Halomonas anticariensis (strain DSM 16096 / CECT 5854 / LMG 22089 / FP35)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas; Halomonas anticariensis

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4705 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S2KNV2|S2KNV2_HALAF Outer-membrane lipoprotein LolB OS=Halomonas anticariensis (strain DSM 16096 / CECT 5854 / LMG 22089 / FP35) OX=1121939 GN=lolB PE=3 SV=1
MM1 pKa = 7.0STPSASRR8 pKa = 11.84TLIIPLATLATGLAVGWFAADD29 pKa = 3.29QHH31 pKa = 6.79GDD33 pKa = 3.45SAATDD38 pKa = 3.43ALTNDD43 pKa = 4.11SEE45 pKa = 4.44PLTLEE50 pKa = 3.37EE51 pKa = 4.53TYY53 pKa = 10.61RR54 pKa = 11.84GEE56 pKa = 3.97ITSASALNANDD67 pKa = 3.41GSRR70 pKa = 11.84FEE72 pKa = 4.49RR73 pKa = 11.84LPLALEE79 pKa = 4.53ADD81 pKa = 3.76TLVQLEE87 pKa = 4.61LGGVLNGALALYY99 pKa = 9.98DD100 pKa = 3.63AEE102 pKa = 4.82GNFLAASANDD112 pKa = 5.15GIPVRR117 pKa = 11.84LRR119 pKa = 11.84QRR121 pKa = 11.84IAQDD125 pKa = 3.16GNYY128 pKa = 9.41VLAVSGRR135 pKa = 11.84DD136 pKa = 3.05RR137 pKa = 11.84HH138 pKa = 6.22SYY140 pKa = 10.32GPFRR144 pKa = 11.84ITGHH148 pKa = 5.06TLEE151 pKa = 4.56TQNSGLLTLDD161 pKa = 3.44TPVNGWLQDD170 pKa = 3.2ASNDD174 pKa = 3.72YY175 pKa = 10.61EE176 pKa = 4.6VEE178 pKa = 4.01IPQAGLYY185 pKa = 8.99TIDD188 pKa = 3.71MHH190 pKa = 9.15SDD192 pKa = 3.27DD193 pKa = 4.64LDD195 pKa = 4.99AYY197 pKa = 10.88LVLSGSNGVKK207 pKa = 10.69LEE209 pKa = 4.84DD210 pKa = 4.97DD211 pKa = 4.7DD212 pKa = 5.59SAGDD216 pKa = 3.59LNARR220 pKa = 11.84VSGFLDD226 pKa = 3.3AGRR229 pKa = 11.84YY230 pKa = 6.54QLEE233 pKa = 3.92ARR235 pKa = 11.84TAQGQEE241 pKa = 3.38QGYY244 pKa = 7.76YY245 pKa = 9.03TLEE248 pKa = 4.38LGTRR252 pKa = 11.84DD253 pKa = 3.87LPDD256 pKa = 5.77DD257 pKa = 4.45IEE259 pKa = 4.27LQNGGEE265 pKa = 4.11LALGQPVQGWYY276 pKa = 10.16SGEE279 pKa = 3.82GLEE282 pKa = 4.14YY283 pKa = 10.63RR284 pKa = 11.84LDD286 pKa = 3.46IAEE289 pKa = 4.17QALVTIDD296 pKa = 3.64MQSSEE301 pKa = 3.83FDD303 pKa = 3.22AYY305 pKa = 11.4LEE307 pKa = 4.07LSGNGVMIEE316 pKa = 3.95NDD318 pKa = 3.91DD319 pKa = 3.75GGGNYY324 pKa = 9.81DD325 pKa = 3.91SRR327 pKa = 11.84IQTVLSPGTYY337 pKa = 6.92TVRR340 pKa = 11.84AISYY344 pKa = 8.96DD345 pKa = 3.38SSGSGLFTLEE355 pKa = 4.64AGASDD360 pKa = 3.38IAAAEE365 pKa = 4.08NGVIEE370 pKa = 4.84IGTTVTGQLAGGSQDD385 pKa = 3.31YY386 pKa = 11.31YY387 pKa = 11.17SFHH390 pKa = 7.23VDD392 pKa = 2.45RR393 pKa = 11.84AGQYY397 pKa = 9.54RR398 pKa = 11.84IAMMSSDD405 pKa = 3.26VDD407 pKa = 3.7SYY409 pKa = 11.99LVLQGNGLYY418 pKa = 10.86LEE420 pKa = 5.64DD421 pKa = 5.82DD422 pKa = 4.63DD423 pKa = 6.92GGDD426 pKa = 3.69GYY428 pKa = 11.02NARR431 pKa = 11.84LQTHH435 pKa = 6.86LEE437 pKa = 4.15PGDD440 pKa = 3.69YY441 pKa = 10.85RR442 pKa = 11.84MTARR446 pKa = 11.84TYY448 pKa = 8.14DD449 pKa = 3.45TSGSGSYY456 pKa = 10.16SVSVNSADD464 pKa = 3.87PMLL467 pKa = 4.87

Molecular weight: 49.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S2KF15|S2KF15_HALAF Phosphate import ATP-binding protein PstB OS=Halomonas anticariensis (strain DSM 16096 / CECT 5854 / LMG 22089 / FP35) OX=1121939 GN=pstB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4705

0

4705

1492920

29

3848

317.3

34.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.519 ± 0.042

0.972 ± 0.013

5.739 ± 0.032

6.599 ± 0.035

3.578 ± 0.023

7.998 ± 0.033

2.553 ± 0.02

5.002 ± 0.034

2.795 ± 0.026

11.331 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.533 ± 0.017

2.682 ± 0.018

4.884 ± 0.027

3.826 ± 0.027

7.108 ± 0.039

5.615 ± 0.027

5.063 ± 0.024

7.188 ± 0.03

1.507 ± 0.016

2.507 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski