Gordonia phage GMA4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K0N5V8|A0A0K0N5V8_9CAUD Uncharacterized protein OS=Gordonia phage GMA4 OX=1647471 GN=GMA4_49 PE=4 SV=1
MM1 pKa = 8.02CDD3 pKa = 3.16LSDD6 pKa = 4.31ARR8 pKa = 11.84RR9 pKa = 11.84DD10 pKa = 3.9AINDD14 pKa = 4.18HH15 pKa = 7.0IDD17 pKa = 3.59FDD19 pKa = 4.52PPDD22 pKa = 3.98TFDD25 pKa = 3.63YY26 pKa = 11.32APDD29 pKa = 3.32QTFPPFPDD37 pKa = 5.55DD38 pKa = 4.18YY39 pKa = 11.59EE40 pKa = 4.17QDD42 pKa = 3.54QPP44 pKa = 4.27

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K0N652|A0A0K0N652_9CAUD Uncharacterized protein OS=Gordonia phage GMA4 OX=1647471 GN=GMA4_40 PE=4 SV=1
MM1 pKa = 7.0TAQGRR6 pKa = 11.84ATMPGAVSAEE16 pKa = 3.59RR17 pKa = 11.84RR18 pKa = 11.84AAMAARR24 pKa = 11.84RR25 pKa = 11.84DD26 pKa = 3.63AARR29 pKa = 11.84RR30 pKa = 11.84AAWAEE35 pKa = 4.05RR36 pKa = 11.84IFEE39 pKa = 4.32TTAAAYY45 pKa = 7.24VHH47 pKa = 6.52RR48 pKa = 11.84HH49 pKa = 5.65LGTSIRR55 pKa = 11.84ALEE58 pKa = 4.17HH59 pKa = 6.29EE60 pKa = 4.52KK61 pKa = 10.38QHH63 pKa = 5.98LHH65 pKa = 6.16ALKK68 pKa = 10.61ADD70 pKa = 3.72VLEE73 pKa = 4.47TQAVVRR79 pKa = 11.84EE80 pKa = 4.25LAVSAA85 pKa = 4.59

Molecular weight: 9.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

13952

40

1760

205.2

22.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.844 ± 0.507

0.896 ± 0.159

6.795 ± 0.367

5.426 ± 0.364

2.602 ± 0.201

8.493 ± 0.444

2.071 ± 0.287

4.544 ± 0.156

3.319 ± 0.206

8.192 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.114 ± 0.137

2.58 ± 0.191

5.526 ± 0.288

3.727 ± 0.299

7.526 ± 0.522

5.196 ± 0.348

6.952 ± 0.381

7.397 ± 0.298

1.993 ± 0.152

1.806 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski