Fluviicola taffensis (strain DSM 16823 / NCIMB 13979 / RW262)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Crocinitomicaceae; Fluviicola; Fluviicola taffensis

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4016 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F2IEW8|F2IEW8_FLUTR Uncharacterized protein OS=Fluviicola taffensis (strain DSM 16823 / NCIMB 13979 / RW262) OX=755732 GN=Fluta_0426 PE=4 SV=1
MM1 pKa = 7.75KK2 pKa = 10.59SPLQWLFVASITMVSGISFSQTRR25 pKa = 11.84YY26 pKa = 10.04VSTTGTDD33 pKa = 3.12VSNDD37 pKa = 3.27CQTPGNPCGTISHH50 pKa = 7.33AYY52 pKa = 9.73SEE54 pKa = 5.48AIADD58 pKa = 4.07DD59 pKa = 4.42TIKK62 pKa = 10.47IAPGNYY68 pKa = 9.62NLTSTLSLLQPVVIAALDD86 pKa = 3.9PMDD89 pKa = 5.0LPVITSSASDD99 pKa = 3.8VISVNSDD106 pKa = 2.96DD107 pKa = 3.71VTIMNLHH114 pKa = 6.62FEE116 pKa = 4.35MGLTASTGLRR126 pKa = 11.84GIVANTGDD134 pKa = 3.98FDD136 pKa = 3.99NLKK139 pKa = 10.07VSGNIFEE146 pKa = 4.59STNPISGTPNTNMVWTAFAISLVATSGDD174 pKa = 3.67VQNVTIEE181 pKa = 4.17DD182 pKa = 3.69NEE184 pKa = 4.42IIADD188 pKa = 3.53ATSNVFGRR196 pKa = 11.84GIFLGNGGTGLDD208 pKa = 3.85APGGTISGNTIKK220 pKa = 10.36AYY222 pKa = 10.51YY223 pKa = 9.57AIQSALIADD232 pKa = 4.68DD233 pKa = 5.87LSISDD238 pKa = 4.08NDD240 pKa = 3.18IDD242 pKa = 4.1GTVLIIAPKK251 pKa = 10.04AGVIVDD257 pKa = 4.12VEE259 pKa = 4.59DD260 pKa = 4.17NMLGNNTQMTPASYY274 pKa = 10.69YY275 pKa = 10.79AVLEE279 pKa = 4.06LRR281 pKa = 11.84SIEE284 pKa = 4.11VGDD287 pKa = 3.8VNVSGNQFSNYY298 pKa = 8.56VNIGLLSEE306 pKa = 4.08ASKK309 pKa = 11.02NVSVLGNIFTPDD321 pKa = 2.96AAASGFTSIMANTKK335 pKa = 10.6LMTNGAQGNTYY346 pKa = 10.26SDD348 pKa = 4.59EE349 pKa = 3.87ITIQGNTFNAGLTGQGTAILFADD372 pKa = 4.22HH373 pKa = 6.75YY374 pKa = 11.08GVNTPAFGNITIGGAGVTDD393 pKa = 3.59KK394 pKa = 10.89NTFNSNLGTYY404 pKa = 9.41IALDD408 pKa = 3.92EE409 pKa = 4.53LSGPSTAFPLWATYY423 pKa = 8.52PATTMAPFSQDD434 pKa = 2.69VEE436 pKa = 4.35AYY438 pKa = 10.17AIYY441 pKa = 10.94NNYY444 pKa = 9.74GLANFAAIEE453 pKa = 4.26AKK455 pKa = 10.93NNDD458 pKa = 3.41QSDD461 pKa = 3.64EE462 pKa = 4.21AGLGKK467 pKa = 9.98IYY469 pKa = 9.71LTAYY473 pKa = 10.0GEE475 pKa = 4.0NRR477 pKa = 11.84YY478 pKa = 10.03VSTTGTDD485 pKa = 3.48VLNTCTLPGSPCLTIAHH502 pKa = 7.59AISVAIDD509 pKa = 3.45EE510 pKa = 5.2DD511 pKa = 4.43SVIVYY516 pKa = 8.99PGSYY520 pKa = 10.94ALTSVLNIGQDD531 pKa = 3.79GLVLTASDD539 pKa = 4.24VNDD542 pKa = 3.75KK543 pKa = 10.87PVITTNQANIIDD555 pKa = 4.12VNAEE559 pKa = 3.9DD560 pKa = 3.65VTVNGFEE567 pKa = 4.32FRR569 pKa = 11.84MGLTNATGIKK579 pKa = 10.1GVVSTASNFNNLKK592 pKa = 9.8VSKK595 pKa = 10.75NDD597 pKa = 4.34FISTAALDD605 pKa = 3.92LEE607 pKa = 4.42GMVWSAFAVQLTSATGEE624 pKa = 3.85IDD626 pKa = 3.34TVEE629 pKa = 3.92ITDD632 pKa = 4.08NGITTAATNLNVFGRR647 pKa = 11.84GFYY650 pKa = 10.47LGSGSANGPGGLIANNQIQAYY671 pKa = 9.03YY672 pKa = 10.5AIQTANIATDD682 pKa = 3.64LTVEE686 pKa = 4.27SNDD689 pKa = 3.12INGISMFSAPLNNSLFDD706 pKa = 3.76VYY708 pKa = 11.72DD709 pKa = 3.58NTFSGANMNTNMTSDD724 pKa = 2.95SLYY727 pKa = 10.56AVVEE731 pKa = 3.85IRR733 pKa = 11.84GIASGSIEE741 pKa = 4.81FYY743 pKa = 11.16DD744 pKa = 3.55NTIEE748 pKa = 4.22GFSKK752 pKa = 10.36IGLLSSASKK761 pKa = 10.88DD762 pKa = 3.65VIVDD766 pKa = 3.62ANVFTPKK773 pKa = 9.52ATATAFTSLMANTKK787 pKa = 10.57LMTNGVQGNTYY798 pKa = 10.1SDD800 pKa = 4.48EE801 pKa = 3.78ITIIGNTFSAGATNGGTAIIFADD824 pKa = 3.72HH825 pKa = 6.55YY826 pKa = 11.31GVNTPAFADD835 pKa = 3.48ITVGGAATAEE845 pKa = 4.12KK846 pKa = 10.81NIFNASLGKK855 pKa = 10.81YY856 pKa = 9.04IVLDD860 pKa = 3.7NKK862 pKa = 10.14TGTSASEE869 pKa = 4.03PLWAVYY875 pKa = 10.22DD876 pKa = 3.86ATNMKK881 pKa = 9.98PVTQNVEE888 pKa = 3.57ALYY891 pKa = 11.33VNNTYY896 pKa = 11.2NFGSTGEE903 pKa = 4.21VEE905 pKa = 4.71LKK907 pKa = 11.02NIDD910 pKa = 3.64SVDD913 pKa = 3.42NVILGKK919 pKa = 10.85VILAYY924 pKa = 10.7SFVGLDD930 pKa = 3.52EE931 pKa = 4.52LTSADD936 pKa = 3.06ATLYY940 pKa = 9.53PNPAINAVTIEE951 pKa = 4.22LTDD954 pKa = 3.99LNAVVEE960 pKa = 4.38MTVVDD965 pKa = 3.79LVGNVVYY972 pKa = 10.92NSTINGSITVDD983 pKa = 3.34VSNLTSGVYY992 pKa = 8.58VVRR995 pKa = 11.84LNNKK999 pKa = 8.32GQVSSTRR1006 pKa = 11.84FVKK1009 pKa = 10.69NN1010 pKa = 3.09

Molecular weight: 105.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F2IG14|F2IG14_FLUTR Uncharacterized protein OS=Fluviicola taffensis (strain DSM 16823 / NCIMB 13979 / RW262) OX=755732 GN=Fluta_1643 PE=4 SV=1
MM1 pKa = 7.52IFKK4 pKa = 10.76LDD6 pKa = 3.56TTKK9 pKa = 10.83KK10 pKa = 9.77EE11 pKa = 4.13RR12 pKa = 11.84NTATNRR18 pKa = 11.84RR19 pKa = 11.84LVQWRR24 pKa = 11.84VMWLIEE30 pKa = 4.35HH31 pKa = 5.91STSHH35 pKa = 5.72QLLWCIDD42 pKa = 3.68SFVLRR47 pKa = 11.84NPPLRR52 pKa = 11.84QAPNRR57 pKa = 11.84YY58 pKa = 6.67TAALDD63 pKa = 3.93EE64 pKa = 4.21NHH66 pKa = 5.42QTTFRR71 pKa = 11.84QQ72 pKa = 3.44

Molecular weight: 8.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4016

0

4016

1385128

30

4221

344.9

38.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.14 ± 0.044

1.005 ± 0.017

5.159 ± 0.028

6.187 ± 0.046

5.302 ± 0.036

6.681 ± 0.04

1.779 ± 0.019

7.72 ± 0.031

6.99 ± 0.055

9.24 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.02

5.975 ± 0.041

3.56 ± 0.027

3.723 ± 0.022

3.481 ± 0.029

7.203 ± 0.039

6.289 ± 0.071

6.219 ± 0.033

1.153 ± 0.015

3.997 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski