Caulobacter phage CcrColossus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dolichocephalovirinae; Colossusvirus; Caulobacter virus CcrColossus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 448 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4JRI5|K4JRI5_9CAUD Uncharacterized protein OS=Caulobacter phage CcrColossus OX=1211640 GN=CcrColossus_gp044 PE=4 SV=1
MM1 pKa = 7.6TSQLCIADD9 pKa = 3.78EE10 pKa = 4.02NAALDD15 pKa = 3.87FLARR19 pKa = 11.84AVPALCDD26 pKa = 3.76AGIMPPTLDD35 pKa = 4.18ILDD38 pKa = 4.15SLAANPNAYY47 pKa = 9.84GLSRR51 pKa = 11.84VPTLAPLAAEE61 pKa = 4.01WAGPYY66 pKa = 7.66QTQANDD72 pKa = 3.89GEE74 pKa = 4.91GPTTVRR80 pKa = 11.84ALIVAALGADD90 pKa = 3.32EE91 pKa = 5.49SGNFDD96 pKa = 3.21IGFRR100 pKa = 11.84TYY102 pKa = 11.17AVNDD106 pKa = 3.81TEE108 pKa = 4.23HH109 pKa = 6.89WNLAGTPLEE118 pKa = 4.28GVPAYY123 pKa = 10.35SLYY126 pKa = 10.76LIRR129 pKa = 11.84EE130 pKa = 4.49GEE132 pKa = 4.03QTYY135 pKa = 8.51MCSLTPSSYY144 pKa = 11.24AYY146 pKa = 10.25GIQNVFLYY154 pKa = 9.73PDD156 pKa = 3.62QEE158 pKa = 4.23GLPAVNEE165 pKa = 4.13YY166 pKa = 10.83GQTAVEE172 pKa = 4.48AYY174 pKa = 9.22MDD176 pKa = 4.15AEE178 pKa = 4.15NPEE181 pKa = 4.19YY182 pKa = 10.56EE183 pKa = 4.42AVEE186 pKa = 4.08DD187 pKa = 4.37TYY189 pKa = 11.16FGYY192 pKa = 9.98MGDD195 pKa = 3.93RR196 pKa = 11.84EE197 pKa = 4.21ALDD200 pKa = 3.19RR201 pKa = 11.84RR202 pKa = 11.84EE203 pKa = 4.21AEE205 pKa = 4.06EE206 pKa = 5.01GDD208 pKa = 3.46VSEE211 pKa = 5.18RR212 pKa = 11.84LSFDD216 pKa = 2.83KK217 pKa = 11.15SVFRR221 pKa = 11.84VDD223 pKa = 5.33LSTLDD228 pKa = 3.73GFAASPANPRR238 pKa = 11.84EE239 pKa = 4.02TTAFADD245 pKa = 4.2FEE247 pKa = 4.46TLAAAFYY254 pKa = 10.55KK255 pKa = 10.44AACDD259 pKa = 4.6SAWEE263 pKa = 4.0DD264 pKa = 3.05AKK266 pKa = 11.28EE267 pKa = 3.98YY268 pKa = 10.95VSGNATEE275 pKa = 4.06PRR277 pKa = 11.84ILTGCC282 pKa = 3.89

Molecular weight: 30.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4JUG3|K4JUG3_9CAUD Uncharacterized protein OS=Caulobacter phage CcrColossus OX=1211640 GN=CcrColossus_gp105 PE=4 SV=1
MM1 pKa = 7.23PTRR4 pKa = 11.84ARR6 pKa = 11.84RR7 pKa = 11.84SRR9 pKa = 11.84AKK11 pKa = 10.19RR12 pKa = 11.84ALAPGQQEE20 pKa = 4.36KK21 pKa = 11.01ALGASPGIYY30 pKa = 9.13IVMEE34 pKa = 4.68LGMHH38 pKa = 7.2PYY40 pKa = 10.35SPPTDD45 pKa = 3.01IGRR48 pKa = 11.84TIAGHH53 pKa = 5.3EE54 pKa = 4.24VHH56 pKa = 6.93HH57 pKa = 6.25LTADD61 pKa = 3.65CPRR64 pKa = 11.84NHH66 pKa = 6.33SRR68 pKa = 11.84RR69 pKa = 11.84AAKK72 pKa = 10.2RR73 pKa = 11.84LRR75 pKa = 11.84HH76 pKa = 6.24AARR79 pKa = 11.84QYY81 pKa = 11.15DD82 pKa = 3.68RR83 pKa = 11.84QLICQEE89 pKa = 3.89VANFLDD95 pKa = 3.83ASLAAA100 pKa = 4.97

Molecular weight: 11.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

448

0

448

83195

22

2750

185.7

20.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.789 ± 0.206

0.95 ± 0.058

6.433 ± 0.152

6.294 ± 0.203

3.761 ± 0.079

8.176 ± 0.448

2.263 ± 0.1

4.956 ± 0.074

4.487 ± 0.156

7.998 ± 0.142

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.483 ± 0.077

3.231 ± 0.109

5.212 ± 0.103

3.434 ± 0.084

6.69 ± 0.235

5.369 ± 0.142

5.892 ± 0.194

6.69 ± 0.115

1.688 ± 0.064

3.203 ± 0.089
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski