Candidatus Erwinia haradaeae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Erwinia

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 630 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A451DPX6|A0A451DPX6_9GAMM 2-iminoacetate synthase OS=Candidatus Erwinia haradaeae OX=1922217 GN=thiH PE=4 SV=1
MM1 pKa = 7.85KK2 pKa = 9.36ITFNNRR8 pKa = 11.84SLEE11 pKa = 4.03LEE13 pKa = 4.2NPLSITNFLTDD24 pKa = 2.86QCYY27 pKa = 10.48EE28 pKa = 4.18SGPGIALSLNQNVIPHH44 pKa = 6.0QYY46 pKa = 8.79WPDD49 pKa = 3.47YY50 pKa = 9.97YY51 pKa = 10.44INDD54 pKa = 3.91GDD56 pKa = 5.91DD57 pKa = 3.16ILLFQAIAGGG67 pKa = 3.54

Molecular weight: 7.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A451DIZ9|A0A451DIZ9_9GAMM Aminotransferase OS=Candidatus Erwinia haradaeae OX=1922217 GN=aspC PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.58RR12 pKa = 11.84NRR14 pKa = 11.84THH16 pKa = 7.31GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 10.13SKK25 pKa = 10.5NGRR28 pKa = 11.84KK29 pKa = 9.04ILSHH33 pKa = 6.45RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.6GRR39 pKa = 11.84VRR41 pKa = 11.84LTVSSNKK48 pKa = 9.58HH49 pKa = 4.31

Molecular weight: 5.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

630

0

630

211832

38

1408

336.2

37.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.893 ± 0.071

1.435 ± 0.035

4.714 ± 0.058

4.973 ± 0.088

3.787 ± 0.057

6.552 ± 0.075

2.917 ± 0.043

8.847 ± 0.084

5.555 ± 0.069

10.596 ± 0.1

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.703 ± 0.035

4.758 ± 0.054

3.716 ± 0.043

4.22 ± 0.052

5.402 ± 0.077

6.811 ± 0.068

5.388 ± 0.05

6.268 ± 0.077

1.159 ± 0.035

3.306 ± 0.057
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski