Alfalfa virus S

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Allexivirus

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9PY60|A0A1X9PY60_9VIRU CP OS=Alfalfa virus S OX=1985968 PE=4 SV=1
MM1 pKa = 7.04QAPEE5 pKa = 4.45LVHH8 pKa = 5.77THH10 pKa = 6.91ASTSCQQSPWYY21 pKa = 9.57SSPWALLLVSGLSALLVLAAANYY44 pKa = 8.73FNSLPGSHH52 pKa = 6.55SCLLTITGHH61 pKa = 5.2SVSVSGCEE69 pKa = 3.93NHH71 pKa = 7.13DD72 pKa = 3.49VPAIIQSFAWSGHH85 pKa = 4.1AFNRR89 pKa = 11.84QLPGVIHH96 pKa = 7.05SDD98 pKa = 3.1

Molecular weight: 10.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9PY58|A0A1X9PY58_9VIRU Movement protein TGBp3 OS=Alfalfa virus S OX=1985968 PE=3 SV=1
MM1 pKa = 7.4SFAPPPDD8 pKa = 3.5YY9 pKa = 11.21TKK11 pKa = 11.02VFLAATIGAALALTTLFATRR31 pKa = 11.84NTAPHH36 pKa = 6.03VGDD39 pKa = 4.8NIHH42 pKa = 6.57HH43 pKa = 6.79LPHH46 pKa = 6.7GGLYY50 pKa = 9.86QDD52 pKa = 3.5GNKK55 pKa = 9.9RR56 pKa = 11.84IAYY59 pKa = 8.27AGPGTGAHH67 pKa = 6.57PRR69 pKa = 11.84QHH71 pKa = 6.95FLPAIAVVLLTLGIIISEE89 pKa = 4.11RR90 pKa = 11.84FKK92 pKa = 11.14RR93 pKa = 11.84PTRR96 pKa = 11.84SCRR99 pKa = 11.84CC100 pKa = 3.32

Molecular weight: 10.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2722

98

1657

453.7

50.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.56 ± 0.541

1.763 ± 0.232

5.253 ± 1.043

5.547 ± 0.774

4.225 ± 0.337

4.482 ± 0.576

3.674 ± 0.61

4.923 ± 0.57

4.592 ± 1.266

10.029 ± 0.736

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.874 ± 0.496

4.482 ± 0.707

6.392 ± 0.477

5.29 ± 0.492

5.547 ± 0.455

6.613 ± 1.414

7.788 ± 0.726

4.96 ± 0.259

0.735 ± 0.219

3.27 ± 0.585
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski