Phytoactinopolyspora halophila

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Jiangellales; Jiangellaceae; Phytoactinopolyspora

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4405 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A329QS44|A0A329QS44_9ACTN Gamma carbonic anhydrase family protein OS=Phytoactinopolyspora halophila OX=1981511 GN=DPM12_11130 PE=4 SV=1
MM1 pKa = 7.32SRR3 pKa = 11.84RR4 pKa = 11.84LASRR8 pKa = 11.84FSARR12 pKa = 11.84SRR14 pKa = 11.84HH15 pKa = 5.38TDD17 pKa = 3.03RR18 pKa = 11.84QRR20 pKa = 11.84DD21 pKa = 3.56PHH23 pKa = 7.86RR24 pKa = 11.84YY25 pKa = 8.66ASLRR29 pKa = 11.84RR30 pKa = 11.84ASVAAVAATVAGLLVSGAILSAPQGGDD57 pKa = 3.17DD58 pKa = 4.01TADD61 pKa = 3.41STTVRR66 pKa = 11.84VNANGEE72 pKa = 4.23DD73 pKa = 3.96LTDD76 pKa = 3.6STDD79 pKa = 3.6MVWAGDD85 pKa = 3.59QPYY88 pKa = 10.99GSGPWGHH95 pKa = 5.98GTLFGSGSMSGPVTGTDD112 pKa = 3.65DD113 pKa = 4.18DD114 pKa = 4.43EE115 pKa = 6.36LYY117 pKa = 10.83HH118 pKa = 6.87SYY120 pKa = 11.57NLFAAGSGYY129 pKa = 8.89TFDD132 pKa = 5.86LPDD135 pKa = 3.93GDD137 pKa = 4.58YY138 pKa = 10.84EE139 pKa = 4.57VTVKK143 pKa = 9.81TVEE146 pKa = 4.04DD147 pKa = 3.88WATGPGQRR155 pKa = 11.84VFDD158 pKa = 4.0VNVDD162 pKa = 3.61GEE164 pKa = 4.93TVLSEE169 pKa = 3.64FDD171 pKa = 3.35IFAEE175 pKa = 4.7CGASTACDD183 pKa = 3.06RR184 pKa = 11.84SFPASVSGGHH194 pKa = 6.38LSIGFTTNGGSNYY207 pKa = 9.09ATVSAIAITPADD219 pKa = 4.16GGGGDD224 pKa = 4.93PDD226 pKa = 4.18PTPTDD231 pKa = 3.65PTDD234 pKa = 4.25DD235 pKa = 4.11PTPTDD240 pKa = 4.68DD241 pKa = 4.55PTPTDD246 pKa = 4.68DD247 pKa = 4.55PTPTDD252 pKa = 4.61DD253 pKa = 5.15PDD255 pKa = 5.51PGDD258 pKa = 4.08GLPEE262 pKa = 4.31RR263 pKa = 11.84LLTGYY268 pKa = 8.0WHH270 pKa = 6.76NFDD273 pKa = 4.57NNSTVLPLADD283 pKa = 4.02VPDD286 pKa = 4.41SYY288 pKa = 11.98DD289 pKa = 3.38VVAVAFGTEE298 pKa = 4.29DD299 pKa = 3.27VQRR302 pKa = 11.84PGGVDD307 pKa = 3.74FSVSDD312 pKa = 3.98EE313 pKa = 4.32VSDD316 pKa = 4.22SLGGYY321 pKa = 9.75SDD323 pKa = 5.27ADD325 pKa = 3.55LAADD329 pKa = 3.92VEE331 pKa = 4.47QLQEE335 pKa = 3.92RR336 pKa = 11.84GTAVILSIGGEE347 pKa = 4.02RR348 pKa = 11.84GNVRR352 pKa = 11.84VDD354 pKa = 3.3TQEE357 pKa = 4.0RR358 pKa = 11.84ADD360 pKa = 3.83NFADD364 pKa = 3.49SLYY367 pKa = 11.11EE368 pKa = 4.48LMQQYY373 pKa = 10.21GFDD376 pKa = 3.76GVDD379 pKa = 2.85IDD381 pKa = 4.72LEE383 pKa = 4.56HH384 pKa = 7.11GISPAHH390 pKa = 6.35MEE392 pKa = 3.97SALRR396 pKa = 11.84QLADD400 pKa = 3.17RR401 pKa = 11.84VGPEE405 pKa = 4.3LVITMAPQTLDD416 pKa = 3.41MQFTTSDD423 pKa = 3.75YY424 pKa = 11.04FQLALNISDD433 pKa = 3.5ILTVVNTQYY442 pKa = 11.45YY443 pKa = 10.69NSGSMNGCDD452 pKa = 3.39GNVYY456 pKa = 10.5AQGSVEE462 pKa = 5.27FIAALACIQLEE473 pKa = 4.22NGLDD477 pKa = 3.79PDD479 pKa = 3.92QVGLGLPATPSAAGGGYY496 pKa = 10.46VNPTVVNDD504 pKa = 4.74ALDD507 pKa = 3.93CLATGDD513 pKa = 3.68EE514 pKa = 4.98CGAFSPPDD522 pKa = 3.71TYY524 pKa = 11.37PEE526 pKa = 3.62IRR528 pKa = 11.84GAMTWSINWDD538 pKa = 3.1ATNGYY543 pKa = 9.46NFAEE547 pKa = 4.68TVAPHH552 pKa = 6.72LTTLPP557 pKa = 3.64

Molecular weight: 58.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A329QVR9|A0A329QVR9_9ACTN YbhB/YbcL family Raf kinase inhibitor-like protein OS=Phytoactinopolyspora halophila OX=1981511 GN=DPM12_09960 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84SRR14 pKa = 11.84THH16 pKa = 6.18GFRR19 pKa = 11.84LRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84AGRR28 pKa = 11.84AIVSARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.73GRR39 pKa = 11.84SKK41 pKa = 10.12LTAA44 pKa = 4.04

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4405

0

4405

1472675

25

6998

334.3

36.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.308 ± 0.054

0.724 ± 0.009

6.849 ± 0.036

6.427 ± 0.042

2.825 ± 0.023

9.035 ± 0.034

2.388 ± 0.019

4.004 ± 0.023

1.56 ± 0.021

9.708 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.97 ± 0.015

1.921 ± 0.018

5.621 ± 0.024

2.837 ± 0.016

7.733 ± 0.039

5.555 ± 0.022

5.984 ± 0.023

8.908 ± 0.031

1.527 ± 0.017

2.115 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski