Hubei virga-like virus 9

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KK45|A0A1L3KK45_9VIRU Uncharacterized protein OS=Hubei virga-like virus 9 OX=1923342 PE=4 SV=1
MM1 pKa = 7.64CEE3 pKa = 4.39KK4 pKa = 10.24SDD6 pKa = 3.48SGIIGEE12 pKa = 4.09EE13 pKa = 4.1SYY15 pKa = 11.21GIEE18 pKa = 3.37RR19 pKa = 11.84LLEE22 pKa = 4.0NEE24 pKa = 4.68FIHH27 pKa = 7.32YY28 pKa = 10.75DD29 pKa = 4.09LDD31 pKa = 4.79DD32 pKa = 4.3KK33 pKa = 11.26LQQRR37 pKa = 11.84LVITLLLTNSWRR49 pKa = 11.84SDD51 pKa = 4.34PIWLLQKK58 pKa = 9.16ITAARR63 pKa = 11.84KK64 pKa = 7.93RR65 pKa = 11.84DD66 pKa = 3.39TYY68 pKa = 11.51SFYY71 pKa = 10.3EE72 pKa = 4.18SRR74 pKa = 11.84WFEE77 pKa = 3.97IEE79 pKa = 4.88AGGQQATTDD88 pKa = 3.6GSYY91 pKa = 11.08QDD93 pKa = 3.49NTEE96 pKa = 3.76EE97 pKa = 4.98GEE99 pKa = 4.15THH101 pKa = 6.33KK102 pKa = 10.63RR103 pKa = 11.84GRR105 pKa = 11.84YY106 pKa = 9.41DD107 pKa = 4.84DD108 pKa = 4.17YY109 pKa = 11.88SCC111 pKa = 5.25

Molecular weight: 13.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJW2|A0A1L3KJW2_9VIRU Uncharacterized protein OS=Hubei virga-like virus 9 OX=1923342 PE=4 SV=1
MM1 pKa = 7.21SHH3 pKa = 6.39GGSRR7 pKa = 11.84SRR9 pKa = 11.84QAANKK14 pKa = 9.42RR15 pKa = 11.84RR16 pKa = 11.84QTGHH20 pKa = 6.22TKK22 pKa = 10.05TIPKK26 pKa = 9.82RR27 pKa = 11.84VRR29 pKa = 11.84HH30 pKa = 5.07TSEE33 pKa = 3.93GDD35 pKa = 3.22TTIILADD42 pKa = 3.85SPEE45 pKa = 4.39GTSTRR50 pKa = 11.84TPSKK54 pKa = 9.95RR55 pKa = 11.84ARR57 pKa = 11.84HH58 pKa = 5.24VSASATITTPIEE70 pKa = 4.0TPEE73 pKa = 4.13EE74 pKa = 4.51YY75 pKa = 9.01NTSNLTVDD83 pKa = 4.68PSPSSPEE90 pKa = 3.81PQFTPPSAIVSRR102 pKa = 11.84SVRR105 pKa = 11.84IRR107 pKa = 11.84GRR109 pKa = 11.84SLDD112 pKa = 3.57MTTPKK117 pKa = 10.48YY118 pKa = 10.31EE119 pKa = 3.74RR120 pKa = 11.84WTSRR124 pKa = 11.84MEE126 pKa = 4.14RR127 pKa = 11.84YY128 pKa = 8.38TNANFMDD135 pKa = 3.88AVIVEE140 pKa = 4.23DD141 pKa = 3.91TLRR144 pKa = 11.84EE145 pKa = 3.92MSVRR149 pKa = 11.84NYY151 pKa = 10.28SNRR154 pKa = 11.84PARR157 pKa = 11.84EE158 pKa = 3.81SALTSIVSMNGDD170 pKa = 3.91RR171 pKa = 11.84IVGPTLRR178 pKa = 11.84FPDD181 pKa = 3.44TGVYY185 pKa = 9.99ILVSTGPLRR194 pKa = 11.84EE195 pKa = 4.57LFDD198 pKa = 4.59QIILACTLTEE208 pKa = 4.21SDD210 pKa = 4.05PTTSSGSAHH219 pKa = 6.25NRR221 pKa = 11.84YY222 pKa = 9.97DD223 pKa = 3.89DD224 pKa = 4.34NKK226 pKa = 10.3LSYY229 pKa = 10.92YY230 pKa = 10.32KK231 pKa = 10.53AISNALFLLRR241 pKa = 11.84RR242 pKa = 11.84RR243 pKa = 11.84FEE245 pKa = 4.38PAAHH249 pKa = 6.38VYY251 pKa = 10.36DD252 pKa = 3.68IYY254 pKa = 11.45SRR256 pKa = 11.84STFEE260 pKa = 3.74STNRR264 pKa = 11.84IVWNDD269 pKa = 3.0GNTTRR274 pKa = 11.84QQ275 pKa = 3.13

Molecular weight: 30.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3460

111

2947

865.0

98.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.04 ± 1.061

2.312 ± 0.79

7.254 ± 0.659

5.405 ± 0.972

3.757 ± 0.697

4.798 ± 0.441

2.688 ± 0.336

5.145 ± 0.434

5.867 ± 0.944

8.41 ± 0.872

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.642 ± 0.602

4.191 ± 0.376

4.104 ± 1.348

2.312 ± 0.57

6.821 ± 0.93

7.081 ± 1.225

6.908 ± 1.482

7.63 ± 2.14

1.04 ± 0.3

4.595 ± 0.484
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski