Coniella lustricola

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Diaporthales; Schizoparmaceae; Coniella

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11309 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2T3A7R7|A0A2T3A7R7_9PEZI Uncharacterized protein OS=Coniella lustricola OX=2025994 GN=BD289DRAFT_434149 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 9.48FTGSRR7 pKa = 11.84NGLAAAAAALAILQSQVAASPILRR31 pKa = 11.84LHH33 pKa = 6.62RR34 pKa = 11.84KK35 pKa = 8.61SATSIPPFAITPTSSILSSSLTSQPALSMPNGSPAASYY73 pKa = 10.8VAFGLEE79 pKa = 3.89IPITGITGTNPEE91 pKa = 4.13QVFLGSEE98 pKa = 4.19SYY100 pKa = 11.41LLFVQQEE107 pKa = 4.28MTLSADD113 pKa = 3.23VSLNMSNNLLSSSVDD128 pKa = 3.85PIVGFHH134 pKa = 6.74ASSGFSSSSSSVSSSSSSISNSSAGSASDD163 pKa = 4.04LSLLVVLVYY172 pKa = 11.08NNQSTNATSSLNYY185 pKa = 10.2LNQTSQSRR193 pKa = 11.84QFDD196 pKa = 3.67WTSWAASIAADD207 pKa = 3.29SSLRR211 pKa = 11.84MNFDD215 pKa = 3.04MDD217 pKa = 4.17TFASQAGLGKK227 pKa = 10.24AVASEE232 pKa = 4.21FVMITQDD239 pKa = 3.18EE240 pKa = 4.73SSGSGSASYY249 pKa = 10.88SAVTTLSVGAGGASQTAAAVFLTTGLPSAAANGIPTMGAASAAVGVNGMASASLGSGSSAMATGAAAQGGYY320 pKa = 10.64AFASAASGSSATGAAWASSSDD341 pKa = 3.64SSSTSTDD348 pKa = 3.19SNVGVAINNLVSAMSSSMSSIDD370 pKa = 3.79SGVLNSDD377 pKa = 3.23SSDD380 pKa = 3.63NLGSAASGTASMAGGAAVALPTGFASTSLAGSTSSFASSNISSSTSSPTPGTCVAGIYY438 pKa = 9.9KK439 pKa = 10.47CSLDD443 pKa = 3.27GSSYY447 pKa = 8.03QQCASGGTWSVSMAMAAGTTCTPGVSSGLSISASPSTSGSLASSSGSATLSGLSSMDD504 pKa = 3.23SSSDD508 pKa = 3.42SSSSATASGMGLSGLGGLVSLKK530 pKa = 10.53SSQPGSASASGMASEE545 pKa = 5.91GNDD548 pKa = 3.2ADD550 pKa = 4.1IGSSDD555 pKa = 4.05SILPKK560 pKa = 10.61GSFSGASGMGSFEE573 pKa = 4.25SSGNSAFSSGSSTLFHH589 pKa = 6.78HH590 pKa = 7.16GKK592 pKa = 10.36GSSAGAKK599 pKa = 9.76DD600 pKa = 3.43SSSFASSGDD609 pKa = 3.59SGSATSFLSGGSSSSGSGDD628 pKa = 3.18LGSFSASDD636 pKa = 3.89YY637 pKa = 11.05SDD639 pKa = 3.31SHH641 pKa = 7.29PSFLMGGSASGASDD655 pKa = 3.45GLGSLASAGEE665 pKa = 4.35SASSIGSSSSSTQSGGSLSFLGGNSSDD692 pKa = 3.84SQSGDD697 pKa = 3.35SLSSVGVPVTATDD710 pKa = 3.65SSISASSPEE719 pKa = 4.13DD720 pKa = 3.3SCDD723 pKa = 3.42EE724 pKa = 4.62DD725 pKa = 3.93SATTTGSGDD734 pKa = 3.5VSSSTSGSQGYY745 pKa = 9.76DD746 pKa = 3.02SPLSGGISMASGSASSASSASSSFGSSYY774 pKa = 10.42GQSGASASSSTASSLAGSSYY794 pKa = 10.08GQSGTSDD801 pKa = 3.05SSLAASSSSSSAVIPYY817 pKa = 10.1FGGSSASGSVDD828 pKa = 3.44LSGLGDD834 pKa = 3.69STVTDD839 pKa = 3.57SSFGSSASSAYY850 pKa = 10.51GNGASSGMALGGGSSSLSSASSSLGAGSTSVDD882 pKa = 3.4TSSSGASISSVSGDD896 pKa = 3.64SYY898 pKa = 11.39STDD901 pKa = 2.96ASSSGNASSSLSGDD915 pKa = 3.31SSSIDD920 pKa = 3.3ASSSGGDD927 pKa = 3.24ASSSGSASSSLSGDD941 pKa = 3.31SSSIDD946 pKa = 3.3ASSSGGDD953 pKa = 3.24ASSSGSASSSLSGDD967 pKa = 3.31SSSIDD972 pKa = 3.3ASSSGGDD979 pKa = 3.24ASSSGSASSSLSGDD993 pKa = 3.41SSSVDD998 pKa = 3.14ASRR1001 pKa = 11.84TGGDD1005 pKa = 3.46ASSMSGDD1012 pKa = 3.47SSIVDD1017 pKa = 3.61ASSSGGDD1024 pKa = 3.03ASSFGSAMSGASTGSGLEE1042 pKa = 4.42DD1043 pKa = 3.97GSSGGSGLGYY1053 pKa = 10.48LSGSGALGASGTAQAQGAQSAMATGGYY1080 pKa = 9.18QGSAFATAAYY1090 pKa = 9.94QSVASGTASASAAVVTGAASNNLAAALGDD1119 pKa = 3.81ALGNMAVTIGLDD1131 pKa = 2.93IDD1133 pKa = 3.6NGEE1136 pKa = 4.35FEE1138 pKa = 4.56TTITANLKK1146 pKa = 10.48DD1147 pKa = 3.73RR1148 pKa = 11.84TVPTPSPVMWDD1159 pKa = 3.52FRR1161 pKa = 11.84KK1162 pKa = 10.62GSLNARR1168 pKa = 11.84ASPAPSSSSSSSSSSSSTVKK1188 pKa = 10.19SAAAGRR1194 pKa = 11.84VQEE1197 pKa = 4.83AGPAKK1202 pKa = 10.01QVVGVVFALCIASLVMM1218 pKa = 5.16

Molecular weight:
114.23 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2T2ZTW6|A0A2T2ZTW6_9PEZI Delta(24)-sterol reductase OS=Coniella lustricola OX=2025994 GN=BD289DRAFT_418248 PE=3 SV=1
MM1 pKa = 7.82AGMLAMLSLVVRR13 pKa = 11.84TLSWRR18 pKa = 11.84PSQTHH23 pKa = 5.28SAAGAAAAGEE33 pKa = 4.62CATKK37 pKa = 8.46TTLSSLHH44 pKa = 5.75GRR46 pKa = 11.84SLRR49 pKa = 11.84RR50 pKa = 11.84SHH52 pKa = 6.16RR53 pKa = 11.84QRR55 pKa = 11.84HH56 pKa = 3.84TRR58 pKa = 11.84ARR60 pKa = 11.84RR61 pKa = 11.84PRR63 pKa = 11.84RR64 pKa = 11.84ATAQTVRR71 pKa = 11.84HH72 pKa = 5.76GWAAQQQQPPPQHH85 pKa = 6.68PPRR88 pKa = 11.84PRR90 pKa = 11.84RR91 pKa = 11.84PRR93 pKa = 11.84TKK95 pKa = 7.53TTRR98 pKa = 11.84RR99 pKa = 11.84TKK101 pKa = 8.27TTTTSSCASACAAALAAAVAGPAMAAATTPKK132 pKa = 9.67RR133 pKa = 11.84AEE135 pKa = 4.01RR136 pKa = 11.84RR137 pKa = 11.84RR138 pKa = 11.84GAQSQSATSSRR149 pKa = 11.84TTARR153 pKa = 11.84GRR155 pKa = 11.84RR156 pKa = 11.84RR157 pKa = 11.84CRR159 pKa = 11.84RR160 pKa = 11.84RR161 pKa = 11.84RR162 pKa = 11.84GAGG165 pKa = 3.04

Molecular weight:
17.85 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11309

0

11309

5000157

49

4995

442.1

48.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.45 ± 0.024

1.343 ± 0.01

5.675 ± 0.016

5.716 ± 0.024

3.562 ± 0.016

6.989 ± 0.021

2.517 ± 0.01

4.497 ± 0.015

4.498 ± 0.019

8.828 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.185 ± 0.009

3.54 ± 0.012

5.997 ± 0.025

4.194 ± 0.019

6.141 ± 0.019

8.448 ± 0.027

6.14 ± 0.017

6.148 ± 0.016

1.479 ± 0.009

2.651 ± 0.012

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski