Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Cellulomonadaceae; Cellulomonas; Cellulomonas fimi

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3760 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4H157|F4H157_CELFA Uncharacterized protein OS=Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) OX=590998 GN=Celf_3312 PE=4 SV=1
MM1 pKa = 7.19STTPDD6 pKa = 3.59GNDD9 pKa = 2.56WTAPGSPGKK18 pKa = 9.27PAATGGVGPAEE29 pKa = 4.48GSAPQTPGTDD39 pKa = 3.2VSADD43 pKa = 3.52SAPDD47 pKa = 3.26ARR49 pKa = 11.84ATGDD53 pKa = 3.84APVGAYY59 pKa = 7.81PSPAPYY65 pKa = 10.05AAPTGDD71 pKa = 3.78PAASGYY77 pKa = 10.34AAAAGHH83 pKa = 6.5GAPAGHH89 pKa = 7.09GAPAGYY95 pKa = 7.61GTPAGYY101 pKa = 9.34GAPAGYY107 pKa = 9.1GAPHH111 pKa = 7.02ATPTAYY117 pKa = 10.12GAAGPYY123 pKa = 10.42ARR125 pKa = 11.84TADD128 pKa = 3.78GGTSDD133 pKa = 4.33KK134 pKa = 11.39SFVAAWLLSLFLGTLGVDD152 pKa = 3.47RR153 pKa = 11.84FYY155 pKa = 11.11LGKK158 pKa = 10.59VGTGILKK165 pKa = 10.53LVTCGGLGVWAFVDD179 pKa = 4.1LLLVLTGSMRR189 pKa = 11.84DD190 pKa = 3.52TQGRR194 pKa = 11.84PLAGYY199 pKa = 10.02EE200 pKa = 4.11DD201 pKa = 4.03TKK203 pKa = 9.72KK204 pKa = 8.96TAWIVTGAAYY214 pKa = 9.45GASLLLGVVGGFAQLAAMAVVAANMPDD241 pKa = 3.58VVQEE245 pKa = 3.94DD246 pKa = 3.62SGYY249 pKa = 11.48GDD251 pKa = 6.29DD252 pKa = 5.43LDD254 pKa = 4.7DD255 pKa = 3.91WPAVEE260 pKa = 4.88EE261 pKa = 4.15PSPTEE266 pKa = 3.58EE267 pKa = 4.44APVVDD272 pKa = 4.69LTAAQWADD280 pKa = 3.33QEE282 pKa = 4.51FGTFEE287 pKa = 4.25VATHH291 pKa = 6.12SGVGDD296 pKa = 3.6AVVPLPAGAVGGLVTATHH314 pKa = 6.49DD315 pKa = 4.01GEE317 pKa = 4.59WNFVIDD323 pKa = 3.95VVDD326 pKa = 3.92AAGQPTGDD334 pKa = 3.68LVVNTIGAYY343 pKa = 10.06SGATAFGLSSVDD355 pKa = 3.39GGTSLQVVADD365 pKa = 4.31GAWTISVAPISSAPVLPPSGAGDD388 pKa = 3.89GVFLYY393 pKa = 10.61DD394 pKa = 4.01GEE396 pKa = 4.46EE397 pKa = 3.95ATFTGAHH404 pKa = 6.56DD405 pKa = 3.94GEE407 pKa = 5.13EE408 pKa = 4.12NFQVSQSRR416 pKa = 11.84AEE418 pKa = 4.12PPWWDD423 pKa = 3.62LPVNEE428 pKa = 4.82IGPWSGPLTLGSGPSVVEE446 pKa = 4.17VVADD450 pKa = 4.14GGWTLTTGG458 pKa = 3.31

Molecular weight: 45.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4H0T5|F4H0T5_CELFA Regulatory protein TetR OS=Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) OX=590998 GN=Celf_2054 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3760

0

3760

1274721

30

2070

339.0

35.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.812 ± 0.063

0.539 ± 0.01

6.717 ± 0.038

5.024 ± 0.039

2.44 ± 0.024

9.39 ± 0.037

2.149 ± 0.019

2.388 ± 0.032

1.266 ± 0.025

10.291 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.407 ± 0.015

1.376 ± 0.021

6.236 ± 0.04

2.634 ± 0.02

8.05 ± 0.044

4.676 ± 0.03

6.539 ± 0.041

10.712 ± 0.053

1.586 ± 0.021

1.772 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski