Desulfosporosinus fructosivorans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfosporosinus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5570 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Z0QZI7|A0A4Z0QZI7_9FIRM Ferredoxin family protein OS=Desulfosporosinus fructosivorans OX=2018669 GN=E4K67_25505 PE=4 SV=1
MM1 pKa = 6.88MRR3 pKa = 11.84ALFAGVSGLQAHH15 pKa = 5.45QKK17 pKa = 10.4KK18 pKa = 9.42MDD20 pKa = 3.81VIGNNIANVNTVAYY34 pKa = 8.5KK35 pKa = 10.33ASRR38 pKa = 11.84VTFSDD43 pKa = 3.62VLSQTLSKK51 pKa = 10.06ATAANSDD58 pKa = 3.88SGVGGTNPKK67 pKa = 10.34QIGLGVGVASIDD79 pKa = 3.43MLMTDD84 pKa = 4.67GGTEE88 pKa = 4.12STGNTTDD95 pKa = 4.58LSLEE99 pKa = 4.01GDD101 pKa = 3.66GFFIVRR107 pKa = 11.84NGVTGSYY114 pKa = 9.1MFTRR118 pKa = 11.84AGDD121 pKa = 3.53FTVDD125 pKa = 3.12EE126 pKa = 4.96KK127 pKa = 11.91GNLVTSDD134 pKa = 2.91GMNVYY139 pKa = 9.44GWSSYY144 pKa = 7.4STNAEE149 pKa = 3.27GDD151 pKa = 3.91YY152 pKa = 11.4EE153 pKa = 4.84FDD155 pKa = 3.2TDD157 pKa = 4.86NEE159 pKa = 4.18VEE161 pKa = 4.77AINIYY166 pKa = 10.97ADD168 pKa = 3.62AYY170 pKa = 10.66NGNKK174 pKa = 9.89KK175 pKa = 9.63ILSAQATEE183 pKa = 4.04NVVFSGSLDD192 pKa = 3.5SSEE195 pKa = 4.36EE196 pKa = 4.13AIEE199 pKa = 6.04DD200 pKa = 3.48IDD202 pKa = 6.34DD203 pKa = 4.09DD204 pKa = 4.49TEE206 pKa = 4.14PQYY209 pKa = 8.29TTSMTVYY216 pKa = 10.31DD217 pKa = 3.95SLGNEE222 pKa = 4.25HH223 pKa = 7.27EE224 pKa = 4.34LTVNYY229 pKa = 9.76IKK231 pKa = 10.73SDD233 pKa = 3.45TNTWDD238 pKa = 3.36CYY240 pKa = 7.42VTYY243 pKa = 10.66DD244 pKa = 4.31SGEE247 pKa = 4.04TDD249 pKa = 3.33TEE251 pKa = 3.94GDD253 pKa = 4.0AILTRR258 pKa = 11.84VDD260 pKa = 2.92IGEE263 pKa = 4.27LQFDD267 pKa = 4.06EE268 pKa = 4.92YY269 pKa = 11.54GDD271 pKa = 3.65IVEE274 pKa = 5.91DD275 pKa = 3.67DD276 pKa = 3.35TSYY279 pKa = 8.59PTTQVLTITPTTGATDD295 pKa = 4.08LEE297 pKa = 4.34ITLDD301 pKa = 3.81FTNLSTGADD310 pKa = 3.76DD311 pKa = 4.38SSVEE315 pKa = 3.85AGEE318 pKa = 3.79IDD320 pKa = 4.44GYY322 pKa = 11.51SSGTLEE328 pKa = 5.91DD329 pKa = 3.97ISIDD333 pKa = 3.61SNGIIMGVYY342 pKa = 10.42SNGSQQPLGMIGIAQFANSSGLQKK366 pKa = 9.77TGSGYY371 pKa = 11.2YY372 pKa = 10.08SATANSGQFTNGVSANGAISSGTLEE397 pKa = 4.2MSNVDD402 pKa = 3.48LSYY405 pKa = 10.98EE406 pKa = 4.48FSQMITTQRR415 pKa = 11.84GFQANSTLITTADD428 pKa = 3.47EE429 pKa = 4.1MLEE432 pKa = 3.93TLINMKK438 pKa = 10.29RR439 pKa = 3.25

Molecular weight: 46.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Z0R4W4|A0A4Z0R4W4_9FIRM Protein-glutamate O-methyltransferase OS=Desulfosporosinus fructosivorans OX=2018669 GN=E4K67_18385 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 9.19QPKK8 pKa = 8.78NRR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 5.44KK13 pKa = 10.09RR14 pKa = 11.84VHH16 pKa = 5.93GFLSRR21 pKa = 11.84MSTPTGRR28 pKa = 11.84NVIKK32 pKa = 10.5RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.52GRR39 pKa = 11.84KK40 pKa = 8.8KK41 pKa = 10.81LSVV44 pKa = 3.15

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5570

0

5570

1667623

22

2862

299.4

33.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.669 ± 0.041

1.166 ± 0.015

4.924 ± 0.027

6.75 ± 0.033

4.069 ± 0.025

7.452 ± 0.032

1.809 ± 0.014

7.643 ± 0.026

6.109 ± 0.031

10.371 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.763 ± 0.017

4.123 ± 0.024

3.862 ± 0.019

3.669 ± 0.019

4.502 ± 0.024

6.128 ± 0.029

5.426 ± 0.028

7.298 ± 0.028

1.047 ± 0.013

3.221 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski