Ruminococcus flavefaciens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2780 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1K1NZF6|A0A1K1NZF6_RUMFL Acyl carrier protein OS=Ruminococcus flavefaciens OX=1265 GN=SAMN02910280_2291 PE=4 SV=1
MM1 pKa = 7.14IQLPIYY7 pKa = 10.41EE8 pKa = 4.98EE9 pKa = 3.98FAHH12 pKa = 6.51WLDD15 pKa = 6.0DD16 pKa = 3.4ILEE19 pKa = 4.35NNEE22 pKa = 3.87MPEE25 pKa = 3.98EE26 pKa = 4.03TKK28 pKa = 10.8AFCFNLYY35 pKa = 10.1EE36 pKa = 4.37EE37 pKa = 4.74SDD39 pKa = 3.71EE40 pKa = 4.02EE41 pKa = 4.77HH42 pKa = 6.44ICGVQIIAAGEE53 pKa = 3.9YY54 pKa = 10.59DD55 pKa = 4.23PEE57 pKa = 6.17DD58 pKa = 4.43KK59 pKa = 10.98DD60 pKa = 5.22GEE62 pKa = 4.49WACEE66 pKa = 4.0EE67 pKa = 4.23VWSSEE72 pKa = 3.9EE73 pKa = 4.2NIFTVDD79 pKa = 3.12TSDD82 pKa = 4.91EE83 pKa = 4.33EE84 pKa = 4.39DD85 pKa = 3.68TSWAHH90 pKa = 5.02AQEE93 pKa = 5.02LIKK96 pKa = 10.85EE97 pKa = 4.26MIEE100 pKa = 3.83EE101 pKa = 4.01YY102 pKa = 10.69LRR104 pKa = 11.84NGKK107 pKa = 9.99YY108 pKa = 10.21SGILSGAAAVAVGFVDD124 pKa = 4.43GEE126 pKa = 4.26LDD128 pKa = 4.46IISQQ132 pKa = 3.57

Molecular weight: 15.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1K1N6L4|A0A1K1N6L4_RUMFL Leader peptidase (Prepilin peptidase) / N-methyltransferase OS=Ruminococcus flavefaciens OX=1265 GN=SAMN02910280_1770 PE=3 SV=1
MM1 pKa = 7.66SKK3 pKa = 10.53RR4 pKa = 11.84LRR6 pKa = 11.84IAIAAGAPLLVLIAFVLRR24 pKa = 11.84DD25 pKa = 3.63QIVGFARR32 pKa = 11.84LMPPCSFHH40 pKa = 7.4KK41 pKa = 10.27LTGYY45 pKa = 9.11WCTGCGNTRR54 pKa = 11.84STIALLQGHH63 pKa = 7.2IWTAIRR69 pKa = 11.84NNATMPFLSLLLCLLYY85 pKa = 10.28IEE87 pKa = 4.85NFAAIFGRR95 pKa = 11.84DD96 pKa = 3.13IKK98 pKa = 11.01LLPRR102 pKa = 11.84KK103 pKa = 9.47LWIWLIVIGLFVVYY117 pKa = 8.97YY118 pKa = 9.52IVRR121 pKa = 11.84NFIPALAPISS131 pKa = 3.7

Molecular weight: 14.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2780

0

2780

979500

37

3739

352.3

39.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.894 ± 0.05

1.643 ± 0.019

6.42 ± 0.037

7.033 ± 0.047

4.308 ± 0.037

7.064 ± 0.054

1.584 ± 0.019

7.327 ± 0.047

7.024 ± 0.04

8.091 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.806 ± 0.024

4.803 ± 0.041

3.28 ± 0.027

2.526 ± 0.032

4.051 ± 0.038

6.56 ± 0.042

5.943 ± 0.063

6.519 ± 0.036

0.88 ± 0.016

4.244 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski