Ferret coronavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Minacovirus

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A172B201|A0A172B201_9ALPC 3C-like proteinase OS=Ferret coronavirus OX=1264898 GN=orf1ab PE=3 SV=1
MM1 pKa = 7.76FEE3 pKa = 3.53MRR5 pKa = 11.84TPVFLTVCIVLLQYY19 pKa = 10.04AHH21 pKa = 7.7CDD23 pKa = 3.25TFNCSTLHH31 pKa = 6.06NTTLWATDD39 pKa = 3.21QTRR42 pKa = 11.84LLDD45 pKa = 3.83YY46 pKa = 10.4FIANYY51 pKa = 9.97SSRR54 pKa = 11.84LPTGASVVLGDD65 pKa = 4.08YY66 pKa = 10.67FPTLGPWYY74 pKa = 9.74DD75 pKa = 3.74CVSNTTYY82 pKa = 10.99GGVVLEE88 pKa = 4.91DD89 pKa = 3.64LRR91 pKa = 11.84ALYY94 pKa = 10.36LDD96 pKa = 3.87HH97 pKa = 6.27QQGVARR103 pKa = 11.84DD104 pKa = 3.56LAFAVYY110 pKa = 9.27GYY112 pKa = 9.21PYY114 pKa = 9.57TAIVVLNKK122 pKa = 10.26EE123 pKa = 4.23LFNGQGYY130 pKa = 10.42GGLLCFCNGNSTIPAFNANCSTSCTVRR157 pKa = 11.84SFRR160 pKa = 11.84LCADD164 pKa = 3.34EE165 pKa = 5.13SICHH169 pKa = 5.41EE170 pKa = 5.0RR171 pKa = 11.84ILGLKK176 pKa = 8.26WSSSEE181 pKa = 3.92VVAYY185 pKa = 10.14LAGEE189 pKa = 4.35VYY191 pKa = 10.35SYY193 pKa = 11.35KK194 pKa = 10.73LSNHH198 pKa = 6.35WYY200 pKa = 9.45NNVTIKK206 pKa = 9.41TQTNANQIYY215 pKa = 8.12WWFNPVRR222 pKa = 11.84DD223 pKa = 3.67LSYY226 pKa = 10.96YY227 pKa = 10.35NVNKK231 pKa = 9.63TVDD234 pKa = 3.4STIVVSNCTSDD245 pKa = 3.43CSGYY249 pKa = 10.39AANIFAVEE257 pKa = 4.12TGGFIPSSFSFNNWFVLTNSSTIVSGKK284 pKa = 9.78FVSSQPLLVNCLTPVPSFGDD304 pKa = 3.31EE305 pKa = 3.8TSIINFDD312 pKa = 4.06TVPSQCNGATVNGSFDD328 pKa = 3.49VVRR331 pKa = 11.84FNLNFTANVASSSGTSFLALNTTGGVVLYY360 pKa = 8.29LSCFNEE366 pKa = 4.25TKK368 pKa = 8.19TTNLYY373 pKa = 9.43MSEE376 pKa = 4.06GALPFGTHH384 pKa = 6.29EE385 pKa = 4.34GALYY389 pKa = 10.53CYY391 pKa = 10.38VSYY394 pKa = 11.56NEE396 pKa = 4.29TSFKK400 pKa = 10.75YY401 pKa = 10.14IGVLPPSVKK410 pKa = 10.29EE411 pKa = 3.65IAVSKK416 pKa = 10.09WGFVYY421 pKa = 10.59INGYY425 pKa = 10.63NYY427 pKa = 10.16FQTFPIDD434 pKa = 3.43SVAFNLTTGNSGAFWTIAYY453 pKa = 7.01TTFTDD458 pKa = 3.4VLLEE462 pKa = 4.21VSDD465 pKa = 3.99TQIKK469 pKa = 10.22SVTYY473 pKa = 10.18CNSHH477 pKa = 6.99INDD480 pKa = 3.81IKK482 pKa = 10.42CSQMSEE488 pKa = 4.0NLPDD492 pKa = 3.23GFYY495 pKa = 10.52PVSQHH500 pKa = 6.53SLPNVNKK507 pKa = 9.6TFVTLPANFEE517 pKa = 4.14HH518 pKa = 6.76TSINVTGNVLLIYY531 pKa = 9.48YY532 pKa = 7.1AHH534 pKa = 6.84PVFRR538 pKa = 11.84SGNVTLHH545 pKa = 5.83PQGTQTICVNTTQFTFNFDD564 pKa = 4.0SQCHH568 pKa = 4.18QLAGGGCDD576 pKa = 3.81GIGASMISIDD586 pKa = 4.92SGNCPFSPDD595 pKa = 4.12KK596 pKa = 11.18LNQHH600 pKa = 6.75LAFEE604 pKa = 4.49TLCFSTLPTGSDD616 pKa = 3.54CAFSLTVQNRR626 pKa = 11.84YY627 pKa = 5.17FTRR630 pKa = 11.84VFAHH634 pKa = 6.68LFVTYY639 pKa = 10.22KK640 pKa = 10.79YY641 pKa = 10.67GLDD644 pKa = 3.38HH645 pKa = 7.22LGVQTPDD652 pKa = 3.11VGVKK656 pKa = 10.15DD657 pKa = 4.02LSVVYY662 pKa = 9.85QNVCTEE668 pKa = 3.84YY669 pKa = 10.84NIYY672 pKa = 10.17GHH674 pKa = 7.04AGVGIIRR681 pKa = 11.84STNQTLLGGLYY692 pKa = 8.33YY693 pKa = 10.54TSLSGDD699 pKa = 3.35LLGFKK704 pKa = 10.35NVTTGEE710 pKa = 4.27VYY712 pKa = 10.74SIVPCQLSAQAAVINGKK729 pKa = 9.35IVGAVTSVQSPILDD743 pKa = 3.96LPHH746 pKa = 7.16HH747 pKa = 6.41IVTPQFYY754 pKa = 9.64YY755 pKa = 10.62HH756 pKa = 7.09SIYY759 pKa = 10.65NYY761 pKa = 9.87SATPTSYY768 pKa = 9.3RR769 pKa = 11.84TNNGFDD775 pKa = 3.47KK776 pKa = 11.17YY777 pKa = 10.98LVNCTPIITYY787 pKa = 10.93SNMGVCEE794 pKa = 3.87NGALVFINITQSEE807 pKa = 4.64NPVQPISTGNVTIPSNFTISVQVEE831 pKa = 3.96YY832 pKa = 11.03LQMSSEE838 pKa = 4.25AVSIDD843 pKa = 3.18CAQYY847 pKa = 10.24VCNGNPRR854 pKa = 11.84CNRR857 pKa = 11.84LLAQYY862 pKa = 10.42ISACHH867 pKa = 6.55AIEE870 pKa = 3.9QALITSTRR878 pKa = 11.84LEE880 pKa = 4.05ALEE883 pKa = 4.53LEE885 pKa = 4.52SMISISDD892 pKa = 3.57NALALATVEE901 pKa = 4.52TFNSSDD907 pKa = 3.63YY908 pKa = 11.15LDD910 pKa = 3.95PVYY913 pKa = 10.96NDD915 pKa = 3.1QHH917 pKa = 5.3NTIGGIYY924 pKa = 9.08MDD926 pKa = 4.39GLKK929 pKa = 10.72DD930 pKa = 3.47LLPRR934 pKa = 11.84RR935 pKa = 11.84SCANKK940 pKa = 9.99HH941 pKa = 4.29GTCRR945 pKa = 11.84SVIEE949 pKa = 3.89EE950 pKa = 4.11LLFNKK955 pKa = 9.48VVTSGLGTVDD965 pKa = 3.79EE966 pKa = 4.9DD967 pKa = 4.87YY968 pKa = 11.24KK969 pKa = 11.15RR970 pKa = 11.84CTNGLDD976 pKa = 3.62IADD979 pKa = 4.62LVCAQYY985 pKa = 11.58YY986 pKa = 8.99NGIMVLPGVVNADD999 pKa = 3.0KK1000 pKa = 10.45MAMYY1004 pKa = 8.05TASLAGGITLGALGGGLVSVPFATAVQARR1033 pKa = 11.84LNYY1036 pKa = 9.72VALQTDD1042 pKa = 4.22VLQQNQKK1049 pKa = 9.71ILAASFNQAIGNITLAFGKK1068 pKa = 10.32VNSAIQQTAQGLSTVAQALTKK1089 pKa = 10.33VQDD1092 pKa = 3.72VVNSQGKK1099 pKa = 9.12ALNHH1103 pKa = 5.94LTAQLQNNFQAISSSIEE1120 pKa = 3.99DD1121 pKa = 3.12IYY1123 pKa = 11.76YY1124 pKa = 10.95KK1125 pKa = 10.47LDD1127 pKa = 3.56EE1128 pKa = 4.62VNADD1132 pKa = 3.46AQVDD1136 pKa = 3.81RR1137 pKa = 11.84LITGRR1142 pKa = 11.84LASLNAFVTQTLTNQAQIRR1161 pKa = 11.84ASRR1164 pKa = 11.84QLSKK1168 pKa = 11.24EE1169 pKa = 4.03KK1170 pKa = 10.5INEE1173 pKa = 4.27CVRR1176 pKa = 11.84SQSSRR1181 pKa = 11.84FGFCGNGTHH1190 pKa = 7.49LFSLANAAPRR1200 pKa = 11.84GVMLFHH1206 pKa = 6.51TVLLPTSYY1214 pKa = 9.07KK1215 pKa = 10.18TVTAWSGVCAISNNKK1230 pKa = 8.2TMGFIVKK1237 pKa = 9.91DD1238 pKa = 3.55VSLTLFKK1245 pKa = 11.26NHH1247 pKa = 7.23DD1248 pKa = 3.99DD1249 pKa = 3.9KK1250 pKa = 11.61FYY1252 pKa = 9.47LTPRR1256 pKa = 11.84TMYY1259 pKa = 9.32EE1260 pKa = 3.65PRR1262 pKa = 11.84VATMSDD1268 pKa = 3.53FVQIEE1273 pKa = 4.54SCTTTFVNATVAEE1286 pKa = 4.51LPSIIPDD1293 pKa = 3.78YY1294 pKa = 11.1IDD1296 pKa = 3.31INGTIKK1302 pKa = 11.04DD1303 pKa = 3.94MLDD1306 pKa = 3.04QYY1308 pKa = 10.89KK1309 pKa = 10.44PNWTVPEE1316 pKa = 3.94LTIDD1320 pKa = 3.94VFNQTYY1326 pKa = 10.97LNLTGEE1332 pKa = 4.32INEE1335 pKa = 4.23LEE1337 pKa = 4.13NRR1339 pKa = 11.84SVILQQTVLEE1349 pKa = 4.5LEE1351 pKa = 4.27SLIDD1355 pKa = 4.28NINGTLVNLEE1365 pKa = 3.71WLNRR1369 pKa = 11.84VEE1371 pKa = 5.64TFVKK1375 pKa = 8.87WPWWVWVIIGLILLIALPMLLFCCLSTGCCGCCGCLTSCLAGCCKK1420 pKa = 10.33NSCKK1424 pKa = 10.19RR1425 pKa = 11.84PSYY1428 pKa = 9.43YY1429 pKa = 10.55EE1430 pKa = 3.75PMEE1433 pKa = 4.32KK1434 pKa = 10.25VHH1436 pKa = 7.08INN1438 pKa = 3.15

Molecular weight: 157.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A172B5K4|A0A172B5K4_9ALPC Envelope small membrane protein OS=Ferret coronavirus OX=1264898 GN=E PE=3 SV=1
MM1 pKa = 7.48SSSLITIFSGKK12 pKa = 8.72IWFSLSRR19 pKa = 11.84PFKK22 pKa = 10.06DD23 pKa = 2.98WIVSKK28 pKa = 11.02VRR30 pKa = 11.84FKK32 pKa = 10.96TPVGGKK38 pKa = 9.67VKK40 pKa = 10.48LDD42 pKa = 3.27YY43 pKa = 10.5RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84ALLNSHH52 pKa = 6.23NNHH55 pKa = 5.52VNSNFNSSVSVLKK68 pKa = 9.88FLRR71 pKa = 11.84ARR73 pKa = 11.84RR74 pKa = 11.84WQASTSHH81 pKa = 5.81IQLGKK86 pKa = 10.2IRR88 pKa = 11.84VFF90 pKa = 3.62

Molecular weight: 10.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

1

9

13279

82

6622

1475.4

164.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.461 ± 0.26

3.276 ± 0.357

5.543 ± 0.461

3.961 ± 0.211

5.452 ± 0.264

6.657 ± 0.233

1.86 ± 0.078

4.654 ± 0.46

5.859 ± 0.677

8.962 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.47 ± 0.327

5.889 ± 0.452

3.457 ± 0.191

2.734 ± 0.372

3.268 ± 0.493

7.214 ± 0.541

5.874 ± 0.62

10.264 ± 0.657

1.205 ± 0.226

4.94 ± 0.276
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski