Roseovarius Plymouth podovirus 1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Rhodovirinae; Plymouthvirus

Average proteome isoelectric point is 5.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4Q596|K4Q596_9CAUD Hypothetical host-like thioredoxin OS=Roseovarius Plymouth podovirus 1 OX=926474 PE=4 SV=1
MM1 pKa = 7.72NDD3 pKa = 3.3LNLTAVMEE11 pKa = 4.2PRR13 pKa = 11.84YY14 pKa = 10.49VLTIQDD20 pKa = 3.27QSVVVGSDD28 pKa = 3.47FNLTLQEE35 pKa = 3.73NSAPLKK41 pKa = 9.95LTLGLQGQKK50 pKa = 10.52GIQGDD55 pKa = 4.06VGPQGPVGPAGPAGTDD71 pKa = 3.28GTDD74 pKa = 3.27GVSFAFDD81 pKa = 3.43GTDD84 pKa = 2.83TFANRR89 pKa = 11.84NLYY92 pKa = 9.81DD93 pKa = 3.8AEE95 pKa = 4.42VVGWRR100 pKa = 11.84FLASDD105 pKa = 3.18TGEE108 pKa = 3.68IYY110 pKa = 10.93VRR112 pKa = 11.84LDD114 pKa = 3.13PTGWSAPIQLKK125 pKa = 10.75GDD127 pKa = 3.77TGPQGPQGIQGVQGPIGPQGNPGADD152 pKa = 3.16GQGFTVDD159 pKa = 4.43AYY161 pKa = 11.01AEE163 pKa = 4.13LVNLGTYY170 pKa = 10.61DD171 pKa = 3.53NEE173 pKa = 4.37AEE175 pKa = 4.34GFSVVDD181 pKa = 3.54PVTGLLYY188 pKa = 10.09IRR190 pKa = 11.84YY191 pKa = 8.73SVAGTWTNGIQFVGPEE207 pKa = 4.54GPTGPIGPAGPTGAPGAAGADD228 pKa = 3.59GADD231 pKa = 3.46FAFDD235 pKa = 3.62AVGTLPEE242 pKa = 4.36KK243 pKa = 10.69NLYY246 pKa = 8.54NAQPEE251 pKa = 4.68GFTFLDD257 pKa = 3.62EE258 pKa = 4.55VNGNFYY264 pKa = 10.71VRR266 pKa = 11.84NDD268 pKa = 3.29ATIGAWTNAIPFQGPTGPQGAQGDD292 pKa = 4.35AGISAYY298 pKa = 10.5DD299 pKa = 3.8LAVSQQGFVGSVSDD313 pKa = 3.5WLTTLTAYY321 pKa = 10.01GVAVSNGFVGTQADD335 pKa = 3.84WLIHH339 pKa = 5.27INAYY343 pKa = 9.9GIAVQNGYY351 pKa = 10.62LGTQAQWLQDD361 pKa = 3.28MTAYY365 pKa = 10.39GIAVLNGYY373 pKa = 10.36AGTEE377 pKa = 4.2PEE379 pKa = 4.09WLIHH383 pKa = 5.28INAYY387 pKa = 9.51GRR389 pKa = 11.84ALDD392 pKa = 3.63NGFVGTEE399 pKa = 4.1LEE401 pKa = 4.28WLDD404 pKa = 3.57SLTAYY409 pKa = 10.04GVAQSNGYY417 pKa = 10.58AGTKK421 pKa = 9.65AAWLTSLTAYY431 pKa = 9.88GVAVDD436 pKa = 3.79NGFVGTEE443 pKa = 4.22PEE445 pKa = 4.17WLTHH449 pKa = 5.25ISAYY453 pKa = 9.82GVAVQNGFVGTEE465 pKa = 4.04LQWLDD470 pKa = 3.89SLVGPIGPQGIQGLKK485 pKa = 10.33GDD487 pKa = 4.51TGQSLIPDD495 pKa = 5.31AIDD498 pKa = 3.87LFANRR503 pKa = 11.84ALYY506 pKa = 8.54DD507 pKa = 3.6TEE509 pKa = 5.35LEE511 pKa = 4.34GFVFMASDD519 pKa = 3.17TGLIYY524 pKa = 10.63FRR526 pKa = 11.84EE527 pKa = 4.4TVFNGTWSVGVPFRR541 pKa = 11.84GPDD544 pKa = 3.68GLPGDD549 pKa = 3.78VTTRR553 pKa = 11.84SLINTATYY561 pKa = 9.25TVLNTDD567 pKa = 3.68LQTGRR572 pKa = 11.84EE573 pKa = 4.02IKK575 pKa = 10.55KK576 pKa = 9.06VDD578 pKa = 3.58HH579 pKa = 6.84AVGCAITVPAGLTNQEE595 pKa = 4.17PCTFIQTNVGQITFAGAVGVTILSADD621 pKa = 3.44SALLSRR627 pKa = 11.84VQGSFVTLLPDD638 pKa = 4.11GDD640 pKa = 4.24TADD643 pKa = 4.47LYY645 pKa = 11.51YY646 pKa = 10.31LTGDD650 pKa = 3.4IVAA653 pKa = 4.9

Molecular weight: 68.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4Q4U2|K4Q4U2_9CAUD N4 gp24-like protein OS=Roseovarius Plymouth podovirus 1 OX=926474 PE=4 SV=1
MM1 pKa = 7.99EE2 pKa = 4.5IRR4 pKa = 11.84EE5 pKa = 3.97YY6 pKa = 10.96RR7 pKa = 11.84QFAEE11 pKa = 4.73ALMSNCYY18 pKa = 10.04LNRR21 pKa = 11.84EE22 pKa = 4.12AQPCKK27 pKa = 10.52LKK29 pKa = 10.63NAKK32 pKa = 8.27TKK34 pKa = 10.3KK35 pKa = 10.07LKK37 pKa = 10.18NLRR40 pKa = 11.84SGIRR44 pKa = 11.84KK45 pKa = 9.13SPYY48 pKa = 9.4LLKK51 pKa = 10.83SIIWKK56 pKa = 8.8EE57 pKa = 3.64QLRR60 pKa = 11.84QIEE63 pKa = 4.13QEE65 pKa = 3.88LAKK68 pKa = 10.24RR69 pKa = 11.84IHH71 pKa = 6.2NGRR74 pKa = 11.84RR75 pKa = 11.84YY76 pKa = 9.27PP77 pKa = 3.53

Molecular weight: 9.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

23668

37

3760

260.1

29.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.981 ± 0.368

0.722 ± 0.149

6.659 ± 0.134

7.318 ± 0.251

3.76 ± 0.175

7.318 ± 0.274

1.927 ± 0.263

5.564 ± 0.179

5.581 ± 0.315

7.952 ± 0.232

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.291 ± 0.151

4.935 ± 0.204

4.365 ± 0.17

4.441 ± 0.19

4.91 ± 0.258

5.607 ± 0.154

6.371 ± 0.23

6.591 ± 0.178

1.407 ± 0.132

3.3 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski