Candidatus Annandia adelgestsuga

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacteriaceae incertae sedis; ant, tsetse, mealybug, aphid, etc. endosymbionts; Candidatus Annandia

Average proteome isoelectric point is 8.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 285 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q9CLV2|A0A3Q9CLV2_9ENTR Methionine--tRNA ligase OS=Candidatus Annandia adelgestsuga OX=1302411 GN=metG PE=3 SV=1
MM1 pKa = 6.63TAKK4 pKa = 10.41KK5 pKa = 10.2IKK7 pKa = 9.93FGNDD11 pKa = 2.61ARR13 pKa = 11.84SKK15 pKa = 10.02ILKK18 pKa = 9.55GVNLLADD25 pKa = 3.83AVKK28 pKa = 9.58VTLGPKK34 pKa = 9.43GRR36 pKa = 11.84NAVIDD41 pKa = 3.92KK42 pKa = 11.07SFGAPIITKK51 pKa = 10.46DD52 pKa = 3.23GVTVARR58 pKa = 11.84EE59 pKa = 3.62IEE61 pKa = 4.11LDD63 pKa = 3.77DD64 pKa = 4.3KK65 pKa = 11.13FEE67 pKa = 4.52NMGAQMVKK75 pKa = 10.19EE76 pKa = 4.43VASKK80 pKa = 11.37ANDD83 pKa = 3.44AAGDD87 pKa = 3.92GTTTATLLAQSIVNEE102 pKa = 4.04GLKK105 pKa = 10.61AVAAGMNPMDD115 pKa = 5.27LKK117 pKa = 10.96RR118 pKa = 11.84GIDD121 pKa = 3.39KK122 pKa = 10.97AVILAVRR129 pKa = 11.84EE130 pKa = 3.99LKK132 pKa = 10.44IISVPCEE139 pKa = 3.71DD140 pKa = 4.41SKK142 pKa = 11.87SITQVGTISANADD155 pKa = 3.45EE156 pKa = 5.06NVGKK160 pKa = 10.5LISQAMSKK168 pKa = 10.08VGKK171 pKa = 9.73EE172 pKa = 3.81GVITVEE178 pKa = 4.33EE179 pKa = 4.62GTGLDD184 pKa = 3.95DD185 pKa = 4.36EE186 pKa = 5.38LDD188 pKa = 3.84VVEE191 pKa = 6.18GMQFDD196 pKa = 3.76RR197 pKa = 11.84GYY199 pKa = 10.76LSPYY203 pKa = 9.94FINKK207 pKa = 8.4QDD209 pKa = 3.55NGTVEE214 pKa = 4.42LDD216 pKa = 3.19NPYY219 pKa = 10.58ILLVEE224 pKa = 4.38KK225 pKa = 10.27KK226 pKa = 9.77ISNIRR231 pKa = 11.84EE232 pKa = 3.97LLSILEE238 pKa = 4.32NVAKK242 pKa = 10.55SNKK245 pKa = 8.83PMLIIAEE252 pKa = 4.68DD253 pKa = 3.63IEE255 pKa = 4.92GEE257 pKa = 4.08ALATLVVNNMRR268 pKa = 11.84GIVKK272 pKa = 9.77VAAVKK277 pKa = 10.73APGFGDD283 pKa = 3.21RR284 pKa = 11.84RR285 pKa = 11.84KK286 pKa = 11.2SMLQDD291 pKa = 3.1IAILTGGTVISEE303 pKa = 4.37EE304 pKa = 3.89IGIEE308 pKa = 3.87LEE310 pKa = 4.74KK311 pKa = 10.91INLEE315 pKa = 4.08NLGQAKK321 pKa = 9.64KK322 pKa = 11.06VIINKK327 pKa = 10.02DD328 pKa = 2.97NTTIIDD334 pKa = 4.67GIGKK338 pKa = 8.9EE339 pKa = 4.15KK340 pKa = 10.41KK341 pKa = 9.79IKK343 pKa = 10.59DD344 pKa = 3.48RR345 pKa = 11.84VKK347 pKa = 10.59QIHH350 pKa = 4.81QQIKK354 pKa = 9.5EE355 pKa = 4.06ATSDD359 pKa = 3.46YY360 pKa = 11.27DD361 pKa = 3.8KK362 pKa = 11.47EE363 pKa = 4.15KK364 pKa = 10.37LQEE367 pKa = 4.01RR368 pKa = 11.84VAKK371 pKa = 10.23LAGGVAVLKK380 pKa = 10.81VGAATEE386 pKa = 4.21VEE388 pKa = 4.37MKK390 pKa = 10.02EE391 pKa = 3.77KK392 pKa = 10.23KK393 pKa = 10.49ARR395 pKa = 11.84VEE397 pKa = 4.2DD398 pKa = 3.97ALHH401 pKa = 5.68ATRR404 pKa = 11.84AAVEE408 pKa = 4.18EE409 pKa = 4.64GVVAGGGVALIRR421 pKa = 11.84VASRR425 pKa = 11.84ISHH428 pKa = 6.46MIGQNEE434 pKa = 4.16DD435 pKa = 3.23QNVGIRR441 pKa = 11.84IAIRR445 pKa = 11.84AMEE448 pKa = 4.4APLRR452 pKa = 11.84QIIANAGEE460 pKa = 4.23EE461 pKa = 4.03PSVISNAIKK470 pKa = 10.41SGKK473 pKa = 9.57GNYY476 pKa = 9.11GYY478 pKa = 10.9NAATSEE484 pKa = 4.4YY485 pKa = 10.58GNMINFGILDD495 pKa = 3.74PTKK498 pKa = 8.82VTRR501 pKa = 11.84SALQYY506 pKa = 9.29ASSVAGLMITTEE518 pKa = 4.14CMITDD523 pKa = 3.6SSKK526 pKa = 11.2EE527 pKa = 4.05EE528 pKa = 4.1KK529 pKa = 10.51NDD531 pKa = 3.69TNNSIPSGGMGGMGGMMM548 pKa = 4.58

Molecular weight: 58.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S9J7J5|A0A3S9J7J5_9ENTR 3-isopropylmalate dehydratase large subunit OS=Candidatus Annandia adelgestsuga OX=1302411 GN=leuC PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.58RR3 pKa = 11.84TFQPSLIKK11 pKa = 10.32KK12 pKa = 9.09KK13 pKa = 9.63RR14 pKa = 11.84SHH16 pKa = 5.84GFRR19 pKa = 11.84YY20 pKa = 10.12RR21 pKa = 11.84MSTKK25 pKa = 9.94NGRR28 pKa = 11.84NILSKK33 pKa = 10.55RR34 pKa = 11.84RR35 pKa = 11.84NKK37 pKa = 9.76KK38 pKa = 10.16RR39 pKa = 11.84LFLTISSKK47 pKa = 11.21

Molecular weight: 5.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

285

0

285

90704

38

1336

318.3

36.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.728 ± 0.114

1.274 ± 0.048

3.291 ± 0.079

3.755 ± 0.108

5.439 ± 0.147

5.025 ± 0.134

1.428 ± 0.045

15.149 ± 0.194

14.082 ± 0.251

9.431 ± 0.136

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.011 ± 0.052

10.228 ± 0.187

2.581 ± 0.068

1.843 ± 0.051

2.687 ± 0.081

6.34 ± 0.109

3.57 ± 0.08

3.361 ± 0.099

0.766 ± 0.043

5.01 ± 0.108
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski