Microbacterium phage Elva

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dismasvirus; unclassified Dismasvirus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R4A1C1|A0A2R4A1C1_9CAUD ParB-like nuclease domain protein OS=Microbacterium phage Elva OX=2126929 GN=51 PE=4 SV=1
MM1 pKa = 7.71SDD3 pKa = 3.56FTAALDD9 pKa = 3.76AALPKK14 pKa = 8.84PTAGALPSSRR24 pKa = 11.84TFGMVTEE31 pKa = 4.44ALEE34 pKa = 4.53SKK36 pKa = 10.82DD37 pKa = 3.43VDD39 pKa = 3.87HH40 pKa = 7.62LSGEE44 pKa = 4.36LQHH47 pKa = 6.93SRR49 pKa = 11.84AHH51 pKa = 5.15YY52 pKa = 10.01QSLEE56 pKa = 3.84YY57 pKa = 10.15MLNNEE62 pKa = 4.4DD63 pKa = 5.15AIVATILGSFEE74 pKa = 4.2EE75 pKa = 4.84APEE78 pKa = 4.54LPTPTTVEE86 pKa = 3.67EE87 pKa = 4.27AVRR90 pKa = 11.84FTARR94 pKa = 11.84VFATEE99 pKa = 4.03VLMAAYY105 pKa = 10.1DD106 pKa = 3.69SAHH109 pKa = 6.37QIGLPMQLMGLDD121 pKa = 3.7VQQALQMLYY130 pKa = 11.07GDD132 pKa = 5.33LDD134 pKa = 3.54HH135 pKa = 7.6TDD137 pKa = 3.55PQRR140 pKa = 11.84VTDD143 pKa = 4.08AEE145 pKa = 4.27TAAFFAAVGGDD156 pKa = 4.04DD157 pKa = 3.89EE158 pKa = 5.28DD159 pKa = 3.86

Molecular weight: 17.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R4A196|A0A2R4A196_9CAUD Uncharacterized protein OS=Microbacterium phage Elva OX=2126929 GN=26 PE=4 SV=1
MM1 pKa = 7.32SAQVFLIEE9 pKa = 4.69GADD12 pKa = 3.81GTGKK16 pKa = 7.22TTFVGRR22 pKa = 11.84MAARR26 pKa = 11.84HH27 pKa = 5.21AAAGHH32 pKa = 5.99RR33 pKa = 11.84EE34 pKa = 3.59PRR36 pKa = 11.84LIHH39 pKa = 6.66NDD41 pKa = 3.28ASDD44 pKa = 3.24HH45 pKa = 6.18RR46 pKa = 11.84LPGSLYY52 pKa = 8.97RR53 pKa = 11.84HH54 pKa = 5.92YY55 pKa = 10.58RR56 pKa = 11.84AQLLDD61 pKa = 3.71AVDD64 pKa = 4.54FRR66 pKa = 11.84DD67 pKa = 3.6TAGVTTYY74 pKa = 10.35IDD76 pKa = 3.29RR77 pKa = 11.84SFLSEE82 pKa = 4.2LVYY85 pKa = 11.03GPLYY89 pKa = 10.25RR90 pKa = 11.84GRR92 pKa = 11.84SRR94 pKa = 11.84ITPRR98 pKa = 11.84QARR101 pKa = 11.84RR102 pKa = 11.84LEE104 pKa = 4.12RR105 pKa = 11.84LADD108 pKa = 3.61RR109 pKa = 11.84HH110 pKa = 5.72GVVLLGMTAEE120 pKa = 4.26LNTRR124 pKa = 11.84RR125 pKa = 11.84LRR127 pKa = 11.84VRR129 pKa = 11.84ARR131 pKa = 11.84GEE133 pKa = 3.32AWTAKK138 pKa = 10.13DD139 pKa = 3.3AFVGAEE145 pKa = 3.8YY146 pKa = 10.6SRR148 pKa = 11.84QFRR151 pKa = 11.84EE152 pKa = 3.7SGRR155 pKa = 11.84WVIADD160 pKa = 3.42STSAHH165 pKa = 5.7VSNN168 pKa = 5.11

Molecular weight: 18.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

13211

29

876

186.1

20.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.528 ± 0.282

0.477 ± 0.094

6.29 ± 0.267

6.298 ± 0.251

3.111 ± 0.168

8.72 ± 0.311

1.771 ± 0.145

3.966 ± 0.191

4.231 ± 0.278

8.372 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.263 ± 0.134

2.998 ± 0.234

5.261 ± 0.24

3.474 ± 0.192

6.389 ± 0.431

5.458 ± 0.311

6.51 ± 0.348

8.304 ± 0.305

2.029 ± 0.163

2.551 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski