|A0A0D7CIG6_9ACTN Uncharacterized protein OS=Streptomyces natalensis ATCC 27448 OX=1240678 GN=SNA_22740 PE=4 SV=1
MM1 pKa = 7.77PEE3 pKa = 3.74VTSAYY8 pKa = 10.28QPGTPCWVDD17 pKa = 3.59LAAPDD22 pKa = 3.44QQAAIDD28 pKa = 4.57FYY30 pKa = 11.62SSVFGWTGEE39 pKa = 3.86IGPPEE44 pKa = 4.03TGGYY48 pKa = 9.06AVCEE52 pKa = 4.16LNGKK56 pKa = 8.13PVAGIMAAMTMDD68 pKa = 4.51GQPPPPTVWTTYY80 pKa = 10.29LAAADD85 pKa = 4.18ADD87 pKa = 3.77ATAQAVSSAGGTIQMPVVDD106 pKa = 4.87MMTLGRR112 pKa = 11.84MCIAADD118 pKa = 3.33PAGAVFGIWQAVDD131 pKa = 3.73FPGAGIVNEE140 pKa = 4.18HH141 pKa = 6.83GALIWNEE148 pKa = 3.91LNTTDD153 pKa = 4.52RR154 pKa = 11.84SAAAAFYY161 pKa = 9.74QASFGIEE168 pKa = 3.88SAAMEE173 pKa = 4.05MAGAEE178 pKa = 4.1NYY180 pKa = 8.14FALKK184 pKa = 10.67VGDD187 pKa = 3.86RR188 pKa = 11.84PVGGMQPMPEE198 pKa = 4.22QMPPDD203 pKa = 4.35TPSHH207 pKa = 5.87WLTYY211 pKa = 9.78FSVPDD216 pKa = 3.42TDD218 pKa = 3.53ATVEE222 pKa = 4.19KK223 pKa = 9.79VTAAGGAALQEE234 pKa = 4.95PIDD237 pKa = 3.97MVAGRR242 pKa = 11.84MAMVTDD248 pKa = 3.88PQGATFAVISPKK260 pKa = 11.12SMDD263 pKa = 3.4LGG265 pKa = 3.93

Molecular weight: 27.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D7CUZ1|A0A0D7CUZ1_9ACTN ATP-binding protein OS=Streptomyces natalensis ATCC 27448 OX=1240678 GN=SNA_00315 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.36GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6559

0

6559

2045269

29

9507

311.8

33.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.797 ± 0.05

0.852 ± 0.009

5.917 ± 0.023

5.57 ± 0.029

2.717 ± 0.018

9.407 ± 0.032

2.469 ± 0.015

3.328 ± 0.022

2.299 ± 0.027

10.359 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.834 ± 0.013

1.765 ± 0.017

6.163 ± 0.027

2.945 ± 0.02

8.046 ± 0.034

4.908 ± 0.023

6.041 ± 0.022

8.01 ± 0.029

1.504 ± 0.013

2.067 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski