Rufibacter radiotolerans

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Rufibacter

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3455 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H4VIE8|A0A0H4VIE8_9BACT NADH-quinone oxidoreductase subunit K OS=Rufibacter radiotolerans OX=1379910 GN=nuoK PE=3 SV=1
MM1 pKa = 7.23FANEE5 pKa = 4.18LRR7 pKa = 11.84LGNIVSEE14 pKa = 4.22AGTYY18 pKa = 9.5VVIDD22 pKa = 4.48AGRR25 pKa = 11.84MMAILSGEE33 pKa = 3.94AALYY37 pKa = 10.55DD38 pKa = 5.09PIPLTDD44 pKa = 3.32EE45 pKa = 4.07WLSGANYY52 pKa = 10.54DD53 pKa = 3.87EE54 pKa = 5.78LEE56 pKa = 4.45DD57 pKa = 3.86EE58 pKa = 5.1FMFPEE63 pKa = 4.89LSQWALDD70 pKa = 3.76PKK72 pKa = 10.51YY73 pKa = 10.88KK74 pKa = 10.55EE75 pKa = 3.71IVFNGGYY82 pKa = 9.37IATSAPVAYY91 pKa = 9.96VHH93 pKa = 6.1QLQNFFFVMTGQEE106 pKa = 4.04MEE108 pKa = 4.78LPCHH112 pKa = 6.0LQLL115 pKa = 5.62

Molecular weight: 12.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H4W668|A0A0H4W668_9BACT Phosphate transport system permease protein OS=Rufibacter radiotolerans OX=1379910 GN=TH63_10205 PE=3 SV=1
MM1 pKa = 6.3QQEE4 pKa = 4.4VKK6 pKa = 10.72NVFGRR11 pKa = 11.84TFYY14 pKa = 10.83RR15 pKa = 11.84IRR17 pKa = 11.84YY18 pKa = 6.49NAQSNMVEE26 pKa = 4.37AEE28 pKa = 3.72WHH30 pKa = 5.75GAATQQDD37 pKa = 3.71LRR39 pKa = 11.84KK40 pKa = 9.66AVVAGLEE47 pKa = 3.83LHH49 pKa = 6.45EE50 pKa = 4.34RR51 pKa = 11.84TRR53 pKa = 11.84CAFRR57 pKa = 11.84LNYY60 pKa = 8.45NTGSSGPWSDD70 pKa = 3.26SVTWLEE76 pKa = 4.54KK77 pKa = 9.81EE78 pKa = 3.81WLPRR82 pKa = 11.84FPRR85 pKa = 11.84RR86 pKa = 11.84ARR88 pKa = 11.84GNRR91 pKa = 11.84LSKK94 pKa = 10.43PSPKK98 pKa = 10.54

Molecular weight: 11.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3455

0

3455

1172869

54

4508

339.5

37.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.245 ± 0.045

0.714 ± 0.015

4.873 ± 0.027

6.142 ± 0.047

4.712 ± 0.032

7.19 ± 0.039

2.044 ± 0.024

5.726 ± 0.035

6.081 ± 0.039

10.325 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.324 ± 0.02

4.507 ± 0.042

4.41 ± 0.024

4.54 ± 0.035

4.511 ± 0.031

5.863 ± 0.034

5.896 ± 0.056

6.963 ± 0.035

1.205 ± 0.017

3.728 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski