Enterococcus phage Ec-ZZ2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Efquatrovirus; Enterococcus virus EcZZ2

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A139ZVW6|A0A139ZVW6_9CAUD Putative prim-pol domain protein OS=Enterococcus phage Ec-ZZ2 OX=1647400 GN=ZZ2_004 PE=4 SV=1
MM1 pKa = 7.31YY2 pKa = 9.6IVNVIDD8 pKa = 5.5FEE10 pKa = 4.86TKK12 pKa = 8.33TLAYY16 pKa = 9.8QSEE19 pKa = 4.75SFTTKK24 pKa = 10.23EE25 pKa = 3.54LAEE28 pKa = 4.72DD29 pKa = 3.93YY30 pKa = 9.9MVEE33 pKa = 4.31VMEE36 pKa = 4.5NTHH39 pKa = 6.34GLDD42 pKa = 3.33VLIIEE47 pKa = 4.46VNSYY51 pKa = 10.57NLEE54 pKa = 3.87TAKK57 pKa = 10.33IVACSS62 pKa = 3.15

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A139ZVW8|A0A139ZVW8_9CAUD Uncharacterized protein OS=Enterococcus phage Ec-ZZ2 OX=1647400 GN=ZZ2_006 PE=4 SV=1
MM1 pKa = 7.7LSQRR5 pKa = 11.84IQRR8 pKa = 11.84AGYY11 pKa = 9.0YY12 pKa = 10.13VVDD15 pKa = 4.14LTLNKK20 pKa = 9.59QKK22 pKa = 9.35KK23 pKa = 7.0TKK25 pKa = 9.74LVHH28 pKa = 5.93RR29 pKa = 11.84LVVEE33 pKa = 3.96VFISDD38 pKa = 2.83IGTNVVNHH46 pKa = 6.54IDD48 pKa = 3.63EE49 pKa = 4.55NRR51 pKa = 11.84LNNHH55 pKa = 6.73LDD57 pKa = 3.2NLEE60 pKa = 3.89IVSQNKK66 pKa = 8.89NLHH69 pKa = 6.15KK70 pKa = 10.5SSKK73 pKa = 9.0FNKK76 pKa = 9.88YY77 pKa = 7.3MDD79 pKa = 4.38RR80 pKa = 11.84LRR82 pKa = 11.84QPITIYY88 pKa = 10.75DD89 pKa = 3.67KK90 pKa = 11.03QINSMMTFNSKK101 pKa = 8.76TEE103 pKa = 3.76ACDD106 pKa = 3.65YY107 pKa = 10.93YY108 pKa = 11.52NLSTSYY114 pKa = 11.08FSMLQSRR121 pKa = 11.84NKK123 pKa = 10.56GEE125 pKa = 3.9NNRR128 pKa = 11.84FKK130 pKa = 10.56IISEE134 pKa = 4.31EE135 pKa = 3.83NGKK138 pKa = 9.63RR139 pKa = 3.41

Molecular weight: 16.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

11861

33

1410

201.0

22.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.775 ± 0.371

0.582 ± 0.118

6.138 ± 0.207

8.102 ± 0.51

4.114 ± 0.236

6.399 ± 0.504

1.467 ± 0.172

6.88 ± 0.238

8.844 ± 0.379

8.17 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.942 ± 0.162

6.568 ± 0.305

2.917 ± 0.193

3.667 ± 0.149

3.482 ± 0.224

5.371 ± 0.261

6.416 ± 0.374

6.812 ± 0.241

1.214 ± 0.117

4.14 ± 0.338
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski